High-throughput molecular markers
Allelic effects and variations for key bread-making quality genes in bread wheat using high-throughput molecular markers
... Received Date: 24 June 2018 Revised Date: 4 December 2018 Accepted Date: 7 December 2018 Please cite this article as: Rasheed, A., Jin, H., Xiao, Y., Zhang, Y., Hao, Y., Zhang, Y., Hickey, L., Morgounov, A.I., Xia, X., ...
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Development of High throughput Molecular Markers for Blackleg (Leptosphaeria maculans) Resistance Genes in Brassica napus for Gene Stacking
... For the SNP development, ten genes (Table 1) from a N7-orthologous-region on Arabidopsis chromosome 1 were selected for primer design [47]. The specific PCR was performed at 94°C 50 s, 50-60°C 50 s and 72°C 50 s for 35 ...
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Towards high throughput molecular detection of Plasmodium: new approaches and molecular markers
... standard molecular diagnosis methods, the ultimate goal of this work is to develop new approaches allowing analysis of thousands of field ...higher throughput detection of the cyto- chrome b PCR products by ...
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High Throughput Assays for Mouse Metabolic Markers: Insulin, Leptin and Adiponectin
... LEPTIN • 16 kD molecular weight. Mice that lack functional leptin are obese, diabetic, infertile, and have reduced activity, metabolism, and body temperature. Circulating levels of leptin are regulated in response ...
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Clinical application of high throughput molecular screening techniques for pharmacogenomics
... CYP2D6 genotyping for multiple drug metabolism The CYP450 enzyme system is one of the most important determinants of drug metabolism in humans. One of the central components of this pathway is the enzyme encoded by the ...
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Fungal community analysis by high-throughput sequencing of amplified markers - a user's guide
... of high-throughput methods rather lies in the capacity to provide information about the main fungal colonizers in large numbers of samples, to a progressively decreasing cost in terms of money and ...
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Identification of Microsatellite Markers for Plasmopara viticola and Establishment of High throughput Method for SSR Analysis
... grapevine, molecular marker Abstract The Oomycete Plasmopara viticola is the causal organism of downy mildew on grapevine (Vitis ...microsatellite markers for P. viticola were developed. Five ...
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MOLECULAR MARKERS IN WHEAT
... These markers (RFLP) are specific to single clone/ restriction enzyme combinations and most of them are co-dominant in nature, highly locus specific and often ...These markers can be used across a wide ...
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Miniaturizing High Throughput Droplet Assays For Ultrasensitive Molecular Detection On A Portable Platform
... Miniaturizing High Throughput Droplet Assays For Ultrasensitive Molecular Detection On A Portable Platform Abstract Digital droplet assays – in which biological samples are compartmentalized into ...
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Original Article Six miRNAs identified serving as prognostic and predictive markers for osteoporosis by miRNA high-throughput method
... Keywords: Differentially expressed miRNA, osteoporosis, miRNAs-seq, functional analysis Introduction Primary osteoporosis is the most common and aggressive adult bone tumor over aged 65. It’s estimated that half of all ...
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Molecular markers in the improvement of Allium crops
... exploit high throughput SNP genotyping, functional genomics using RNAi or other mutagenic methods and tran- scriptome mapping to know the function of each gene in Allium ...
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Optimization of molecular tools for high-throughput genetic screening
... strategies could be implemented to further increase the efficacy of these tools. For both shRNAs and sgRNAs, design algorithms rely on large efficacy datasets and their accuracy depends on the quality and size of that ...
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Efficient high throughput molecular method to detect Ehrlichia ruminantium in ticks
... The performance of pCS20 Sol1 TqM qPCR was tested by comparing the results of detection using African ticks collected in the field and infected tick lysates with those obtained with pCS20 nested PCR and MLST. Among the ...
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Chronic lymphocytic leukemia: molecular heterogeneity revealed by high-throughput genomics
... Figure 1. In recent years, CLL has been investigated through the use of several novel genomic technologies. CLL is a disease of mature B cells that is typically present in high abundance in blood; a typical ...
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Chronic lymphocytic leukemia: molecular heterogeneity revealed by high-throughput genomics
... Figure 1. In recent years, CLL has been investigated through the use of several novel genomic technologies. CLL is a disease of mature B cells that is typically present in high abundance in blood; a typical ...
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Original Article Application of high-throughput protein array in clinical screening for tumor markers
... of high-throughput protein array in clinical screening for tumor ...tumor markers was ...had high sensitivity and specific- ity for interpreting protein array ...tumor markers were ...
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Ultra-high-throughput DArTseq-based silicoDArT and SNP markers for genomic studies in macadamia
... SNP markers were reported, of which 11,526 silicoDArT and 3,956 SNP markers were used for analyses after screening with quality control parameters including >95% call rate, >95% reproducibility, and ...SNP ...
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High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination
... SSAP markers have allowed the integration of Pisum genetic maps from different populations espe- cially where there are common parents ...as markers for mapping and diversity analysis in a wide range of ...
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Molecular Markers in Neuroblastoma
... the molecular characteristics of tumor ...prognostic molecular makers for extensive heterogeneity of ...genetic markers by which individual neuroblastomas are biologically charac- ...
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High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers.
... of high-content imaging in primary screening is becoming more widespread, and by utilising phenotypic approaches it should be possible to incorporate cytotoxicity counter-screens into primary ...Operetta ...
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