Human hepatic stellate cells
HNE interacts directly with JNK isoforms in human hepatic stellate cells
... 4-Hydroxy-2,3-nonenal (HNE) is an aldehydic end product of lipid peroxidation which has been detected in vivo in clinical and experimental conditions of chronic liver damage. HNE has been shown to stimulate procollagen ...
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Long noncoding RNAs expressed in human hepatic stellate cells form networks with extracellular matrix proteins
... 7 cells were sheared for 5 min in 1 ml of cell lysis buffer (Covaris) using a Covaris S220 set to a peak wattage of 140, duty factor 5 % and 200 cycles per burst; immunoprecipitations were per- formed using 1 μg ...
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Growth inhibitory properties of endothelin 1 in activated human hepatic stellate cells: a cyclic adenosine monophosphate mediated pathway Inhibition of both extracellular signal regulated kinase and c Jun kinase and upregulation of endothelin B receptors
... ERK and JNK belong to the mitogen-activated protein ki- nase family and define parallel cascades that lead to the phos- phorylation of transcription factors involved in cell prolifera- tion (26, 29–31). The ERK pathway ...
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Clinical significance and gene expression study of human hepatic stellate cells in HBV related-hepatocellular carcinoma
... shared the capacity to induce genetic instability or muta- tion and subsequently fostered the carcinogenic process [3,4]. Therefore, genetic events and phenotypic variation have shed new light on the relationship between ...
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PubMedCentral-PMC5175173.pdf
... of human monocytes (THP1 cell line) and LX2 cells co-cultured in Matrigel coated plates resulted in hepatic stellate cell activation when compared to mock control as examined using ...
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Hepatic stellate cells in liver development, regeneration, and cancer
... of human liver regeneration, particularly given that the cellular and molecular pathways mediating regeneration are likely to vary somewhat depending on the nature of the initial ...of hepatic ...
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Hepatic stellate cells and parasite induced liver fibrosis
... positive cells in the fibrotic area of granulomas ...with hepatic fibro- sis and HSC activation as well as chemokines associated with HSC recruitment ...
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Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling
... LX-2 cells could be ...LX-2 cells were incubated with 50 μg/ml of CsESPs ...of hepatic fibrosis ...both human and murine tumor cell lines by in- ducing apoptosis or necrosis ...activate ...
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Hepatic stellate cells and parasite induced liver fibrosis
... new cells lines of human ...the cells to parasite antigens using techniques such as microarrays, RNAi and flow cytometry could reveal new insights into the process of parasite-induced ...
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Osteoprotegerin is More Than a Possible Serum Marker in Liver Fibrosis: A Study into Its Function in Human and Murine Liver
... in human and mouse ...of human cirrhotic and mouse fibrotic livers compared to healthy ...livers. Hepatic OPG expression localized in cirrhotic collagenous bands in and around ...liver cells ...
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Discovery of Aptamer Ligands for Hepatic Stellate Cells Using SELEX
... SELEX can be used to screen aptamers against targeting proteins [31], cells [32], specimen [33], or organs [34]. In this project, a protein-based SELEX was performed to identify IGFIIR-specific RNA aptamers, and ...
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Novel spheroid reservoir bioartificial liver improves survival of nonhuman primates in a toxin-induced model of acute liver failure
... HSCs: hepatic stellate cells; HUVECs: human umbilical vein endothelial cells; ICP: intracranial pressure; IFN: interferon; IL: interleukin; IL-1RA: IL-1 receptor antagonist; KCs: ...
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Original Article Correlation between the stromal activated hepatic stellate cells and angiogenesis in human hepatocellular carcinoma
... numerous in cirrhotic than in normal liver tissue [16, 17]. We hypothesize that activated HSCs may be derived from the peritumoral liver tis- sue. Peritumoral activated HSCs may be attr- acted to the tumor stroma in ...
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Role of MicroRNAs in Hepatic Fibrosis Progression
... of hepatic fibrosis in PDGF-C TG ...the human liver in a fibrosis progression-dependent ...LX-2 cells (human hepatic stellate cell line), also increased during the course of ...
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Human liver mesenchymal stem/progenitor cells inhibit hepatic stellate cell activation: in vitro and in vivo evaluation
... To evaluate the interactions between ADHLSCs and activated HSCs, indirect co-culture in Transwells was used. HSCs were seeded in the lower chamber of a six-well plate at a density of 1.0 × 10 5 cells/cm 2 , and ...
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PubMedCentral-PMC4992517.pdf
... Liver fibrosis is a major cause of morbidity and mortality worldwide due to chronic viral hepatitis and, more recently, from fatty liver diseases. Activation and proliferation of hepatic stellate ...
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Hepatic stellate cells: central modulators of hepatic carcinogenesis
... HCC cells, resulting in the downstream activation of angiogenic signalling ...in human HCC tissues and further assessment of it’s anti-tumourigenic activity demon- strated that this was mediated by effects ...
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Hepatic stellate cells increase in Toxoplasma gondii infection in mice
... 3.6–6 cells per 100 hepatocytes ...of stellate cell activation in the liver [19] and has been sug- gested as a more reliable marker for perisinusoidal stellate cells than desmin ...cultured ...
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The role of liver microenvironment in hepatic metastasis
... of cells that represent a fraction of a particular tissue environment ...represent hepatic tissue contain a greater representation of the liver proteins and genetic components than two-dimensional ...
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Reversal of hepatic fibrosis: pathophysiological basis of antifibrotic therapies
... obtained in a chronic CCl 4 model, even when an advanced stage of micronodular cirrhosis was obtained after 12 weeks of administration of CCl 4 . Indeed, 1 year after the cessation of administration of CCl 4 , ...
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