Human endothelial cell culture
Human corneal endothelial cell culture and corneal transplantation
... treat endothelial dysfunctions, and in recent years DMEK have also been employed ...low endothelial cell density that most of the grafts from these age groups have, most of the grafts become ...
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Optimization of human corneal endothelial cell culture: density dependency of successful cultures in vitro
... extended culture of primary HCECs is negatively im- pacted by lower, sub-optimal plating density, and can significantly affect their proliferative ...and culture approach for the propagation of isolated ...
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Endothelial cell culture: beginnings of modern vascular biology
... Jules Hirsch Laboratory of Human Behavior & Metabolism, Rockefeller University, New York, New York, USA. In 1956, the JCI published an article by Vincent Dole on a method for titrating plasma fatty acids that ...
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Human Hepatic Sinusoidal Endothelial Cell Manual
... INTRODUCTION Human hepatic sinusoidal endothelial cells (HHSECs) are isolated from human liver obtained via the gift of organ donation from donor tissue that is not suitable for organ ...specialized ...
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Human RCAN3 gene expression and cell growth in endothelial cells
... HUVECs were seeded in 35-mm 2 dishes (six-well culture dish) two days before transfection at a density of 2x10 5 cells/ well in complete media. Transfections were performed with non-targeting, SCRAM-siRNA or a ...
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Compartmentalized Culture of Perivascular Stroma and Endothelial Cells in a Microfluidic Model of the Human Endometrium
... vascular endothelial cells exhibit morphological and biochemical changes to support embryo implantation and regulate vascular function, respec- ...of human endometrial stromal and endothelial ...
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The isolation and culture of endothelial colony forming cells from human and rat lungs
... circulating endothelial progenitor cells (EPCs) by Asahara et al 6 , postnatal vasculogenesis has been purported as an important mechanism contributing to new blood vessel formation 7,8 ...described cell ...
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Culture and identification of endothelial progenitor cells from human umbilical cord blood
... ON. Human mesenchymal stem cells support unrelated donor hematopoietic stem cells and suppress T-cell ...using human cord blood-derived endothelial ...
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Changes in Zn homeostasis during long term culture of primary endothelial cells and effects of Zn on endothelial cell senescence
... in endothelial cells at later passages, we predicted that cells would be more susceptible to cell death induced by using TPEN to deplete Zn or to Zn excess at later ...
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Human dental pulp stromal cell conditioned medium alters endothelial cell behavior
... heterogeneous cell population that can regulate the pericellular microenvironment by secret- ing ECM, growth factors and growth factor binding pro- ...optimal culture condition for ...
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The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting
... fluorescence-activated cell sorting (FACS) The expression levels of various specific osteoblast differentiation markers in the MG-63 cells were measured by real-time PCR after 120 h of ...resulting cell ...
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Inhibition of human cancer cell line growth and human umbilical vein endothelial cell angiogenesis by artemisinin derivatives in vitro
... of culture in the normal growth medium, cells were exposed to graded concentrations of ART or di- hydroartemisinin for 48 ...method, cell numbers were obtained as absorbance ...
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The addition of endothelial cell growth factor and heparin to human umbilical vein endothelial cell cultures decreases plasminogen activator inhibitor 1 expression
... in endothelial cell culture, and the isolation of PAI-1 cDNA now allows study of PAI-1 regulation at the mRNA ...of endothelial cell growth ...
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Human endothelial cell lymphocyte interaction Endothelial cells function as accessory cells necessary for mitogen induced human T lymphocyte activation in vitro
... Highly purified T cells were prepared by isolating cells rosetting with sheep erythrocytes and removing monocyte contamination by glass adherence and nylon wool column passage. When cultured at low cell density, T ...
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Urea immunoliposome inhibits human vascular endothelial cell proliferation for hemangioma treatment
... into culture flasks, and maintained in DMEM medium (GIBCO, NY) supplemented with 10% FBS (Sijiqing, Hangzhou, China) at 37°C in a humidified atmosphere of 5% CO 2 and 95% ...the endothelial cells quickly ...
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Evaluation of corneal endothelial cell therapy using an in vitro human corneal model
... organ culture model should include optimizing the hydration of the epithelial side of the cornea by using a spraying device that would enable 24-hour constant moisturizing of the ...of cell therapy over ...
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Culture and detection of primary cilia in endothelial cell models
... contrast, HMEC-1s reached confluence approxi- mately 2–3 days earlier, with a mean time of 7.2 ± 0.7 and 5.1 ± 0.3 days in low and high serum, respectively (Table 1). HUVECs showed a mixed morphology dur- ing the ...
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Human Hepatocyte Cell Culture Protocol
... the cell pellet in the bottom of the ...expected cell yield; medium type depends on subsequent ...typical cell concentration range is 1-2 x 10 6 cells/mL to allow for accurate cell ...
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Inhibition of human endothelial cell proliferation by gold compounds
... mononuclear cell infiltration and the growth of pannus are dependent on the growth of new blood ...local endothelial cell (EC) ...of human umbilical vein ...
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Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases
... ABStRAct Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in ...
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