human embryonic stem cell
Computational analysis of expression of human embryonic stem cell associated signatures in tumors
... Beighton G, Bello PA, Benvenisty N, Berry LS, Bevan S, Blum B, Brooking J, Chen KG, Choo ABH, Churchill GA, Corbel M, Damjanov I, Draper JS, Dvorak P, Emanuelsson K, Fleck RA, Ford A, Gertow K, Gertsenstein M, Gokhale ...
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The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines
... of human embryonic stem cell (hESC) derivatives to regenerative medicine is now becoming a ...conditions. Cell types differentiated from these designated lines may be used as a ...
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Realizing Potential: A Pragmatic Look at Human Embryonic Stem Cell Research
... n the United States, there are over 400,000 cryogenically frozen embryos (Hoffman, et al., 2003). These frozen embryos are almost exclusively produced from in vitro fertilization (IVF) and related treatments. Much debate ...
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Human Embryonic Stem Cell (hESC) and Human Embryo Research
... The American Academy of Pediatrics recognizes the ethical considerations inherent in hESC research but also recognizes the potential bene fi t to children of future discoveries, partic- ularly children for whom successful ...
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Human embryonic stem cell research, justice, and the problem of unequal biological access
... Advanced Cell Technology – have recently sought Food and Drug Administration approval to begin Phase I trials testing regenerative therapies that were developed using hES cells from discarded embryos ...hES ...
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Therapeutic potential of human embryonic stem cell transplantation in patients with cerebral palsy
... The cell lines are free of animal product and are chromosomally ...directed cell lines, non-neuronal and neuronal were obtained from a single, spare, expendable, pre implant- ation stage fertilized ovum ...
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Varicella-Zoster Virus Infects Human Embryonic Stem Cell-Derived Neurons and Neurospheres but Not Pluripotent Embryonic Stem Cells or Early Progenitors
... shown to be a cell-intrinsic inhibition of VZV replication (20, 27). Interestingly, naïve hESC have PML bodies with an atypical dis- tribution and composition (3), and it is possible that they have a role in the ...
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Human embryonic stem cell for the treatment of multiple sclerosis: A case report
... Previous studies performed for the treatment of MS using other stem cells have shown good results. Yamout et al., 2010 conducted a pilot scale study on ten patients in which they introduced the mesenchymal ...
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A molecular scheme for improved characterization of human embryonic stem cell lines
... Not all genes contribute equally to the pluripotency of hESC, of course. Recent work comparing the transcrip- tomes of multiple hESC lines has identified core sets of 105 ES-specific genes [12] and 194 genes specifically ...
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Transition toward Human Cytomegalovirus Susceptibility in Early Human Embryonic Stem Cell-Derived Neural Precursors
... FIG 3 PDGFR ␣ mediates HCMV susceptibility in early hESC-derived NPC. (A) Expression of PDGFR ␣ , as analyzed by immunostaining and flow cytometry, is shown in hESC, in neural spheres formed at the indicated times ...
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Single cell RNA seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm
... codon was cloned into the targeting vector backbone (modified from Addgene 31938) [75]. FseI and SacI linearized T-2A-EGFP-PGK-Puro cassettes were used for gene targeting experiments. All the DNA oligos used in this ...
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Recombinant extracellular matrix protein fragments support human embryonic stem cell chondrogenesis
... for cell surface receptor ...support cell attachment or chondrogenesis in the initial screen for chondroprogenitors and MSC attach- ment ...for cell attachment of induced pluripotent stem ...
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Mathematical modelling of the action potential of human embryonic stem cell derived cardiomyocytes
... from human and animal CMs represented a compulsory choice for our model, since some current properties expressed in the adult ventricular cells were unknown or not suffi- ciently characterized in ...bryonic ...
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LongSAGE profiling of nine human embryonic stem cell lines
... numerous human ESC lines, including BG01, BG02, WA01, WA07, WA09, WA13, WA14, TE06, UC01 and UC06 ...for cell line comparisons; however, incomplete annotation of the genome and inherent biases in the ...
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Human Embryonic Stem Cell Lines Model Experimental Human Cytomegalovirus Latency
... THP-1 cell lines (22) and their differentiated derivatives model the differentiation-dependent IE gene expression observed during HCMV latency, but these cells reactivate poorly or not at all, and thus are ...
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Logical Fallacies in the Literature on Human Embryonic Stem Cell Research
... embracing as "human" embryos that nature ordinarily rejects. The pro-lifers would even overrule nature itself, insisting that anything moving through the birth canal is a human being and thus ...
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Functional disruption of human leukocyte antigen II in human embryonic stem cell
... cells. Cell morphological observa- tion and RT-PCR were performed (Additional file 2: Fig- ure S1a, ...Ten cell lines were established (3 for +/+; 3 for +/–; 4 for ...mal stem cells markers in ...
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Human Embryonic Stem Cell (hESC) and Human Embryo Research
... The American Academy of Pediatrics recognizes the ethical considerations inherent in hESC research but also recognizes the potential bene fi t to children of future discoveries, partic- ularly children for whom successful ...
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Tracking the mechanical dynamics of human embryonic stem cell chromatin
... All cell types were transiently transfected with monomeric EGFP to label the entire volume of the cell, while the nuclei were stained with Hoechst 33342 to visualize the local chro- matin ...entire ...
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Effect of substrate stiffness on early human embryonic stem cell differentiation
... Substrates Cell culture substrates with varying stiffness were pre- pared from PDMS by varying the base to cross linking agent ratio from 10:1, 20:1, ...prepared cell culture substrates as determined by ...
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