Housekeeping Gene
Variable genome evolution in fungi after transposon-mediated amplification of a housekeeping gene
... H3 gene in a sea of inactivated, partial, RIP-affected ...original gene was protected from RIP, possibly be- cause the duplicated fragment was too small to trigger recognition of the original ...a ...
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Comparison of MALDI TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates
... and housekeeping gene sequencing (gyrB, ...sequencing, housekeeping gene sequencing with phylogenetic analysis, and MALDI-TOF ...Overall, housekeeping gene sequencing with ...
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Housekeeping gene expression stability in reproductive tissues after mitogen stimulation
... Background: Intrauterine infection during pregnancy can trigger a local inflammatory response leading to several complications, such as preterm labor. Many studies have used in vitro and in vivo models employing mitogens ...
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Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage
... K63-linked polyubiquitination process is primarily involved in stress responses, within which the E2 com- plex plays a critical role. It is reasonable to assume that the Ubc13-Uev activity is tightly regulated in ...
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ProNGF p75NTR axis plays a proinflammatory role in inflamed joints: a novel pathogenic mechanism in chronic arthritis
... Figure 1 p75NTR, TrkA and sortilin expression levels in patients with JIA. (A) mRNA expression levels of TrkA, p75NTR and sortilin in freshly isolated mononuclear cells from peripheral blood of healthy children (CTRL ...
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Ion Torrent Personal Genome Machine Sequencing for Genomic Typing of Neisseria meningitidis for Rapid Determination of Multiple Layers of Typing Information
... additional housekeeping gene loci (extended MLST [eMLST] [9]), complete porA gene, partial and complete porin B (porB) gene, vaccine antigen gene fhbp (6), and antimicrobial re- ...
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Novel Method To Identify Source Associated Phylogenetic Clustering Shows that Listeria monocytogenes Includes Niche Adapted Clonal Groups with Distinct Ecological Preferences
... In order to allow the unbiased identification of phylogenetic clades that are associated with different sources populations, rather than using clades defined a priori (e.g., the major L. monocytogenes genetic lineages), ...
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<p>Induction of Robust Immune Responses by CpG-ODN-Loaded Hollow Polymeric Nanoparticles for Antiviral and Vaccine Applications in Chickens</p>
... Since several immune genes were observed to be stimulated by CpG-NP treatment in chBMDC, we sought to know if this activa- tion can contribute to antiviral immunity. Based on the assay procedure illustrated in Figure 5A, ...
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Multilocus Sequence Typing of Streptococcus uberis Provides Sensitive and Epidemiologically Relevant Subtype Information and Reveals Positive Selection in the Virulence Gene pauA
... sequences. Housekeeping genes, virulence genes, and genes encoding vaccine targets were included in our MLST scheme to allow studies on population genetics as well as the evolution of virulence and vaccine target ...
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Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage
... as housekeeping genes in cartilage studies in various ...a housekeeping gene in studies of human, bovine, porcine, and caprine articular cartilage, including both normal and osteoarth- ritic (OA) ...
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Quantitative profiling of housekeeping and Epstein Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray
... high-throughput gene-profiling technologies, such as microarray and ...eight housekeeping genes with similar transcription levels in the five BL cell lines tested and, most importantly, in the marmoset LCL ...
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Genetic Diversity of Neisseria gonorrhoeae Housekeeping Genes
... Typing of bacterial isolates can be used for clinical purposes to establish strain-specific correlates of microbial pathogenesis, for epidemiological purposes to investigate the endemic and epidemic spread of organisms, ...
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BART Seq: cost effective massively parallelized targeted sequencing for genomics, transcriptomics, and single cell analysis
... and housekeeping gene transcripts (five exon spanning), as well as four exogenous RNA spike-in molecules, which we validated by nested qPCR (Table 1, Additional file 7: Figure S2a, b, Additional file 2: ...
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Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
... other housekeeping genes investigated, only TBP and B2M showed a stable and constant expres- sion irrespective of cellular plating density, oxygen partial pressure and FSH ...stable housekeeping gene ...
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An Atypical Clostridium Strain Related to the Clostridium botulinum Group III Strain Isolated from a Human Blood Culture
... Anaerobic bacteria are responsible for various types of infec- tions, including bacteremia. Bloodstream infections with anaer- obes (1 to 17% of positive blood cultures) are mainly due to Gram-negative bacilli. ...
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Human microglia and astrocytes constitutively express the neurokinin-1 receptor and functionally respond to substance P
... Panel b At 2 h, protein expression of fNK-1R, the truncated NK-1R isoform (tNK-1R), and the housekeeping gene product β -actin, was determined by immunoblot analysis for each of the four[r] ...
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Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen
... of housekeeping gene sequences for RNA polymerase subunit B (rpoB) and gyrase A (gyrA) identified sequence similarity within the Bacillus genus, confirmed by 16S rRNA gene sequence similarity which ...
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Inhibition of oncogene-induced inflammatory chemokines using a farnesyltransferase inhibitor
... the housekeeping gene G3pdh, the pro- inflammatory genes Ccl2 and Cxcl1, as well as the onco- gene RP3 was determined using ...the gene expres- sion of G3pdh, Ccl2, Cxcl1, and RP3 in PC Cl3 ...
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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... Design of MB and primers. The PCR primers and MB were designed based on the sequence of the CCR5 gene (GenBank accession no. AF011539) by using Primer Express software (PE Biosystems) and were synthesized by ...
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Nutlin-3 inhibits androgen receptor-driven c-FLIP expression, resulting in apoptosis of prostate cancer cells
... Inhibition of androgen receptor (AR) signalling represents the conventional medical management of prostate cancer. Ultimately this treatment fails because tumors develop an incurable, castrate resistant phenotype, ...
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