high cell density cultivation
High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production
... the cell wall, the capsular polysaccharide could be considered a biomass related product, but its biosynth- esis is strictly regulated by environmental and growth con- ditions ...of high biomass ...
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High cell density cultivation on Hendersonia sp. for the application of biological control
... Hendersonia has been researched for its biological control properties, especially in controlling fungal diseases. Resulting from this, there is a growing need of these fungi in oil palm cultivations across Malaysia and ...
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High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor
... Background: Single-use rocking-motion-type bag bioreactors provide advantages compared to standard stirred tank bioreactors by decreased contamination risks, reduction of cleaning and sterilization time, lower investment ...
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Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations
... the cultivation. Unlike our previous results that high cell densities generated high valinomycin concen- tration in the shake flask scale [18], a high cell density ...
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Improved productivity of poly (4-hydroxybutyrate) (P4HB) in recombinant Escherichia coli using glycerol as the growth substrate with fed-batch culture
... the high cost of P4HB hinders its wider applications ...of high cell density (higher than 20 g L −1 ) processes to reach high P4HB ...productivities. High prod- uctivity can be ...
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A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures
... ent cultivation formats (shake flasks and 24-deep well plates), that the novel EnBase Flo system provides high cell densities without a decrease in recombinant protein productivity per ...of ...
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Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
... Our results confirm the importance of applying the fed- batch mode from the beginning of process development. Using different conditons during initial screening and later in the bioreactor scale leads to selection of ...
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Autotrophic Growth of Paracoccus denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO2
... rpm. High cell density culture experiment was carried out using a glass jar fermenter (total volume 1000 mL; working volume 600 mL) equipped with a pH controller, PHC-2201 and a dissolved oxygen (DO) ...
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Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode
... (40 bp) were electroporated into E. coli W3110 carrying pKD46 where lambda recombinase was fully induced by growth on L-arabinose during cultivation at 30°C. 2 h after electroporation and incubation at 37°C, cells ...
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Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density
... and maximized magnetosome formation by Magnetospiril- lum gryphiswaldense strain MSR-1 at low dissolved oxygen concentration and carbon source limitation. pH-stat feed- ing is a feeding strategy based on a pH feedback ...
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Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli
... their high content of lactose and salts whey has been proposed as cultivation additive for microbial cultivations, but so far it is mainly used for the production of lactic acid or used as animal ...
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Study of different factors affecting Agrobacterium- mediated genetic transformation in lentil (Lens culinaris Medik.)
... It is clear that there is a need to increase productivity, enhance the nutritional value and other desired agronomic quality of this crop. Cultivars resistant to biotic and abiotic stresses and better protein quality and ...
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Monitoring of fermentations with online capacitance sensor
... viable cell count determinations play key- roles both in fermentation industry and ...living cell numbers), furthermore they are less sensitive sensor against external influences with robust ...in ...
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Cost of Cultivation and Profitability of Agriculture in West Bengal: A Study with Special Reference to Backward Region of West Bengal
... (` 422.79) and cauliflower (` 408.37) cultivation while paddy (` 19.37) and pulses (` 89.63) is lowest. In the winter season vegetables like cauliflower, potato and brinjal were more profitable than paddy and ...
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High yield expression of proteins in E coli for NMR studies
... report high yield target protein expre- ssion with Maltose binding protein (MBP) [19], Gluta- thione-S transferase (GST) [20], Thioredoxin fusion (TRX) [21], Hexahistidine His6-Tag [19,22], Small ubiquitin- ...
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Variability in Wood Density and Wood Fibre Characterization of Woody Species and Their Possible Utility in Northeastern Mexico
... wood density and its utilization northeastern Mexico for different purposes and to find possi- ble relationship of density with wood fibre ...with high wood density for possible ...wood ...
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Expression of micro-RNAs and genes related to angiogenesis in ccRCC and associations with tumor characteristics
... Some limitations of our study should be mentioned. This is a retrospective study and the number of patients is low in the control group and would be interesting to validate the results with a larger number of controls ...
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Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells
... specialized cell types and their extensive capacity for self- ...stem cell populations, human pluripotent stem cells (hPSCs) can in principle be differentiated into any somatic cell type and exhibit ...
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Effect of Diffuser Aerator to Increase Growth of Nilem (Osteochilus vittatus) Cultivation With Different Density
... nilem cultivation can be done by high dense dispersion with the addition of input diffuser aerator ...stocking density on the cultivation of nilem (Osteochilus vittatus) given diffuser ...
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Organic turmeric cultivation in the eastern Ghat high land zone of Odisha
... first in turmeric area as well as production (28,828 t from 11,088 ha) while Koraput is the second largest producing district with 7,761 t from 3,168 ha (Babu et al., 2015). The chemical method of farming is becoming ...
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