High quality RNA
Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis
... Conventional RNA isolation techniques are based on a ...isolating RNA from thousands of samples in micro-cen- trifuge tube format is incompatible with modern demands for high-throughput ...isolating ...
10
Isolation of high-quality RNA from a wide range of woody plants
... of high-quality RNA is one of the most crucial methods in molecular ...biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large ...
10
Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue.
... of high quality RNA suitable for whole genome expression profiling via microarray [1] or RNA sequencing [2] technologies presents a particular challenge due to the ubiquity of ...degrade ...
18
Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis
... of RNA for downstream micro- array analysis (Table 1). The highest quality RNA was obtained when the entire LCM process was ≤30 min, ...of quality control for all of the 42 gene chips ...
13
Efficient and High-Quality RNA Isolation from Metabolite-Rich Tissues of Stevia rebaudiana, an Important Commercial Crop
... extracting high quality RNA from plant is strongly dependent on nature of the tissue, condition of the sample and ...for RNA extraction, it is very unlikely to have a single standard protocol ...
11
High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy
... detecting cellular changes leading to mRNA or protein modulations. For example, viral infections in the mos- quito midgut often are not systemic but form isolated infection foci [20]. If entire mosquitoes or abdomens are ...
9
Rapid and Reliable Method of High Quality RNA Extraction from Diverse Plants
... This high throughput plant RNA extraction protocol can be used to isolate high quality RNA from diverse plants for real time PCR and other downstream applications... Keywords RNA Extract[r] ...
12
An Efficient Method for High Quality RNA Extraction from Moringa oleifera
... One gram of plant leaf tissue is homogenized in a guanidine hydrochloride - buffer containing 6.5 M guanidine hydrochloride, 100 mM Tris-Cl (pH 8.0), 100 mM sodium acetate (pH 5.5), and 0.1 M β-mercaptoethanol (added ...
7
A fast and efficient method for preparation of high quality RNA from fungal mycelia
... Mulgrave, VIC, Australia) was added and the sample ground further until the slurry had thawed. The sample was split into two equal volumes and transferred to 2 ml Eppendorf tubes. After 5 min incubation at room ...
5
Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
... obtain high quality RNA from any type of tissue, optimization of steps that precede the LCM process are ...stains RNA, and thus the endoplasmic reticulum and ribosomes blue ...the RNA ...
6
Q&A: using Patch-seq to profile single cells
... the RNA con- tained in a single cell (estimated to be ~10 ...the quality of the tissue slice, cell, and the patching steps themselves are all important to be able to isolate high-quality ...
7
Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples
... The primary aim of this study was to develop a technique to isolate IECs from mucosal biopsies obtained from patients with ulcerative colitis (UC) and healthy volunteers in order to successfully study gene expression in ...
9
A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
... the RNA environment within the cells and ...produces high- quality RNA from laser microdissected floral tissue from the phylogenetically distant species ...
11
83. Comparative profiling of gossypol enantiomers and seed traits’ related transcripts in cotton lines
... contain high O- methylated gossypol derivatives favoring the existence of (-)- gossypol ...22]. RNA quality and integrity is important in molecular analyses, such as RT-PCR, cDNA library ...
11
An improved method for RNA extraction from woody legume species Acacia koa A. Gray and Leucaena leucocephala (Lam.) de Wit
... extract high-quality RNA of sufficient quantity from the stem tissues of tree legumes, such as Acacia koa and Leucaena leucocephala, because they contain high amounts of phenolic compounds and ...
6
Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
... total RNA with good yield, high integrity and minimal genomic DNA ...extract high quality RNA with minimal deg- radation that are suitable for sequencing ...total RNA extraction ...
11
A Simple and efficient protocol for isolation of RNA from different tissues of chickpea and pea
... of high quality RNA from roots and leaves of chickpea and ...for RNA isolation from pea leaves only, and yields excess amount of high-quality RNA suitable for cDNA based ...
6
Piskol, Robert (2011): Structural and population genetic determinants of RNA secondary structure evolution. Dissertation, LMU München: Fakultät für Biologie
... of RNA secondary structure and the applicability of Kimura’s model were demonstrated in many ...sufficiently high chance to act such that a distance effect would be ...alignment quality due to the ...
130
journal.ppat.1001163.PDF
... of RNA synthesis by amiloride is dose ...of RNA synthesis of wild type virus treated with different concentrations of ...and RNA was extracted 48 hours after ...in high concentrations of Mg 2+ ...
14
RNA-RNA interaction prediction using genetic algorithm
... find RNA-RNA ...two RNA molecules under a number of energy models with growing com- plexity was introduced in ...each RNA and between the two ...
7