Human diploid fibroblasts
Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts
... Background: Human diploid fibroblasts (HDFs) undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular ...
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Efficient Quiescent Infection of Normal Human Diploid Fibroblasts with Wild-Type Herpes Simplex Virus Type 1
... normal human diploid fibroblasts at elevated ...or human fibroblast cultures (34, 37, ...of human fibroblasts, we serum starved NHDFs (75, 76) and preincubated these cultures at ...
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GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
... primary Human Diploid Fibroblasts (HDFs) as they reached the end of their pro- liferative life span ...in human tissues and thus have been proposed to con- tribute to organismal aging ...
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Gelam honey attenuated radiation induced cell death in human diploid fibroblasts by promoting cell cycle progression and inhibiting apoptosis
... In the present study we elucidated the molecular mech- anism of Gelam honey in preventing radiation-induced cell death by determining the expression of ATM, p53/73, p16 ink4a , cyclin D1, GADD45 and PAK2 genes and pro- ...
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Proteomic profiling of senescent human diploid fibroblasts treated with gamma tocotrienol
... HDFs: Human diploid fibroblasts; Hsp: Heat shock response protein; LCMS/MS: Liquid chromatography tandem mass spectrometry; LFQ: Label- free quantitative; MDH: Malate dehydrogenase; PCA: Principle ...
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Analysis of the herpes simplex virus genome during in vitro latency in human diploid fibroblasts and rat sensory neurons.
... Herpes simplex virus latency in cultured human cells following treat- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest greater than two copies of the HSV-1 genome per ha[r] ...
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Adeno-Associated Virus Type 2 Wild-Type and Vector-Mediated Genomic Integration Profiles of Human Diploid Fibroblasts Analyzed by Third-Generation PacBio DNA Sequencing
... mum edit distance between S 2 and suffixes of H using the Needleman- Wunsch algorithm (30). From all splittings of S for which the sum of these two edit distances was minimal, we selected the one with the longest S 1 . ...
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Galactose 1 phosphate uridyltransferase and galactokinase activity in cultured human diploid fibroblasts and peripheral blood leukocytes: I Analysis of transferase genotypes by the ratio of the activities of the two enzymes
... Whereas transferase activity in both fibroblasts and leukocytes was similar, galactokinase was approximately three times as active in fibroblasts as in leukocytes. All fibrobast cell strains tested had ...
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Anti-inflammatory Activity of Tocotrienols in Age-related Pathologies: A SASPected Involvement of Cellular Senescence
... of human senescent fibroblasts at vari- ous passages with a T3 rich extract was shown to reverse the senescent morphology, to decrease the ac- tivity of SA-β-gal as well as the amount of damaged DNA and ...
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Implications of oxidative stress on premature cellular senescence and development of Alzheimer's disease
... Differential response to oxidative stress in quiescent and young dividing Human Diploid Fibroblasts leading to either senescence or apoptosis: assessment of DNA damage and protein expr[r] ...
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Gamma Interferon Is a Major Suppressive Factor Produced by Activated Human Peripheral Blood Lymphocytes That Is Able To Inhibit Foamy Virus-Induced Cytopathic Effects
... activated human PBL is able to inhibit FV-induced CPE in cells of human origin but not in mouse, monkey, or hamster ...pha-1 human diploid fibroblasts (15), NIH 3T3 murine fibro- ...
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Parvovirus replication in normal and transformed human cells correlates with the nuclear translocation of the early protein NS1.
... We previously observed that H-1 infection of MRC-5 or W138 human diploid fibroblasts was nonproductive for infectious virus, but a net increase in virus could be obtained from the SV40-t[r] ...
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Anti Cutaneous Aging Effect of Red Djulis (Chenopodium formosanum) Extract on Gene Expression of Human Dermal Fibroblast
... in human skin fibroblasts reached 138% compared to the untreated control ...of human skin fibroblasts ...in human skin fibroblasts treated to extract collagen without red djulis ...
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Exosomes released from human induced pluripotent stem cells derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis
... Three human iPSC cell lines were used to generate MSCs. The first human iPSCs line (iPS-S-01) was provided by the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences, with ...
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The radiation response of human dermal fibroblasts
... two human squamous cell carcinoma lines of differing radiosensitivity has been found and this was attributed to differences in the association of the DNA to the nuclear ...
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Human endothelial cell lymphocyte interaction Endothelial cells function as accessory cells necessary for mitogen induced human T lymphocyte activation in vitro
... Mitogen-stimulated human T cell activation is absolutely dependent on the participation of a nonresponding accessory ...of human peripheral blood mononuclear cells, monocytes function as the requisite ...
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DIAMINOPURINE-RESISTANT MUTANTS OF CULTURED, DIPLOID HUMAN FIBROBLASTS
... [AS] A-selected hybrids of APRT-deficient mouse cells (clone 1D) and normal human cells prepared by DR. BRENDA KAHAN in this laboratory, regularly retain chromosome NO. This i[r] ...
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Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: The case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts
... Bourkoula, A, Constantoudis, V, Kontziampasis, D orcid.org/0000-0002-6787-8892 et al. (4 more authors) (2016) Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: The ...
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Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery
... For these reasons, the use of other types of autologous somatic stem cells is currently under investigation, such as bone marrow stem cells in a curative treatment for ischemic heart patients and cerebral infarction ...
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Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study
... (MLR), human peripheral blood mononuclear cells were isolated from one leucocyte-enriched buffycoat, obtained from Etablisse- ment Français du Sang (Paris, France), by Lymphocyte Separation Medium M (GE ...
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