HIV-1 RNA
Identification of Acute HIV 1 Infection by Hologic Aptima HIV 1 RNA Qualitative Assay
... low HIV-1 RNA-concentration speci- mens ...30 HIV-1 RNA copies/ml (32); thus, dilution of low concentration specimens would not be expected to compromise ...Quantitative ...
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The virion associated incoming HIV 1 RNA genome is not targeted by RNA interference
... the HIV-1 RNA genome of incoming particles using our lenti- viral vector transduction ...of HIV-1 replication ...wildtype HIV-1 ...incoming RNA genome with other ...
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Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... LCx HIV RNA Quantitative ...the HIV-1 pol region, which enabled this assay to detect HIV-1 of the M group (A to G subtypes) and the O ...and HIV-1 primers were ...
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Distribution of Chemokine Receptor CCR2 and CCR5 Genotypes and Their Relative Contribution to Human Immunodeficiency Virus Type 1 (HIV-1) Seroconversion, Early HIV-1 RNA Concentration in Plasma, and Later Disease Progression
... type 1 (HIV-1) seroconversion intervals and no potent antiretroviral therapy, mean plasma HIV-1 RNA level during the initial 42 months after seroconversion was higher in carriers ...
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Impact of Human Immunodeficiency Virus Type 1 (HIV 1) Genetic Diversity on Performance of Four Commercial Viral Load Assays: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR v1 5, VERSANT HIV 1 RNA 3 0, and NucliSens HIV 1 QT
... type 1 (HIV-1) evolution and changing strain distribution present a chal- lenge to nucleic acid-based ...diverse HIV-1. A panel of 97 HIV-1-seropositive plasma samples ...
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Performance comparison of new Veris and Xpert random access HIV 1 RNA quantification assays
... statistical analysis. The number of samples with inter-assay differences exceeding the clinical cutoff value of 0.5 log cp/mL was also recorded. Indeed, fluctuation of HIV-1 RNA VL over 0.5 log cp/mL ...
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Comparative evaluation of NASBA HIV 1 RNA QT, AMPLICOR HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... specific HIV-1 RNA assay can be ...viral RNA isolation procedure is less time-consuming than the gua- nidinium extraction method used by NASBA and AMPLICOR but uses a large amount of plasma, ...
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In Vitro and In Vivo Analysis of the Interaction between RNA Helicase A and HIV-1 RNA
... to HIV-1 RNA during viral ...the HIV-1 RNA bound by RHA during viral replication, an RNA-protein coprecipitation assay was used ...BH10 HIV-1 proviral DNA ...
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A Novel PCR Assay for Quantification of HIV-1 RNA
... genomic HIV-1 RNA and all spliced HIV-1 RNAs but not aberrant RNAs, integrated or unintegrated proviral DNA, or recombi- nant plasmids containing HIV-1 ...all ...
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An RNA binding compound that stabilizes the HIV 1 gRNA packaging signal structure and specifically blocks HIV 1 RNA encapsidation
... HIV-1 RNA has been suggested to adopt a range of different conformers, with each one playing a different role in the viral lifecycle [10, 42, ...the RNA is ...the HIV-1 leader ...
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Coamplification of HIV 1 Proviral DNA and Viral RNA in Assays Used for Quantification of HIV 1 RNA
... Amplicor HIV-1 Monitor version ...of HIV-1 amplifies a 143-bp segment in the conserved gag (group antigen) region of the HIV-1 ...the HIV-1 viral genome contains ...
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Binding of human immunodeficiency virus type 1 (HIV-1) RNA to recombinant HIV-1 gag polyprotein.
... The gag polyprotein is cleaved into the mature viral proteins by the viral protease at the time of particle budding 71, but it is probably the gag precursor polyprotein and not any of it[r] ...
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Pediatric Viral Human Immunodeficiency Virus Type 1 RNA Levels, Timing of Infection, and Disease Progression in African HIV-1-Infected Children
... having HIV-1 infection (53 from the 049a trial, 5 from the 049b trial, and 27 from the ZDV ...⫽ 1), and negative bDNA/positive DNA PCR pattern at month 9 and positive HIV serology at month 18 ...
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Comparison of LCx with Other Current Viral Load Assays for Detecting and Quantifying Human Immunodeficiency Virus Type 1 RNA in Patients Infected with the Circulating Recombinant Form A/G (CRF02)
... detect HIV RNA subtypes A, E, and G and other minor strains (7, 8, 10, 11, 14, ...NucliSens HIV RNA QT assay in quantifying viremia for the circulating recombinant form of HIV-1 ...
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Design and Evaluation of a Human Immunodeficiency Virus Type 1 RNA Assay Using Nucleic Acid Sequence Based Amplification Technology Able To Quantify Both Group M and O Viruses by Using the Long Terminal Repeat as Target
... type 1 (HIV-1) RNA level in plasma or serum has become one of the most impor- tant markers for monitoring HIV-1-infected ...than HIV-1 DNA, it is the only evidence ...
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Impact of Early Antiretroviral Therapy on Detection of Cell Associated HIV 1 Nucleic Acid in Blood by the Roche Cobas TaqMan Test
... consenting HIV-1-infected individuals between 18 and 50 years of age who had been on ART therapy for at least 6 months (RV180 protocol; Miriam Hospital, Providence, RI) with undetectable HIV-1 ...
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An enhanced sensitivity branched DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma
... for 1 h in a refrigerated microcentrifuge (centrifuge ...colder. HIV-1 RNA was liberated from the virus pellets by addition of 220 m l of specimen diluent (100 mM HEPES [N-2-hydroxy- ...
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Multicenter Evaluation of the Performance Characteristics of the NucliSens HIV 1 QT Assay Used for Quantitation of Human Immunodeficiency Virus Type 1 RNA
... Currently, HIV-1 viral load assays are not FDA approved for the diagnosis of HIV ...acute HIV-1 disease prior to seroconversion or for the resolution of HIV-1 status in ...
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The mouse viral outgrowth assay: avatars for the detection of HIV 1 reservoirs
... cell-associated HIV-1 RNA ...no HIV-1 DNA, RNA or replication-competent virus was detected from PBMCs or cells isolated from the ileum, rectum, lymph nodes, bone marrow and ...
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Effects of specimen collection, processing, and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma
... of HIV-1 RNA in cell-free plasma collected in VACUTAINER ...if HIV-1 RNA levels in cell-free plasma collected and stored at 4°C in VACUTAINER EDTA-PPTs remained stable after one ...
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