Heterologous proteins
Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae
... erologous proteins are fused to the C-terminus of Aga2p, Aga2p forms a complex with Aga1p via disulfide bonds, and Aga1p is anchored to the yeast surface through a GPI- ...the heterologous proteins ...
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Heat Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons
... deliver heterologous proteins into cells, virus-based and ...Current heterologous protein delivery platforms include protective antigen (PA) of anthrax toxin ...for heterologous protein ...
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Lactococcus lactis M4, a potential host for the expression of heterologous proteins
... Our results show that eight Lactococcus lactis milk strains were successfully isolated, characterised and were resistant to antibiotics from the groups of aminoglyco- sides, coumarin-glycosides and quinolones tested, in ...
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Surface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchor
... ing proteins by sequence homology ...ferent heterologous proteins (GFP or WlacD) onto the surface of the host cells, raising the possibility of cell sur- face display of other proteins, such ...
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BPMV based viral constructs useful for VIGS and expression of heterologous proteins in legumes
... The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The ...
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A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
... of heterologous proteins, especially those containing disulfide bonds for diagnostic, therapeutic and other ...target proteins, and offer several advantages in improving the secretory and extracellu- ...
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Developing Gram-negative bacteria for the secretion of heterologous proteins
... of heterologous proteins at high titers and are robust in industrial-scale culture ...of proteins, particularly biomaterials, are toxic when expressed intracellularly in Gram-negative hosts, which ...
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Incorporation of homologous and heterologous proteins into the envelope of Moloney murine leukemia virus.
... M-MuLV spike proteins were efficiently assembled into extracellular M-MuLV particles when expressed at a relatively low density at the plasma membrane, whereas the efficient inclusion of[r] ...
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Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
... of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many transcription factors are utilized to address the burden caused by the high ...
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A structurally informed autotransporter platform for efficient heterologous protein secretion and display
... N-terminal fusion of ESAT6 to an Hbp passenger that was truncated down to residue 557 (HbpTr557), just downstream of side domain d2, resulted in a ~2-fold de- crease in the recovery of cleaved Hbp-ESAT6 passenger ...
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A review on Lactococcus lactis: from food to factory
... secrete heterologous proteins with efficien- cies comparable to Usp45 in ...of heterologous proteins, but on total pro- tein yield as ...
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Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production
... for heterologous protein delivery in foodstuff or in the digestive ...Many heterologous proteins have already been produced in ...several heterologous proteins produced in ...labile ...
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Heterologous Expression, Purification, and Characterization of the HspX, Ppe44, and EsxV Proteins of Mycobacterium tuberculosis
... The relatively high rate of protein expression in E. coli can result in misfolding, especially for heterologous proteins that require molecular chaperones or additional time to fold correctly (36). Cysteine ...
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The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli
... of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression ...
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Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains
... of heterologous proteins due to several ...secrete proteins directly into the extracellular medium, resulting in cost-effective downstream purification pro- ...accumulate proteins ...
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Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins
... of heterologous DNA, the presence of a cytoplasmic site of transcription, and high expression ...late proteins was ...of heterologous genes regulated by a T7 ...the heterologous gene ...
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Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain
... recombinant proteins for downstream utilization is a major ...overexpressing heterologous proteins ...some proteins and the formation of inac- tive insoluble ...
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Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
... biopharmaceutical proteins as well as for bulk ...of heterologous proteins [1], and has come into focus for metabolic engineering applica- tions ...of heterologous proteins with ...
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Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels
... produce heterologous proteins in the periplasm rather than in the cytoplasm of ...of proteins from the peri- plasm is usually easier than the isolation of proteins from total cell lysates, ...
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Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica
... of proteins (Beckerich et ...of heterologous proteins on ...of heterologous proteins on ...for heterologous pro- tein expression as it operates almost unaffected by envir- ...
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