Hiv-1 Protease
Construction of a Human Immunodeficiency Virus Type 1 (HIV-1) Library Containing Random Combinations of Amino Acid Substitutions in the HIV-1 Protease due to Resistance by Protease Inhibitors
... type 1 (HIV-1) protease. The HIV-1 protease contains 99 amino acids and functions in a dimeric form for processing of the Gag and Gag-Pol polyproteins during viral ...
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Design and synthesis of inhibitors for the HIV 1 protease
... Although these cyclic alcohols did not possess the desired sp^ geometry at Pi and P^. (see Figure 2.15) they provided useful leads. Reduction of the double bonds was attempted using hydrogen gas over a palladium/ carbon ...
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HIV-1 Protease-Substrate Coevolution in Nelfinavir Resistance
... type 1 (HIV-1) protease inhibitors (PIs) challenges the effectiveness of therapies in treating HIV-1-infected individuals and AIDS ...the protease (PR) that render the PIs ...
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Docking Studies of HIV 1 Protease with Phytochemicals from Mappia foetida
... When HIV enters the human body, its target is a subset of immune cells that contain CD4 ...effective HIV PR, HIV remains uninfectious because its work in HIV is to create mature protein ...
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HIV-1 Protease, Reverse Transcriptase, and Integrase Variation
... HIV-1 protease (PR), reverse transcriptase (RT), and integrase (IN) variability presents a challenge to laboratories performing genotypic resistance ...low-abundance HIV-1 ...
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Molecular Docking Studies; Dietary Compounds As HIV 1 Protease Inhibitors
... the protease and block its function, thereby suppressing the virus, which cannot transform to its mature, infectious ...form. HIV 1 Protease inhibitors are one class of drugs that target the ...
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Multi step inhibition explains HIV 1 protease inhibitor pharmacodynamics and resistance
... the protease gene achieve their overall resistance pheno- type by altering both the entry and postentry dose-response ...of HIV-1 Env. MLV-E requires cleavage of its short CT by HIV-1 ...
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Effects of HIV-1 protease on cellular functions and their potential applications in antiretroviral therapy
... Type 1 (HIV-1) protease inhibitors (PIs) are the most potent class of drugs in antiretroviral ...resistant HIV-1 strains that are resistant to many or all current ...study ...
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HIV-1 Protease Evolvability Is Affected by Synonymous Nucleotide Recoding
... the HIV-1 HXB2 strain ...MAX HIV-1 protease was generated by PCR amplification with a combination of three overlapping synthetic DNA oligonucleotides, as previously described ...MAX ...
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Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity.
... 99-amino-acid protease. No protease expression was ob- served when this gene was cloned into the pTacTac vector (data not ...the protease and a highly expressed ...the protease gene to that of ...
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HIV 1 protease induced apoptosis
... of HIV-1 PR on selected anti- and pro-apoptotic Bcl-2 family proteins involved in mito- chondrial outer membrane permeabilization (MOMP) using: (i) in vivo cleavage of cellular proteins in HeLa or HEK-293 ...
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Human immunodeficiency virus type 1 (HIV-1) protein Vif inhibits the activity of HIV-1 protease in bacteria and in vitro.
... for 1 h, and washed, and cell pellets were lysed directly in Laemmli sample buffer containing 4% sodium dodecyl sulfate ...visualize HIV-1-specific proteins; bound antibody was detected by ...
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HIV 1 protease inhibitor mutations affect the development of HIV 1 resistance to the maturation inhibitor bevirimat
... three protease backgrounds (HXB2, PR-1 and ...gag- protease recombinant HIV-1 samples to select for bevir- imat ...mutated protease, which is in line with our previous in vivo ...
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Generation and characterization of a human immunodeficiency virus type 1 (HIV-1) mutant resistant to an HIV-1 protease inhibitor.
... Viruses obtained from treated and untreated cultures were called the escape and passaged viruses, respectively, and were used to infect MOLT-4 cells to establish chronically infected pro[r] ...
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HIV 1 protease cleaves the serine threonine kinases RIPK1 and RIPK2
... fied HIV-1 PR can cleave RIPK1 and RIPK2 in various cell types, we next asked whether RIPK1 and RIPK2 are cleaved during HIV-1 ...dotyped HIV-1 ...
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The L76V mutation in HIV-1 protease is potentially associated with hypersusceptibility to protease inhibitors Atazanavir and Saquinavir: is there a clinical advantage?
... Figure 1 Resensitizing effects of the L76V mutation are visible in phenotype results: Phenotypic resistance analysis before and after manifestation of L76V in two representative patients ...
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Intracellular expression of human immunodeficiency virus type 1 (HIV-1) protease variants inhibits replication of wild-type and protease inhibitor-resistant HIV-1 strains in human T-cell lines.
... our HIV-1 challenge assays, these differences were not readily ...intermediate-dose HIV-1 HXB3 and NF6 infection experiments, both td PR variants D25N and KWW showed comparable anti-HIV ...
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Optimizing HIV-1 protease production in Escherichia coli as fusion protein
... mature HIV-1Pr was designed by in silico back translation of the amino acid sequence reported in the database (GenBank Accession ...file 1, Figure S1) ...
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Virological Response to Highly Active Antiretroviral Therapy in Patients Infected with Human Immunodeficiency Virus Type 2 (HIV 2) and in Patients Dually Infected with HIV 1 and HIV 2 in The Gambia and Emergence of Drug Resistant Variants
... analysis. HIV-2 RNA was extracted from 140 l of EDTA-plasma using the QIAamp viral RNA kit (Qiagen, Venlo, The Netherlands) and was eluted into 50 l nuclease-free ...entire HIV-2 protease and RT ...
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BioAfrica's HIV 1 Proteomics Resource: Combining protein data with bioinformatics tools
... [15], PROSITE [16] or ProDom [17]; perform similarity searches using the BLAST program available at Genbank [18], conduct structural comparisons using the BioAfrica BLAST Structure program; determine amino acid compo- ...
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