HIV-1 vif
The Structural Interface between HIV-1 Vif and Human APOBEC3H
... the Vif binding site of ...to HIV-1 Vif-mediated degradation (14, ...the HIV-1 Vif- binding site, we introduced positively charged arginine residues and negatively charged ...
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APOBEC3F Determinants of HIV-1 Vif Sensitivity
... FIG 1 Vif-A3F interaction model and A3F experimental ...from HIV-1 Vif sensitive to Vif resistant (11), multiple mutations may be required to sensitize rhA3F to ...
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MDM2 is a novel E3 ligase for HIV 1 Vif
... into HIV-1 virions and inhibits HIV-1 replication by inducing G-to-A hypermutation in viral cDNA during reverse transcription ...[5-8]. HIV-1 Vif counteracts A3G by ...
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Structural Insights into HIV-1 Vif-APOBEC3F Interaction
... the HIV-1 Vif ...the Vif structure was modeled in silico onto the crystal structure with an extension of the pentapeptide RWNKP (residues 173 to 177) and used for ...the Vif structure, ...
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HIV 1 Vif and APOBEC3G: Multiple roads to one goal
... type HIV-1 vif+ virus are not known; but, current observations are that APOBEC3G confers a major deleterious effect to the HIV-1 genome when the Vif protein is ...Historically, ...
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Identification of a tripartite interaction between the N terminus of HIV 1 Vif and CBFβ that is critical for Vif function
... identify HIV-1 Vif determinants that are essential for interaction with CBFβ, we used a previously described panel of single or double-alanine substitution mutants of the first 60 amino acids of ...
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Interactions between HIV 1 Vif and human ElonginB ElonginC are important for CBF β binding to Vif
... EloB and EloC are two ubiquitous proteins that form a heterodimer complex. They were originally found in the Elongin (SIII) complex, which was proposed to contain three subunits: EloA, EloB and EloC [37,38]. EloA is ...
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HIV 1 Vif inhibits G to A hypermutations catalyzed by virus encapsidated APOBEC3G to maintain HIV 1 infectivity
... that Vif has the capability of directly inhibiting A3G cytidine deaminase activity in a degradation- independent ...of Vif, without the depletion of a deaminase domain, in an Escherichia coli ...to ...
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Determinants of Efficient Degradation of APOBEC3 Restriction Factors by HIV-1 Vif
... to 1:500; Thermo Scientific), or monoclonal mouse A3H (catalog no. 12155; 1:500 [obtained through the NIH AIDS Reagent Program]) and polyclonal rabbit ␣ ...to HIV-1 p24 (catalog no. 4250; ...
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Polyubiquitination of APOBEC3G Is Essential for Its Degradation by HIV-1 Vif
... translation. HIV-1 Vif was rapidly degraded by 80% in 120 min without A3G expression, while approximately 20 to 30% of Vif was degraded in 120 min when A3G or A3G20K/R was coexpressed ...that ...
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Identification of Specific Determinants of Human APOBEC3F, APOBEC3C, and APOBEC3DE and African Green Monkey APOBEC3F That Interact with HIV-1 Vif
... on HIV-1 ...no Vif, WT Vif, or the F ...WT HIV-1 Vif on the degradation of WT hA3F or its ...no Vif or WT Vif expression ...of Vif to hA3F ...WT ...
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Simian Immunodeficiency Virus Vif and Human APOBEC3B Interactions Resemble Those between HIV-1 Vif and Human APOBEC3G
... protein Vif to counteract A3 enzymes and prevent restriction. Vif hijacks cellular core-binding factor beta (CBF-  ) to form an E3 ubiquitin ligase complex with Cullin-5 (CUL5), Elongin B (ELOB), ELOC, and ...
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APOBEC3G Impairs the Multimerization of the HIV-1 Vif Protein in Living Cells
... to Vif, we also checked whether they affect the intracellular localization of ...two Vif fusion proteins in the same cellular compartments was further evidenced by the overlap of their emissions ...the ...
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HIV 1 Vif, APOBEC, and Intrinsic Immunity
... by Vif is mediated through a physical interaction with APOBEC3G that results in the exclusion of the deaminase from ...of Vif is generally accompanied by a reduction of the intracellular steady state ...of ...
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Productive Replication of vif-Chimeric HIV-1 in Feline Cells
... produce HIV-1 with low infectivity and that this is at least partially accounted for by minus-strand editing by two of the five fA3 proteins; the greatest activity was attributable to the two-domain fA3CH, ...
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HIV 1 Escape from Small Molecule Antagonism of Vif
... of HIV-1 Vif function and restoration of A3G levels in nonpermissive H9 cells ...Fig. 1. HIV-1 replication was potently inhibited in a dose-dependent manner in nonpermissive ...
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Cellular and Viral Specificities of Human Immunodeficiency Virus Type 1 Vif Protein
... that HIV-1 Vif coun- teracts a cellular inhibitor that occurs specifically in nonper- missive human cells (22, 32), the current data strongly indicate that this increases the infectivity of ...
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The HDAC6/APOBEC3G complex regulates HIV 1 infectiveness by inducing Vif autophagic degradation
... degrade HIV-1 Vif and the restoration of A3G level in cells producing virions and HIV-1 infec- tiveness, regardless viral ...Indeed, HIV-1 infec- tion was impaired when ...
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Regulation of APOBEC3 Proteins by a Novel YXXL Motif in Human Immunodeficiency Virus Type 1 Vif and Simian Immunodeficiency Virus SIVagm Vif
... FIG. 1. A conserved YXXL motif in HIV-1 Vif is important for binding and neutralization of ...respectively. Vif residues examined for their potential role in A3G neutralization are ...
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The Cellular Antiviral Protein APOBEC3G Interacts with HIV-1 Reverse Transcriptase and Inhibits Its Function during Viral Replication
... cluding HIV-1 reverse transcriptase (RT) and Gag-p24, and encapsidated HA-A3G in each virus stock sample were detected by Western blotting (WB) analysis using corresponding antibodies ...of ...
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