human corneal epithelial cell
Expression of Beta Defensin Genes in Frozen Thawed and Cultured Immortalised Human Corneal Epithelial Cell Line
... immortalised human corneal epithelial cell line (HCE-2) has not been ...that corneal epithelium constantly produce hBD-1 and hBD-3, which presumably provide the baseline defence against ...
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Comparison of an immortalized human corneal epithelial cell line with Vero cells in the isolation of Herpes simplex virus-1 for the laboratory diagnosis of Herpes simplex keratitis
... of corneal blindness, is a sight threatening ocular infection often re- quiring a specific and prompt laboratory diagnosis ...from corneal scrapings, may be attributed to the sensi- tivity of the ...
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The pathogenesis of Acanthamoeba infections: current status and future implications
... Acanthamoeba-mediated human corneal epithelial cell cytotoxicity using ecto-ATPase inhibitor, suramin (P2 receptor antagonist), clearly demonstrating that ecto-ATPases play an important role ...
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Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells
... another cell type without dedifferentiation to a pluripotent state by introducing tissue specific transcription factors ...the corneal epithelial lineage no protocol exists so ...limbal ...
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Cellular therapy of corneal epithelial defect by adipose mesenchymal stem cell-derived epithelial progenitors
... closed corneal surface reduced the exposure of MET-Epi cells to air-liquid interphase that could have hindered the epithelial cell polarization and differenti- ation ...the corneal ...
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The PACAP-derived peptide MPAPO facilitates corneal wound healing by promoting corneal epithelial cell proliferation and trigeminal ganglion cell axon regeneration
... regeneration [46]. In nerve cells, PACAP activates related signaling pathways through the PAC1 receptor, promotes neuronal survival, inhibits neuronal cell apoptosis, and protects neurons. PAC1 activates the cAMP ...
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Transient receptor potential (TRP) gene superfamily encoding cation channels
... of corneal epithelial wound healing following injury, as well as inducing inflam- mation through increases in the expression of inter- leukin (IL)-6 and IL-8, which are proinflammatory and chemoattractant ...
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A mathematical approach to human pterygium shape
... the corneal epithelium is to be maintained, cell loss must be balanced by cell replacement (X + Y = Z, where X stands for the proliferation of basal epithelial cells, Y stands for the ...
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Establishment and Characterization of an Air-Liquid Canine Corneal Organ Culture Model To Study Acute Herpes Keratitis
... in corneal cellular gene expression upon CHV-1 ...in human corneal epithelial cells upon in vitro infection with HSV-1, and an accumulation of this cytokine was found in murine cor- neas using ...
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The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells
... fumigatus hyphae for 15, 30, 45, 60 min in 6-well plates and 4, 8, 16 h in 12-well plates. After treatment of 4– 16 h, cells were lysed for total RNA extraction and mRNA detection. After treatment of 0–60 min, ...
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Azithromycin: assessment of intrinsic cytotoxic effects on corneal epithelial cell cultures
... incubated corneal epithelial cells for 15 minutes or 6 hours with preservative-free netilmicin or levofloxacin and different concentrations of unpreserved ...injured corneal epithelial cells ...
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Cytogenetic characterization and H-ras associated transformation of immortalized human mammary epithelial cells
... SKY [22] was used to identify or confirm the G-band kary- otype assignments. Briefly, SKY is a form of fluorescence in situ hybridization (FISH) which utilizes a mixture of "whole chromosome paint" DNA probes to ...
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Comparison of single-step reverse transepithelial all-surface laser ablation (ASLA) to alcohol-assisted photorefractive keratectomy
... Sixty eyes of 30 myopic patients (19 male and 11 female) undergoing excimer photorefractive keratectomy were included. The average age was 29 years (SD: 9; range: 17–46). Preoperative mean UDVA was 0.01 Snellen fraction ...
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Ziegelaar, Brian (2004): Tissue engineering of a tracheal substitute. Dissertation, LMU München: Medizinische Fakultät
... The cell density in mature cartilage tissue is low and unlike the respiratory epithelium, (particularly the ciliated and goblet cells), the functional aspects of mature cartilage are derived more from the ECM than ...
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Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
... using a scalpel blade. The tissue was then incubated in 1.2 U/ml dispase II for 40 min at 37 °C. Subsequently, the tissue was incubated in 2 mg/ml collagenase and 0.5 mg/ml hyaluronidase overnight at 37 °C on a shaker. ...
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Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction
... limbal epithelial transplantation (CLET) in the clinical success ...entire corneal surface is covered with transplanted cultivated corneal epithelium (B) confirmed by fluorescein staining, with no ...
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Dimeric procyanidins as modulators of airway inflammation in the context of allergic asthma : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy (PhD) in Human Physiology at Massey University, Manawatū, Palmerston North, New Zealand
... Bioavailability is defined as the proportion of compounds that are absorbed and enter the blood stream so they can be distributed and metabolized within body organs. The term bioaccessible is used to refer to a compound ...
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Human basonuclin 2 up-regulates a cascade set of interferon-stimulated genes with anti-cancerous properties in a lung cancer model
... adenocarcinoma cell line A549 compared to the human normal bronchial epithelial cell line BEAS-2B, as well as in lung tumor tissue compared to non-tumor ...
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Uptake of DNA by cancer cells without a transfection reagent
... immortalized human liver cells, which are nontumo- rigenic and AFP expression negative, and were cultured in BEBM medium (Lonza/Clonetics) supplemented with various growth ...cancer cell lines Huh7 and ...
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Release of Macrophage Migration Inhibitory Factor and CXCL8/Interleukin-8 from Lung Epithelial Cells Rendered Necrotic by Influenza A Virus Infection
... contrast, cell lines U1752 and A549 produced additionally CXCL1/ GRO-␣, CCL2/MCP-1, and CCL5/RANTES (Table ...the cell lines released a broader spectrum of ...on cell lines when studying effects of ...
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