Human intervertebral disc
Interleukin 1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human intervertebral disc: an in situ zymographic and gene therapy study
... characterizes human intervertebral disc (IVD) ...the human IVD, and whether the IL-1 inhibitor IL-1 receptor antagonist (IL-1Ra) will inhibit ...
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The biology behind the human intervertebral disc and its endplates
... days human foetal gestation cells of the sclerotome migrate medially from pairs of paraxial somites to condense around the notochord ...the intervertebral Figure 1. Location and structure of the ...
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Expression of semaphorin 3A and its receptors in the human intervertebral disc: potential role in regulating neural ingrowth in the degenerate intervertebral disc
... phorins. Semaphorins, formerly known as collapsins, are a large family consisting of eight classes of secreted and membrane bound polypeptides that have been identified in both invertebrates and vertebrates. This family, ...
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The role of interleukin 1 in the pathogenesis of human intervertebral disc degeneration
... a disc: scores of 0 to 3 represent a histologically normal (non-degen- erate) disc; 4 to 6, histological evidence of low-level degener- ation; 7 to 9, an intermediate degree of degeneration; and 10 to 12, ...
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Pulsed Electromagnetic Field Stimulates Cellular Proliferation in Human Intervertebral Disc Cells
... volume. Disc tissues from the nucleus pulposus and inner annulus were digested for 60 minutes at 37˚C under gentle agitation in a medium composed of equal parts of Dulbecco’s Modified Eagle Medium and Ham’s F12 ...
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Nerves are more abundant than blood vessels in the degenerate human intervertebral disc
... Immunohistochemistry was used to investigate and identify neurofilament 200 (NF200, a nerve marker), protein gene product 9.5 (PGP9.5, a nerve marker) and cluster of differentiation factor 31 (CD31, a blood vessel ...
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Expression and regulation of neurotrophic and angiogenic factors during human intervertebral disc degeneration
... of matrix-degrading enzymes and also sensitise nerves, resulting in nociceptive transmission and chronic low back pain. The current study suggests IL-1β as the key regulatory cytokine involved in the upregulation of fac- ...
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Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc
... positive disc cells was noted in all regions of severely degenerate samples (grades 9 to 12), which was significant for cells in the NP and ...of disc samples of mod- erate degeneration and in the OAF of ...
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The role of interleukin 1 in the pathogenesis of human Intervertebral disc degeneration
... characterize disc degeneration, namely, decreased matrix synthesis and increased production of MMPs and ADAMTS-4 ...in human IVD ...used human cells ...on human disc cells cultured in ...
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Expression and regulation of neurotrophic and angiogenic factors during human intervertebral disc degeneration
... 53 human IVDs from 52 individuals to investigate native gene expression of neurotrophic factors and their receptors, neuropeptides and angiogenic ...in human dermal microvascular endothelial cells ...
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Review Article Immune cascades in human intervertebral disc: the pros and cons
... sion weakens the NP cells’ ability to clear the invading Fas-positive activated cytotoxic T-lymphocytes (CTLs). Taken together, these mechanisms destroy the immune balance of the microenvironment of the disc. The ...
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Nerves are more abundant than blood vessels in the degenerate human intervertebral disc
... Immunohistochemistry was used to investigate and identify neurofilament 200 (NF200, a nerve marker), protein gene product 9.5 (PGP9.5, a nerve marker) and cluster of differentiation factor 31 (CD31, a blood vessel ...
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VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration process
... in human degenerated intervertebral discs and healthy bovine caudal discs was assessed by qRT-PCR and ...across disc degeneration grades showed that membrane and soluble forms of this receptor, ...
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Asporin, a susceptibility gene in osteoarthritis, is expressed at higher levels in the more degenerate human intervertebral disc
... of human disc specimens was approved prospectively by the authors' Human Subjects Institutional Review Board at Carolinas Medical ...since disc tissue was removed as part of routine surgical ...
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Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration
... and -13 and that increased expression of IL-1Ra can reduce the activity of MMP-1, -3, -7 and -13 in tissue explants [6], we investigated the effect of TNF-α stimulation on expression of MMPs from each of these groups and ...
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Expression of the RANK/RANKL/OPG system in the human intervertebral disc: implication for the pathogenesis of intervertebral disc degeneration
... in human IVD cells demonstrated a general trend that the mRNA expression of catabolic factors stimu- lated by IL-1β was significantly suppressed by the ad- ministration of recombinant human OPG ...of ...
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Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes
... in human IVD cells revealed differences in species cell-specific expression, as well as differential expression and alterations with IVD ...between human AC and NP ...
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Biologic Treatment of Mild and Moderate Intervertebral Disc Degeneration
... degenerated intervertebral disc disease, injection of IL- 1Ra should be performed directly into the disc, because IL-1Ra cannot be delivered through systemic circulation because of the avascular ...
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Landscape of RNAs in human lumbar disc degeneration
... Accumulating evidence indicates noncoding RNAs (ncRNAs) fine-tune gene expression with mysterious machinery. We conducted a combination of mRNA, miRNA, circRNA, LncRNA microarray analyses on 10 adults’ lumbar discs. ...
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Original Article HtrA1 up-regulates expression of MMPs via Erk1/2/Rock-dependent pathways
... in intervertebral disc degeneration ...of human intervertebral disc degeneration tissues were measured by real-time quantitative PCR, and a correlation between the expression level of ...
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