HOECHST 33342
Use of Hoechst 33342 Staining To Detect Apoptotic Changes in Bovine Mononuclear Phagocytes Infected with Mycobacterium avium subsp. paratuberculosis
... Mycobacterium avium subsp. paratuberculosis is an intracellular pathogen of macrophages that causes a chron- ic enteritis (Johne’s disease) in ruminants. The purpose of this study was to determine whether M. avium subsp. ...
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Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
... Background: Simultaneous use of cell-permeant and impermeant fluorescent nuclear dyes is a common method to study cell viability and cell death progression. Although these assays are usually conducted as end-point ...
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The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil
... with Hoechst 33342 dye, which is sensitive to chromatin and is used to assess the changes in nuclear ...with Hoechst 33342 dye (5 μg/ml) for 20 min at room temperature in the ...the ...
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Disulfiram Reactivates Latent HIV-1 in a Bcl-2-Transduced Primary CD4+ T Cell Model without Inducing Global T Cell Activation
... Since agents that induce latent HIV-1 through global T cell activation are toxic, we confirmed that disulfiram does not cause global T cell activation. Increased cell size is a direct indicator of T cell activation. ...
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The Hippo component YAP localizes in the nucleus of human papilloma virus positive oropharyngeal squamous cell carcinoma
... Tissues were embedded in paraffin then cut in thin sec- tions and preserved for further staining. The sections were then deparaffinized in xylene followed by rehydra- tion through graded ethanol/water until rehydrated in ...
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<em>Hopea odorata </em>extract inhibits hepatocellular carcinoma via induction of caspase-dependent apoptosis
... with Hoechst 33342 to assess nuclei of all cells and with PI to assess nuclei of dead cells ...with Hoechst 33342 but not with PI; this means that there was no death cell in the control cells ...
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Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... around Hoechst 33342 is almost unchanged while sorption of this fluorescent dye on a sur- face of the hairpin oligonucleotide HP1, t-RNA and ...to Hoechst is absent due to their remoteness and also ...
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Effect of methyl-beta-cyclodextrin on the viability and acrosome damage of sex-sorted sperm in frozen-thawed bovine semen
... to Hoechst 33342 intensity by flow cytometry, but the sorting procedure likely causes dam- age to sperm by laser and sorting stress ...(Hoechst 33342, FITC– PNA or SYBR-14/PI) sperms were ...
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Usnea filipendula Induces Apoptosis in Human Colon Cancer Cell Lines
... Lichens are complex organisms living in a symbiotic relationship with fungi and algae have recently received special interest in cancer research. The cytotoxic activities of Usnea filipendula Stirt. lichen extract was ...
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Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser
... From our data, we can conclude that for these specimens the photo-bleaching rate scales faster than linear with the excita- tion power, but not with an order (n) higher than 2. This is in contrast to the literature ...
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Visualizing the antivascular effect of bortezomib on the hypoxic tumor microenvironment
... or the right flank (for DCE MRI) of 6–8 weeks old female nude mice (NCr athymic nu/nu, National Cancer Institute Frederick Cancer Research Institute). Unless indicated otherwise, nude mice, bearing HT29–9HRE xenografts, ...
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Versatile inhibitory effects of the flavonoid-derived PI3K/Akt inhibitor, LY294002, on ATP-binding cassette transporters that characterize stem cells
... and Hoechst 33342 ...low Hoechst fluorescence and comprising about ...expels Hoechst but not rho123, while P-gp expels both Hoechst and ...
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Antitumor effects of a new compound TW9183 on human A549 lung cancer and SMMC 7721 hepatoma cell lines
... with Hoechst 33342 staining showed that TW9183 decreased viability of A549 and SMMC-7721 cells in a dose-dependent manner, especially for A549 cells, indicating a connection with its anti-proliferation ...
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Bioreducible cross linked core polymer micelles enhance in vitro activity of methotrexate in breast cancer cells
... The Hoechst 33342 stain (excitation/emission maxima = 350/461) was illuminated with a 100-watt high-pressure mercury plasma arc-discharge lamp (HBO ...
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Selective cytotoxicity of Pancratistatin-related natural Amaryllidaceae alkaloids: evaluation of the activity of two new compounds
... cell-permeable Hoechst 33342 dye; brightly stained and condensed nuclei were considered to be apoptotic while non-bright, smooth nuclei were considered healthy ...cell-permeable Hoechst 33342 ...
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Quantification of cellular viability by automated microscopy and flow cytometry
... Unless otherwise specified, experiments were performed in quadruplicate parallel instances, and data were analyzed with the R software (http://www.r-project.org/). The first-line flow cytometry data analysis was ...
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Tubulin binding dibenz[c,e]oxepines: Part 2 Structural variation and biological evaluation as tumour vasculature disrupting agents
... Representative images of DLD-1 human colon adenocarcinoma xenografts showing remaining functional vasculature using Hoechst 33342 staining, following a single intravenous dose of 15 or 2[r] ...
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Embryotoxicity assays for leached components from dental restorative materials
... Frequency histogram of ICM with different number of apoptotic cells evaluated by the nuclear staining, Hoechst 33342, of the embryo outgrowth cultured in control or composite-conditioned[r] ...
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EVOLUTION OF THE ALPHA-ESTERASE DUPLICATION WITHIN THE MONTANA SUBPHYLAD OF THE VIRILIS SPECIES GROUP OF DROSOPHILA
... Cytological studies, using aceto-orcein staining and Hoechst fluorescence of squashes of ganglion chromosomes, reveal acrocentric Y chromosomes for all six species of the mon[r] ...
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Prodigiosin induced apoptosis and inhibited proliferation in carcinoma hsc 2 cells
... The present study aims to investigate the anti-proliferative, apoptotic properties of prodigiosin, using a human oral squamous cell carcinoma HSC-2 cell line as a model system. HSC- 2 cells were cultured in the presence ...
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