HPV DNA detection using PGMY09/11 primers:
Enhanced Detection and Typing of Human Papillomavirus (HPV) DNA in Anogenital Samples with PGMY Primers and the Linear Array HPV Genotyping Test
... LA- HPV to detect HPVs compared with ...PGMY primers were identi- cal for both systems, making it less likely that the excess detection rate for LA-HPV was due to nonspecific hybridiza- tion ...
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HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes
... oligonucleotides primers used, the number of base pairs of the product amplified by PCR, performance of DNA polymerase used in the reaction, DNA-HPV type spectrum amplified, and the ability to ...
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CIN2+ detection of the HPV DNA Array genotyping assay in comparison with the Cobas 4800 HPV test and cytology
... specific HPV types, a high sensitivity for detection of HPV 16 was observed (>90%, k = ...for HPV 18 detection (54.2%, κ = 0.681, p < 0.05), and for detection of other 12 ...
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Comparative analysis of three methods for HPV DNA detection in cervical samples.
... words: HPV detection; HC2; L1-Nested-PCR, ...risk HPV infection is considered to play a central role in cervical ...carcinogenesis. HPV DNA testing has shown to be a very useful tool ...
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HPV DNA-Chip. PapilloCheck HPV-Screening. DNA-Chip for the genotyping of 24 types of genital HPV
... the detection of a fragment of the E1 gene of 24 different ...human DNA are extracted from a cervical smear ...of HPV specific primers by polymerase chain reaction ...
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Good concordance of HPV detection between cervico vaginal self samples and general practitioner collected samples using the Cobas 4800 HPV DNA test
... since DNA is generally considered to be stable at -80 °C, we assume that this has not significantly affected the results and con- clusions of this ...concurrent HPV infection; thus, histo- logical results ...
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Alpha, Beta, gamma human PapillomaViruses (HPV) detection with a different sets of primers in oropharyngeal swabs, anal and cervical samples
... ɣ HPV types and OSCC ...β HPV types in the de- velopment of SCC in immunocompetent individuals ...β HPV types are able to target cellular proteins, such as p53 and pRb, and to deregulate fundamental ...
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Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
... target DNA. In seven samples, an HPV type was detected only by the standard consensus PCR assay and not by the PGMY-line ...PGMY primers or a low viral load. Due to the genetic diversity of ...
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Detection of genetic variability in Chrysanthemum (Dendranthema grandiflora T.) using ISSR primers
... molecular DNA markers has lately gained lot of attention (Guena et ...breeding. DNA molecular marker technology, which are based on sequence variation of specific genomic regions, provide powerful tools for ...
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Detection of HPV DNA in paraffin embedded cervical samples: a comparison of four genotyping methods
... Reverse primers contain a biotin label at the 5’ end, enabling capture of the reverse strand onto streptavidin- coated microtiter ...of HPV genotypes. This method is designated an HPV DNA ...
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Comparison of Real Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection
... the DNA extraction technique was held as a constant, with DNA isolated from swab specimens using the Qiagen Spin blood kit, the preferred process for use with multiplex HPV ...multiplex ...
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PCR-based detection of Plasmodium falciparum in saliva using mitochondrial cox3 and varATS primers
... cox3 primers target a gene specific to Plasmo- dium , thereby eliminating the risk of non-specific amp- lification of human ...varATS primers have the advantage of being used in a single-step PCR, thereby ...
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The use of HPV DNA testing combined with Pap smear in detection of pre invasive disease of the cervix
... analyzed using IBM SPSS software package version ...tested using Chi-square ...conducted using Fisher’s Exact test or Monte Carlo ...done using independent t-test while for abnormally ...
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Options in human papillomavirus (HPV) detection for cervical cancer screening: comparison between full genotyping and a rapid qualitative HPV-DNA assay in Ghana.
... extraction and require very limited technical knowledge to be performed. While careHPV has been evaluated in some settings, this research presents the first such evalu- ation done among women including HIV seropositive ...
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Virus detection and identification using random multiplex (RT) PCR with 3' locked random primers
... 5'-VVVVVVVVAA-3' Primers Enable Random Multiplex Amplification of Plasmid DNA We next designed a mixture of random primers that would enable the priming and amplification of all DNA or cDNA ...
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Assessment of MagNA Pure LC Extraction System for Detection of Human Papillomavirus (HPV) DNA in PreservCyt Samples by the Roche AMPLICOR and LINEAR ARRAY HPV Tests
... AMPLICOR HPV test when DNA extracted by the Roche MP is used as opposed to the recommended AmpliLute method, and thus the Roche MP method could substitute for these ...MP using a DNA-I kit of ...
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Introduction of primary screening using high-risk HPV DNA detection in the Dutch cervical cancer screening programme: a population-based cohort study
... reported using high-quality data with extended ...efficient. Detection of CIN2+ lesions was higher in the hrHPV-based programme at the cost of more unnecessary ...
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Comparison of Onclarity Human Papillomavirus (HPV) Assay with Hybrid Capture II HPV DNA Assay for Detection of Cervical Intraepithelial Neoplasia Grade 2 and 3 Lesions
... negative HPV test result when systems based on L1 consensus primers PCR are used (22, ...guished: HPV genotypes 16, 18, 45, 31, 51, 52, 33, 58, 56, 59, 66, 35, 39, and 68 can be identified in single ...
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Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... genomic DNA from most M. ulcerans strains using a smaller number of cycles than that required with the conventional ...PCR-based detection; however, the in- crease in sensitivity varied depending on ...
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Validation of a Human Papillomavirus (HPV) DNA Cervical Screening Test That Provides Expanded HPV Typing
... in detection between tests at the positive/ negative level, other factors are likely to become more meaningful in the choice by a laboratory or clinical center in using one test or the ...high-risk ...
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