huh 5-2 cells
In vitro antiviral activity of some novel isatin derivatives against HCV and SARS-CoV viruses
... DC). Cells were seeded at a density of 7000 cells per well in 96 well view plate TM (Packard, CA) in medium containing the same compounds as described above, except for ...G418. Cells were allowed to ...
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Hepatitis C Virus Genotype 1a Growth and Induction of Autophagy
... The accumulation of autophagy gene products during cell death, and their relationship with members of the Bcl-2 family, is a promising area for better understanding how autophagy is controlled during cell death. ...
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Diverse Effects of Cyclosporine on Hepatitis C Virus Strain Replication
... with cells transfected with the NN strain (NNC#2 cells [NN/ 1b/FL]) ...in Huh-7, NNC#2 (NN/1b/FL), and MH-14 (NN/1b/SG) cells analyzed by immunoblot analysis are ...in ...
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<p>Inhibition of Aurora Kinase A by Alisertib Reduces Cell Proliferation and Induces Apoptosis and Autophagy in HuH-6 Human Hepatoblastoma Cells</p>
... Although pediatric liver tumors are rare, hepatoblastoma (HB) is the fastest rising tumor and the most common hepatic malignant tumor globally in children under the age of 5. 1,2 The incidence of HB in males is ...
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Persistent Hepatitis C Virus Infection In Vitro: Coevolution of Virus and Host
... parental Huh-7.5.1 and Huh-7/scr cells. The cells were stained with a mouse anti-human CD81 antibody followed by a phycoerythrin-conjugated secondary ...R3 cells 3 days after infection. ...
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Contrasting Roles of Mitogen-Activated Protein Kinases in Cellular Entry and Replication of Hepatitis C Virus: MKNK1 Facilitates Cell Entry
... (A) Cells were pretreated with AS601245 (0 to ...HJ3-5-infected cells treated as described for panel ...(Top) Huh-7 cells were inoculated with genotype 1a H77S.2 virus (33) in ...
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Hepatitis C Virus Induces Proteolytic Cleavage of Sterol Regulatory Element Binding Proteins and Stimulates Their Phosphorylation via Oxidative Stress
... HCV-infected cells, cel- lular lysates from cells infected with HCV were subjected to SDS-PAGE followed by Western blot ...assays. Huh-7 cells were transfected with 500 ng of SRE-responsive ...
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Identification of Piperazinylbenzenesulfonamides as New Inhibitors of Claudin-1 Trafficking and Hepatitis C Virus Entry
... (A) Huh-7 cells were treated for 3 h with SB258585 at different concentrations, concomitant with HCVpp JFH1 or RD114pp ...postinfection, cells were lysed and luciferase activity measured in order to ...
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New Mechanism of Hepatic Fibrogenesis: Hepatitis C Virus Infection Induces Transforming Growth Factor β1 Production through Glucose-Regulated Protein 94
... control Huh-7 cells ...liver cells of HCV patients express high levels of TGF-  1 ...(lanes 2 to 6) or without (lane 1) infection of ...
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Mathematical Modeling of Subgenomic Hepatitis C Virus Replication in Huh-7 Cells
... HCV polyprotein (⬃2,000 amino acids) is translated at a mean rate of 10 (range, 5.4 to 14.4) polyproteins per h per ribosome. Wang et al. (59) have shown that at least eight ribosomes were present on an efficient ...
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Inhibitory Effects of Bile Acids and Synthetic Farnesoid X Receptor Agonists on Rotavirus Replication
... MA104, Huh-7, and Caco-2 cell lines were maintained in minimum essential medium (MEM) or Dulbecco’s MEM (DMEM) containing 5% fetal bovine serum and antibiotics (penicillin and strep- ...
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Original Article Overexpression of DDX3 inhibits cellular activity in hepatocellular carcinoma cells
... lines Huh-7 cells using Trizol reagent (Invi- trogen, CA, ...forward 5’-CAAGAGTCAGCAACTGCAG- AAG-3’ and reverse 5’-TAAGGTCGCAATCCAA- ...forward 5’- GTGGACATGCGCTAAGAC-3’, and reverse ...
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Expression of an IRF 3 fusion protein and mouse estrogen receptor, inhibits hepatitis C viral replication in RIG I deficient Huh 7 5 cells
... in Huh 7.5 cells treated with 4-HT. Lane 2 shows both IRF-3 and IRF- 3ER (monomer) (90 kd) in ...Huh7.5-IRF3ER cells without 4-HT ...Huh7.5-IRF3ER cells induces IRF-3ER fusion protein ...
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Cell-Free Replication of the Hepatitis C Virus Subgenomic Replicon
... from Huh-7 cells selected in the presence of G418 after RNA trans- fection with a subgenomic HCV ...These cells stably express the HCV replicon at a high efficiency and contain adaptive mutations ...
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New Insights into the Understanding of Hepatitis C Virus Entry and Cell-to-Cell Transmission by Using the Ionophore Monensin A
... purpose, Huh-7 cells were infected with HCVcc for 2 h and then cultured with neutralizing anti-E2 antibody (3/11) in the presence or absence of monensin ...of cells per ...of cells per ...
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Proteome Analysis of Liver Cells Expressing a Full-Length Hepatitis C Virus (HCV) Replicon and Biopsy Specimens of Posttransplantation Liver from HCV-Infected Patients
... In recent genomic analyses of liver biopsies from acutely infected chimpanzees and an in vitro subgenomic replicon model, the accumulation of free fatty acids associated with transcriptional changes in host genes ...
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Response of Hepatitis C Virus to Long-Term Passage in the Presence of Alpha Interferon: Multiple Mutations and a Common Phenotype
... and 56 h later cells were treated with 2 IU/ml (for p0 and p45 populations) and 12 IU/ml (for p100 populations) of IFN-␣ for 16 h. Under these conditions, at the time of labeling at least 90% of the ...
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Prohibitin is overexpressed in Huh 7 HCV and Huh 7 5 HCV cells harboring in vitro transcribed full length hepatitis C virus RNA
... hepatoma cells transfected with full- length HCV RNA compared with the control ...in Huh-7-HCV cells was 2.09 times higher than that in Huh-7 control cells; while the prohibitin ...
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ATF3 inhibits the tumorigenesis and progression of hepatocellular carcinoma cells via upregulation of CYR61 expression
... cancer cells encountered DNA damage, ATF3 and p53 could synergistically act on the promoter of the DR5 gene to eventually promote TRAIL-mediated apoptosis ...
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Characterization of Lassa Virus Cell Entry and Neutralization with Lassa Virus Pseudoparticles
... pH 5 is specific, because the pH-insensitive MLV-A and RD114 GPs can withstand such treatment without changes to their respective fusogenic behaviors and particle ...target cells (C). Neutralizations with ...
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