High mannose N-glycans
Alteration of liver N glycome in patients with hepatocellular carcinoma
... desialylated N-glycans in liver N-glycoproteins was different from that of the serum N-glycan finger print (Figure ...abundant N-glycans, represented as L1, L2 and L3 (Fig- ure ...
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Resistance of Human Immunodeficiency Virus Type 1 to the High-Mannose Binding Agents Cyanovirin N and Concanavalin A
... cyanovirin N (CV-N), an 11-kDa protein originally isolated from the cyanobacterium Nostoc ellipsospo- rum, was found to target high-mannose glycans in an oligosac- charide-dependent ...
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Travelling-wave ion mobility mass spectrometry and negative ion fragmentation of hybrid and complex N-glycans.
... larger glycans, however, although existing as isomers, produced only asymmetric rather than separated arrival time distributions ...on high-mannose glycans, usually suggested that separations ...
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Variable Surface Epitopes in the Crystal Structure of Dengue Virus Type 3 Envelope Glycoprotein
... first N-acetylglucosamine of the Asn-67 glycan, but up to six sugar rings are visible in the electron density for the Asn- 153 glycan of the A ...two N-acetylglucosamines with a fu- cose on the first ...
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Location-Specific, Unequal Contribution of the N Glycans in Simian Immunodeficiency Virus gp120 to Viral Infectivity and Removal of Multiple Glycans without Disturbing Infectivity
... Asn-Xaa-Thr/Ser N- linked glycosylation sequences to a noncanonical residue, dem- onstrated that the 23 N-linked glycosylation sites of SIVmac239 gp120 were not equally important for viral ...the N ...
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Differential Inhibitory Effects of Cyanovirin-N, Griffithsin, and Scytovirin on Entry Mediated by Envelopes of Gammaretroviruses and Deltaretroviruses
... retroviruses, N-linked gly- cans are responsible for a substantial portion of the molecular mass of ...SU. High-mannose glycans are attached to an asparagine residue within a conserved motif ...
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Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
... FIG 3 GnTI-SIV is more sensitive to neutralization by soluble, mannose-specific lectins and is better transmitted by mucosal lectins than wt SIV. (A) Infectivity-normalized stocks of wt SIV, GnTI-SIV, HIV-1 NL4-3 ...
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Yeast-Elicited Cross-Reactive Antibodies to HIV Env Glycans Efficiently Neutralize Virions Expressing Exclusively High-Mannose N-Linked Glycans
... recognizing high-mannose glycans on HIV-1 ...15 N-linked consensus ...eliminating glycans that are not in- volved in 2G12 binding on each protein, it may be possible to focus the immune ...
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Maturation of Human Herpesvirus 6A Glycoprotein O Requires Coexpression of Glycoprotein H and Glycoprotein L
... of glycans and/or other moieties and, in some cases, by cleavage of the glycoproteins ...the N-linked oligosaccharides on the glycoproteins are modified from high-mannose oligosaccharides into ...
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High-Mannose Glycan-Dependent Epitopes Are Frequently Targeted in Broad Neutralizing Antibody Responses during Human Immunodeficiency Virus Type 1 Infection
... potential N-linked glycosyla- tion ...exclusively N-linked glycans (N-glycans), with a minimal level of O-linked glycans (4, 15, 21, 36, 37, ...47). N-glycans have ...
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Development of novel galactosylation method for the expression of recombinant human transferrin in insect cell culture
... complex-type N- ...complex-type N-glycans in insect cells may increase the value of insect cell derived products as vaccines, therapeutic and ...
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Novel Functions of Hendra Virus G N-Glycans and Comparisons to Nipah Virus
... predicted N-glycosylation sites in HeV ...(B) N-glycan structural positions in the head of HeV ...head N-glycosylation sites (G3 to G7 and position 275, highlighted in red and marked by red ...
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Mutational Analysis of the N-Linked Glycans onAutographa californica Nucleopolyhedrovirus gp64
... the N-linked glycans on AcMNPV gp64, characterize their structures, and evaluate their influence on gp64 ...consensus N-glycosylation sites in gp64 are used, and we mapped the positions of those ...
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Using the Man9(GlcNAc)2 – DC SIGN pairing to probe specificity in photochemical immobilization
... the capture of saccharides in order to probe their interactions with lectins. Molecular recognition of the immobilised sugars by DC-SIGN reproduced the known binding profile of this lectin, though mannose gave a ...
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The role of N-glycans in colorectal cancer progression: potential biomarkers and therapeutic applications
... that N-glycan biosynthesis is an appealing therapeutic target in cancer [94, ...95]. N-glycan biosynthesis can be blocked using compounds such as swainsonine or ...type N-glycans, and ...
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Human mannose binding protein activates the alternative complement pathway and enhances serum bactericidal activity on a mannose rich isolate of Salmonella
... human mannose-binding protein (MBP) is a multimeric serum protein that is divided into three domains, a cysteine-rich NH2-terminal domain that stabilizes the collagen alpha helix of the second domain and a third ...
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Relative Quantification of N-linked Glycans in Complex Mixtures via Stable Isotope Labeling and Enhanced Analysis by Liquid Chromatography Coupled Online to Mass Spectrometry.
... Peptide: N-Glycosidase F ...MI). High performance liquid chromatography grade acetonitrile (ACN), water, and methanol (MeOH) were all purchased from Burdick & Jackson (Muskegon, ...
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Hepatitis B Virus Large Envelope Protein Interacts with γ2-Adaptin, a Clathrin Adaptor-Related Protein
... Yeast two-hybrid screening. A MATCHMAKER human liver cDNA library fused with the yeast GAL4 activation domain (AD) in plasmid pACT2 was purchased from Clontech Laboratories and screened according to the manufac- turer’s ...
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Mannose corrects altered N glycosylation in carbohydrate deficient glycoprotein syndrome fibroblasts
... tained from American Radiolabeled Chemicals, Inc. (St. Louis, MO). Cell lines. The CDGS and control cell lines were all obtained from Dr. Neil Buist (Department of Pediatrics, Oregon Health Sci- ences University, ...
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Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins
... 5 N-linked glycosylation sites and E2 contains up to 11, with most of the sites being well conserved, suggesting that they play an essential role in some functions of these ...these glycans. The mutants ...
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