Human leukocyte antigen typing
Human leukocyte antigen typing using a knowledge base coupled with a high-throughput oligonucleotide probe array analysis
... The reproducibility was assessed using repeat samples (Table 5) and repeat probes (data not shown). The array-wide probe sig- nals showed high reproducibility with the majority of repeats showing r > 0.975 for each ...
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Accurate typing of Human Leukocyte Antigen class I genes by Oxford nanopore sequencing
... The WASHU-V dataset used three genomic DNA specimens, two from the 1000 Genomes projects and one from a deidentified homozygous donor, to generate three libraries. Each multiplexed fragmented amplicons from three HLA ...
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Review Article Association between human leukocyte antigen-B and vitiligo risk: a meta-analysis
... statistically significant. The pooled ORs and corresponding 95% CIs were calculated using either the random effects model when hetero- geneity was confirmed or the fixed effects model when heterogeneity was absent. The ...
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Kourami: graph guided assembly for novel human leukocyte antigen allele discovery
... each typing locus, and may not work as well on other NGS assays, such as WES or RNA- seq ...or typing results for the other 61 alleles as some regions of these alleles were not covered by reads, causing the ...
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Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation
... Donor human leukocyte antigen (HLA)-specific antibod- ies (DSA) play an important role in solid organ trans- ...Ⅱ typing of the recipient, cytokine gene polymorphisms, and cellu- lar ...
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Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda
... in typing methods, small sample size and genetic heterogeneity in the populations studied, both in terms of ethnicity and disease manifestation, makes general conclusions on the role of specific alleles ...
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Associations between human leukocyte antigen polymorphisms and hypersensitivity to antiretroviral therapy in patients with human immunodeficiency virus: a meta analysis
... Data collection and quality assessment were carried out by two authors; any inconsistencies between these two authors were settled by group discussion until a consen- sus was reached. The data collected included the ...
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Contribution of KIR3DL1/3DS1 to ankylosing spondylitis in human leukocyte antigen B27 Caucasian populations
... HLA-B27 represents a family of at least 27 closely related alle- les (B*2701-27) that differ in their ethnic distribution. We used an SSP typing approach (PCR-SSP) capable of allelic detec- tion of all B27 alleles ...
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Prevalence of Human Leukocyte Antigen HLA B*5701 in HIV 1 Infected Individuals in Brazil
... resolution typing assays designed for this purpose must therefore achieve appropriate specificity and must be rigorously and continually exposed to quality assurance processes to ensure that HLA-B*5701 is accu- ...
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The association of human leukocyte antigen polymorphisms with disease severity and latency period in patients with silicosis
... Low-resolution typing for the HLA-A, −B, and -DR was performed by means of the PCR-sequence specific primer (PCR-SSP) method using the Micro SSP HLA class I and II generic DNA Typing Tray, Lot 008 and (One ...
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Human Leukocyte Antigen-C Class I Population -Based Ancestry Of Genetic Ethnic Diversity In Nigeria
... The cloning and sequencing of HLA class I and II genes has not only provided a clear picture of the molecular basis of allelic polymorphism, but also allowed the development of a variety of PCR-based DNA typing ...
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Human Leukocyte Antigen Genotype and Risk of HIV Disease Progression before and after Initiation of Antiretroviral Therapy
... II typing was conducted using high-resolution SSO typing for HLA-DQA, -DQB, and -DRB1 loci, using the polymorphic exon ...DRB typing was then achieved by using group-specific primers to amplify the ...
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Proline 78 Is Crucial for Human Immunodeficiency Virus Type 1 Nef To Down-Regulate Class I Human Leukocyte Antigen
... by human leukocyte antigen class I (HLA-I) molecules, encoded by the HLA-A, -B, and -C loci, on the surfaces of infected cells (13, ...of human immunodeficiency virus type 1 ...
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Biology of the human histocompatibility leukocyte antigen (HLA) system and a hypothesis regarding the generation of autoimmune diseases
... Indeed it is not yet known whether a particular polymorphism of a class II gene, i.e., a special disease allele, may be related to some autoimmune processes, but it should be emphasized [r] ...
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Changes of monocyte human leukocyte antigen DR expression as a reliable predictor of mortality in severe sepsis
... doi:10.1186/cc10457 Cite this article as: Wu et al.: Changes of monocyte human leukocyte antigen-DR expression as a reliable predictor of mortality in severe sepsis.. Submit your next ma[r] ...
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The effect of polymyxin B hemoperfusion on modulation of human leukocyte antigen DR in severe sepsis patients
... Whole blood was collected by venipuncture into hepa- rinized tubes. Polymorphonuclear cells (PMN) and mononuclear cells were extracted by one-step centrifu- gation with Polymorphprep™, which contained sodium diatrizoate ...
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A computational method for identification of vaccine targets from protein regions of conserved human leukocyte antigen binding
... The majority of traditional vaccines provide protection through neutralizing antibodies and T cells alone rarely offer protection and prevention of diseases. However, they participate in reduction, control, and clearance ...
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Relationship between human leukocyte antigen (HLA)-DQA1*0102/HLA-DQB1*0602 polymorphism and preeclampsia
... Human leukocyte antigens (HLA) play a major role in transplantation and pregnancy from the formation of gamete to the completion of the development. The alloimmune HLA-DQ sharing between the embryo ...
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Human Leukocyte Antigen Haplotypes in the Genetic Control of Immune Response to Measles-Mumps-Rubella Vaccine
... Antigen presentation and immune activation are important in immunity against measles, mumps, and rubella viruses and other pathogens. Many diseases have been associated with par- ticular HLA haplotypes [4, 26]. In ...
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Involvement of human leukocyte antigen class I molecules in human immunodeficiency virus infection of CD4-positive cells.
... Questioning the exact roles of virus-associated MHC-I molecules and cell surface-expressed MHC-I molecules during the HIV life cycle became urgent, particularly in light of the debate su[r] ...
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