Human colonic epithelial cells
Differential expression of Aquaporin 8 in human colonic epithelial cells and colorectal tumors
... 10 µ g total RNA from 3 normal colonic tissues, 2 HNPCC carcinomas, 3 sporadic colon carcinomas and 2 colon cancer cell lines, was separated by size using electro- phoresis on 1.5% denaturing agarose gel. The RNA ...
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Expression of functional CXCR4 chemokine receptors on human colonic epithelial cells
... in human colonic epithelium, HT-29 cells were examined by RT-PCR for the expression of the chemokine receptors for lymphotactin, fractalkine, CCR1-10, and ...HT-29 cells did not express mRNA ...
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The antifungal antibiotic, clotrimazole, inhibits Cl secretion by polarized monolayers of human colonic epithelial cells
... intestinal crypt epithelial cells also participate in secretagogue-stimulated Cl- secretion. We examined the ability of CLT to block intestinal Cl- secretion by inhibition of K+ transport. Cl- secretion was ...
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Bile acid: a potential inducer of colon cancer stem cells
... normal human colonic epithelial cells and that this induction is greatly attenuated when M3R is downregulated in colonic epithelial cells further supports a role for M3R ...
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Blastocystis ratti Contains Cysteine Proteases That Mediate Interleukin-8 Response from Human Intestinal Epithelial Cells in an NF-κB-Dependent Manner
... Blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and a wide range of animals. Evidence accumulated over the last decade suggests association of Blastocystis with gastrointestinal ...
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Shigella flexneri Adherence Factor Expression in In Vivo-Like Conditions
... to colonic HT-29 ...upon colonic transition following the loss of the bile salts signal, and the subsequently induced infection (31, ...a human intestinal organoid-derived epithelial monolayer ...
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Strongyloides spp in cats: a review of the literature and the first report of zoonotic Strongyloides stercoralis in colonic epithelial nodular hyperplasia in cats
... B cells to initiate the immune response, in response to local anti- ...of cells that stained positive for CD3 (T-cells) and cells that stained positive for CD79 (B-cells), whereas the ...
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Sirtuin-6 Preserves R-spondin-1 Expression and increases Resistance of intestinal Epithelium to injury in Mice
... the cells were main- tained in RPMI 1640 medium (Thermo Fisher Scientific) containing 5% heat- inactivated fetal bovine serum (Thermo Fisher Scientific), 5 U/mL mouse inter- feron-γ (IFN-γ; PeproTech, Rocky Hill, ...
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Bitter melon protects against ER stress in LS174T colonic epithelial cells
... The anti-oxidant activity of BME was assessed by its effect on the copper-induced oxidation of human serum. Fasting plasma was collected from 6 healthy individuals (three men and three women) and isolated serum ...
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Influence of NADPH oxidase on inflammatory response in primary intestinal epithelial cells in patients with ulcerative colitis
... non-phagocytic cells from damage in- duced by bacterial products and can reduce damage in the colon tissues ...colon epithelial cell ...LPS-treated colonic epithelial ...in human ...
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Ursodeoxycholic acid attenuates colonic epithelial secretory function
... increased colonic delivery in conditions of bile acid malabsorption causes diarrhoea (Chadwick et ...on colonic epithelial ...cultured cells and in pharmacologically denervated human ...
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A Novel Role for Programmed Cell Death Receptor Ligand-1 in Sepsis-Induced Intestinal Dysfunction
... intestinal epithelial cells (IECs) has a role in sepsis-induced intestinal barrier ...nonseptic colonic samples (assigned by pathology report) were immunohistochemically stained for PD-L1 in a ...
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Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells
... Calu-3 cells, respectively, resulting in either reduced or completely inhibited viral replication (9, 13), suggesting the exis- tence of some variations in the effectiveness of prior IFN treat- ment of infections ...
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Overexpression and cytoplasmic localization of caspase-6 is associated with lamin A degradation in set of ovarian cancers
... Cells were cultured in six well dishes in respective media. Cells were washed with ice cold PBS and lysate with 200 μl RIPA buffer (Santa Cruz Biotechnology) in ice for 30 min. Aliquot of 2 μl was used for ...
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Weidlich, Simon (2017): Expression of Interleukin-37 in paediatric chronic inflammatory bowel disease. Dissertation, LMU München: Medizinische Fakultät
... ab was applied for 20’, then the incubation buffer was poured off. For Abnova IL-37 Ab, endogenous biotin was blocked using Biotin Blocking System (Dako). Polyclonal goat anti human primary Ab against IL-37 ...
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Basal cytokeratins and their relationship to the cellular origin and functional classification of breast cancer
... stem cells and their relationship to kinetics in the normal breast was excellently reviewed by Behbod and Rosen ...normal human breast and on what we know about the origin of breast cancer from ...
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Fibroblasts modulate intestinal secretory responses to inflammatory mediators
... Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal ...T84 colonic epithelial cells, measured as ...
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Adherence of skin bacteria to human epithelial cells
... Aerobic and anaerobic bacteria isolated from human axillae were tested for their capacity to adhere to buccal epithelial cells, immortalized human epithelial HEp-2 cells, and undifferent[r] ...
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Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes
... intestinal epithelial cells is thought to be important in regulating immune response in the intestinal mucosa, but conclusive evidence is ...in human intestinal mucosa by combined reverse ...
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Species-specific enhancement of enterohemorrhagic E. coli pathogenesis mediated by microbiome metabolites
... vitro human disease models that can enable direct investigation into the cellular and molecular basis of human host-micro- biome-pathogen ...The human Colon Chip model of EHEC infection described ...
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