High resolution melt (HRM)-PCR
Differentiation of Solenopsis invicta social forms using high resolution melt PCR.
... PCR-RFLP The restriction endonuclease digestion of the purified 390 bp amplicons yielded a visible restriction pattern from the PCRs. All patterns correlated with the HRM results, where the BB homozygotes were ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... a high-resolution melt (HRM) PCR assay for the differ- entiation of the four serovars of ...for PCR amplification and HRM analysis to generate reproducible and distinct melt ...
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A PCR–High Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
... a PCR– high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus ...assay. PCR-HRM is ideal for ...
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Real Time PCR and High Resolution Melt Analysis for Rapid Detection of Mycobacterium leprae Drug Resistance Mutations and Strain Types
... real-time PCR tech- nologies that can eliminate post-PCR procedures for genotyping any ...Real-time PCR– high- resolution melt (PCR-HRM) analysis is a novel simple ...
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Rapid identification of chloroquine and atovaquone drug resistance in Plasmodium falciparum using high resolution melt polymerase chain reaction
... and PCR, are now increasingly being ...5'nuclease PCR assay for detection and differentiation of drug resistance haplotypes for chloroquine and atovaquone using polymerase chain reaction-high ...
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... The current study reports a real-time PCR assay that is able to detect and identify six known C. psittaci genotypes, along with identifying C. caviae and C. abortus. This assay may aid in determining the existence ...
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Introduction to High Resolution Melt Analysis
... from PCR), and melt profiles are obtained directly after amplification, supplying information not obtained from low resolution melt ...
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... real-time PCR assays for the detection of different Brucella ...time PCR. All other existing real-time PCR-based assays iden- tify this species through indirect testing ...
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Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... real-time PCR and HRM analysis cannot detect drug resistance conferred by un- known mutations or by unknown mechanisms, and this limita- tion is common among all sequence-dependent detection ...
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High Resolution Melt analysis for mutation screening in PKD1 and PKD2
... range PCR assays were per- formed as described previously [18], LR1 amplified exon 1, LR2 amplified exon 2 to 12, LR3 amplified exon 13 to 15D, LR4 amplified exon 15E to 22, LR5 amplified exon 22 to ...LR ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... the PCR master mix at a final concentration of 0.6 M each. The PCR mixture was further com- posed of ...⫻ PCR buffer, ...and PCR-grade H 2 O to a final volume of 25 l with 20 ng template ...
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HRM. High Resolution Melt Assay Design and Analysis. CorProtocol July06
... to high copy ...the PCR in the presence of a dsDNA intercalating fluorescent ...is high in a melt analysis because the sample starts as dsDNA, but fluorescence diminishes as the temperature is ...
amplification tech A Practical Guide to High Resolution Melt Analysis Genotyping
... real-time PCR detection system with excellent thermal stability and sensitivity and HRM-dedicated ...DNA melt curve profiles that are both specific and sensitive enough to distinguish nucleic acid species ...
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... real-time PCR HRM assay developed in this study is less complicated than the assay developed by Stermann and colleagues (13) as it does not require flourescence-labeled probes or combined prim- ers and ...
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Simultaneous Detection of Colletotrichum acutatum and C gloeosporioides from Quiescently Infected Strawberry Foliage by Real Time PCR Based on High Resolution Melt Curve Analysis
... Real-time PCR primer/probe specificity testing resulted in the amplification of the target Colletotrichum species but none of the non- Colletotrichum ...time PCR with those tissues when used as negative ...
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Rapid High Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis
... nucleotides. High-resolution melt curves and differential fluorescence curves of 69 samples are shown for one of three replicate PCRs for each of the five nucleotide posi- tions ...on melt ...
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High Resolution Melt Analysis of Samples with Differential DNA Methylation to Identify Tissue Source of Origin
... high-priced due to materials, equipment, and other ...the PCR and HRM Analysis in one ...the PCR reaction volume for each sample to be analyzed and eliminates the need for solvents and ...
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Speciation of common Gram negative pathogens using a highly multiplexed high resolution melt curve assay
... as PCR have been used, they often struggle with the challenge of detecting and discriminating a wide range of ...targets. High resolution melt analysis is an end-point qPCR detection method ...
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Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay
... as PCR have been used, they often struggle with the challenge of detecting and discriminating a wide range of ...targets. High resolution melt analysis is an end-point qPCR detection method ...
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High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
... High resolution melt (HRM) analysis is a technique that measures the disassociation of double-stranded DNA at high temperature resolution, and permits the analysis of genetic variations ...
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