Host-induced gene silencing
Post-transcriptional gene silencing and its implication to the asian soybean rust- a review article
... (Host Induced Gene Silencing) is used to down regulate fungal candidate genes in ...the silencing signal is formed in the plant but it mediates RNAi in the ...
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RNAi as an emerging approach to control Fusarium Head Blight disease and mycotoxin contamination in cereals
... targeted silencing of genes of interest in many eukaryotes. Host-induced gene silencing (HIGS) is a transgenic technology used to silence fungal genes in planta during attempted ...
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Baculovirus DNA Replication-Specific Expression Factors Trigger Apoptosis and Shutoff of Host Protein Synthesis during Infection
... of host protein synthesis is common among viruses (reviewed in references 11 and ...of host protein synthe- sis that parallels late and very late gene expression ...a host- mediated response ...
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Development of a gene silencing DNA vector derived from a broad host range geminivirus
... RNA silencing has been utilized to pro- duce phenocopies of knockout strains in various organ- ...induce silencing of the homologous gene ...the gene silencing response. In plants, ...
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Development of a Virus-Induced Gene Silencing System for Cotton and Its Application for Functional Genomics in Fiber.
... The family Geminiviridae consists of single-stranded (ss) DNA viruses with small monopartite or bipartite genomes. The name Geminivirus is derived from the distinctive shape of their virion as a twinned icosahedral ...
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Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated Nicotiana benthamiana extract
... Our goal was to identify a VIGS vector that can be used to assess functions of soybean genes active in specific tissue types, such as roots and seeds. The Apple latent spherical virus (ALSV)-based VIGS system was chosen ...
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Advances in the Application of Virus Induced Gene Silencing in Plants
... viral gene expression to DNA-A and DNA-B are almost identical in homologous areas containing cis-acting ...RNA silencing defenses, affect siRNA production, disturb microRNA biosynthesis, and cause abnormal ...
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Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance
... by host defenses during the early asymp- tomatic infection phase, and how it subsequently triggers host pro- grammed cell death during the necrotrophic phase of ...ing gene function in the fungus on ...
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Tomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique
... Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense ...containing host genes inserted in the viral genome, VIGS achieves the RNA silencing ...
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Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants
... virus- induced gene silencing (VIGS) assays, partial coding sequences of NbHDA6 (C-terminal 330 to 430 amino acids of NbHDA6, which is not conserved in the HDAC family) and GUS were amplified and ...
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Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system
... There are two other relatively easy and inexpensive methods for inoculating begomovirus vectors: DNA abrasion and agroinoculation. DNA abrasion involves the use of an abrasive (carborundum or ground glass) to introduce a ...
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A rapid virus induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease
... CMG-infected host to healthy plants [12–14]; b) delivery of DNA genomes as infectious clones via microparticle bombardment [15–17]; c) Agrobacter- ium-mediated inoculation of plants with cloned infectious DNA ...
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Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
... In this study, five PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;1, PsPIP2;2, and PsPIP2;3) were identified and cloned in Pisum sativum. In the preliminary trials, our quantitative real-time PCR (q-RT-PCR) results revealed ...
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RNA interference-mediated silencing of speckle-type POZ protein promotes apoptosis of renal cell cancer cells
... of silencing the speckle-type POZ protein (SPOP) gene on renal cell cancer (RCC) cells and to explore its possible ...The silencing efficiency was monitored by quantitative real-time polymerase chain ...
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A REVIEW ON GENE SILENCING
... • These catalytic RNA molecules bind to a specific site and attack the neighboring phosphate in the RNA backbone with their 2’ oxygen, which acts as a nucleophile, resulting in the formation of cleaved products.This ...
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Analysis of Begomoviruses-Mediated Gene Silencing
... AR1 gene and introduction of a polylinker was accomplished by replacing the AR1 sequences with two PCR generated ...AR1 gene replaced with a polylinker to facilitate insertion of foreign DNA ...AR1 ...
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Geminivirus-induced Gene Silencing as a Method to Determine the Role of Essential Cell Cycle Genes in Plant Development
... of silencing that occured when a mutant AL1-containing TGMV A component was used in combination with ...diffusible silencing signal might not be so ...geminivirus gene expression (Kong et ...for ...
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ATP2B1 gene Silencing Increases Insulin Sensitivity through Facilitating Akt Activation via the Ca2+/calmodulin Signaling Pathway and Ca2+-associated eNOS Activation in Endothelial Cells
... ATP2B1 gene KO mice ...ATP2B1 gene knockdown-induced hypertension ...ATP2B1 gene, was significantly associated with T2DM in two community-based cohorts from the Korea Association Resource ...
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Is selenoprotein K required for Borrelia burgdorferi infection within the tick vector Ixodes scapularis?
... Quantification of B. burgdorferi in I. scapularis tissues The principle of quantification of B. burgdorferi load was followed from our studies established to quantify the load of the spotted fever group rickettsia, ...
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FOXP3 is a novel transcriptional repressor for the breast cancer oncogene SKP2
... Silencing of FOXP3 resulted in upregulation of SKP2 in primary HMEC. FOXP3 was silenced in HMEC by using siRNA. FOXP3 and SKP2 transcripts were quantified by real-time PCR. The RNA inputs were normalized against ...
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