HPV 18 E6 and E7
A novel HPV 16 L1 based chimeric virus like particle containing E6 and E7 seroreactive epitopes permits highly specific detection of antibodies in patients with CIN 1 and HPV 16 infection
... ethanol. HPV DNA was amplified using the general primers MY09 (5’-CGTCCMARRGGAWACTG- ATC-3’) and MY11 (5’-GCMCAGGGWCATAAYAA- TGG-3’) [41], which amplified a conserved 450-bp fragment from the L1 ...of ...
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Inactivation of the Human Papillomavirus E6 or E7 Gene in Cervical Carcinoma Cells by Using a Bacterial CRISPR/Cas RNA-Guided Endonuclease
... endogenous HPV-18 E6 and E7 genes integrated into the HeLa cell ...on E6 sgRNA1 and E7 ...an E6- or E7-specific ...the HPV-18 genome containing either ...
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PDZRN3/LNX3 Is a Novel Target of Human Papillomavirus Type 16 (HPV-16) and HPV-18 E6
... by E6, HeLa cells were transfected with siRNA for luciferase or siRNA for HPV18E6/E7 and, after 48 h, subjected to immunoflu- orescence analysis, probing for PDZRN3 and p53 as a positive control for ...
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Suppression of cellular proliferation by the papillomavirus E2 protein.
... oncogenes, E6 and ...an HPV type 18 (HPV-18)-positive cervical carcinoma cell line results in growth ...the HPV-16 and HPV-18 E2 proteins share with BPV-1 E2-TA the ...
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Single-tube multiplex PCR using type-specific E6/E7 primers and capillary electrophoresis genotypes 21 human papillomaviruses in neoplasia
... integrated HPV DNA would remain in the host cell and this would include the obligate E6/E7 oncogenes ...the E7 oncogene showed greater analytical sensitivity than PCR with broad-spectrum ...
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E6^E7, a Novel Splice Isoform Protein of Human Papillomavirus 16, Stabilizes Viral E6 and E7 Oncoproteins via HSP90 and GRP78
... of E6^E7 pre-mRNA by 68%, but introduc- tion of the same mutation at nt 718 reduced the splicing efficiency by only 30% (lanes ...of E6^E7 pre- ...HPV16 E6^E7 pre-mRNA takes ...
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Immortalization and altered differentiation of human keratinocytes in vitro by the E6 and E7 open reading frames of human papillomavirus type 18.
... In the present study, we examined the intact E6-E7 region of HPV-18, as well as individual E6 and E7 gene products, for the abilities to immortalize keratinocytes and alter their differe[r] ...
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Squamous epithelial hyperplasia and carcinoma in mice transgenic for the human papillomavirus type 16 E7 oncogene.
... to HPV-16 sequences from nucleotides (nt) 79 to 883 encompassing the tandemly positioned E6 and E7 ...the E6 and E7 open reading frames (ORFs), the presence of the translation ...
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Involvement of aryl hydrocarbon receptor signaling in the development of small cell lung cancer induced by HPV E6/E7 oncoproteins
... that HPV DNA could exist in peripheral blood mononuclear cells (PBMCs) of patients with geni- tal HPV 16 infection and with cervical ...that HPV infec- tion may also play a role in the development of ...
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Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Biopsy Samples from Cervical Cancer Patients in North India
... particularly HPV types 16 and 18 (HPV-16 and HPV-18, respectively), play a cardinal role in the etiology of cervical ...type, HPV-16, shows intratypic sequence variants that are ...
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Cytology and Human Papillomavirus Testing 6 to 12 Months after ASCUS or LSIL Cytology in Organized Screening To Predict High Grade Cervical Neoplasia between Screening Rounds
... PreTect HPV-Proofer 6 to 12 months after screening-detected ASCUS/LSIL detected ...PreTect HPV-Proofer in another study, with a multiple-test comparison similar to ours but with less than 1 year of ...
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THE CARCINOGENIC POTENTIAL OF E6 & E7 GENES OF HIGH RISK HPV COMPARED WITH E6, E7 GENES OF LOW RISK HPV IN HUMAN CERVICAL CANCER: A REVIEW
... genital HPV infections and cervical cancer was first demonstrated in the early 1980s by Harold Zur Hausen, a German ...recognized HPV as an important cause of cervical ...30 HPV types that are spread ...
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Human papilloma virus E6/E7 messenger RNA as a biomarker for detecting the risk evaluation of cervical cancer progression: overview of recent clinical trials
... and HPV DNA detection was performed by consensus Gp5+/6+ PCR and HPV type specific PCR for HPV types 16, 18, 31, 33 and ...PreTect HPV-proofer test was used for HPV mRNA ...for ...
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Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile
... reporting HPV presence in ...both, HPV-16 and -18 were able to immortalize and change the proliferative properties of epithelial breast cells ...of HPV and EBV in BC from Chile, an ...
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Glycogene expression profiles based on microarray data from cervical carcinoma HeLa cells with partially silenced E6 and E7 HPV oncogenes
... infections. HPV-16 is the most prevalent high-risk HPV type, followed by HPV-18 and HPV-31 ...The HPV-18 type is the most prevalent geno- type in cervical adenocarcinoma ...
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Natural variants of the human papillomavirus type 16 E6 protein differ in their abilities to alter keratinocyte differentiation and to induce p53 degradation.
... the E6 ORF or the E6 and E7 ORFs as a unit were subcloned from the intermediate vectors used for sequence determination into a derivative of pSG5 (18) which expresses inserts under the control ...
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Genetic variability in E6 and E7 oncogenes of human papillomavirus Type 16 from Congolese cervical cancer isolates
... twenty HPV-16 isolates was analyzed by amplifying the E6 and E7 genes using type-specific primers PCR and direct ...the HPV-16 GenBank reference ...revealed 18 and 4 nucleotide changes ...
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E6/E7 Expression of Human Papillomavirus Type 20 (HPV-20) and HPV-27 Influences Proliferation and Differentiation of the Skin in UV-Irradiated SKH-hr1 Transgenic Mice
... K10 HPV-20 E6/E7 and K10 HPV-27 E6/E7 transgenes con- tained the respective E6 and E7 open reading frames under the control of the keratin 10 promoter and were ...
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<p>An efficient method that combines the ThinPrep cytologic test with E6/E7 mRNA testing for cervical cancer screening</p>
... ned HPV 16/18 as positive, and its speci fi city was dramatically increased from ...simultaneously. HPV 16/18 with a Youden index of ...and E6/E7 mRNA were ...
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Simultaneous Amplification and Identification of 25 Human Papillomavirus Types with Templex Technology
... Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 ...for ...
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