human mammary epithelial cells
Cytogenetic characterization and H-ras associated transformation of immortalized human mammary epithelial cells
... Total cell protein was extracted from transduced cells by sonication in We 16 buffer (25 mM Tris HCl- pH 7.5, 125 mM NaCl, 2.5 mM EDTA, 0.05% SDS, 0.5% NP40, 0.5% DOC, 10% glycerol, 1 mM DTT, 0.5 mg/ml Pefablock, ...
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An oestrogen dependent model of breast cancer created by transformation of normal human mammary epithelial cells
... for mammary epi- thelial progenitor cells, but it is possible that expression of BMI1 promotes the survival of more differentiated cells or, conversely, forces progenitors to adopt a more ...
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Phytoestrogen (+) pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses
... MDA-MB-231 cells have not been previously ...both human breast tumour cells ...of human breast tumour cells tested, the effect was more pronounced in negative oestrogen receptor tumour ...
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EZH2 knockdown suppresses the growth and invasion of human inflammatory breast cancer cells
... SUM190 cells were cultured in Ham’s F-12 media supplemented with 10% fetal bovine serum (FBS), 1 μg/ml hydrocortisone, 5 μg/ml insulin and antibiotic- ...Primary human mammary epithelial ...
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Aptamer-labeled PLGA nanoparticles for targeting cancer cells
... cancer cells with controlled drug release for cancer ...normal human mammary epithelial cells (HMEC cells) and human glial cancer cells (GI-1 cells) by ...
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Comparative secretomic and N-glycoproteomic profiling in human MCF-7 breast cancer and HMEpC normal epithelial cell lines using a gel-based strategy
... normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line, which is of luminal epithelial origin and used as a model for estrogen ...
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Novel agents that downregulate EGFR, HER2, and HER3 in parallel
... cancer cells is presented in Figure ...MDA-MB-468 cells and the HER2 overexpressing BT474 cells between 5–10 μM (Figure ...immortalized human mammary epithelial cells ...
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Epigenome remodelling in breast cancer: insights from an early in vitro model of carcinogenesis
... Non-malignant human mammary epithelial cells (HMECs) provide an exciting in vitro model of very early breast ...of cells from the basal breast epithelial ...
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Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle
... in epithelial cells from the anatom- ically normal mammary gland (without any fibrocystic changes), including central and peripheral ducts within the lobules; myoepithelial and lymphoid cells ...
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BRCA1 mutations attenuate super enhancer function and chromatin looping in haploinsufficient human breast epithelial cells
... Our gene ontology analyses indicate that genes proximal to BRCA1-associated super-enhancers are enriched with those involved in cellular responses to various physio- logical cues including inflammation and stress. In ...
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Aldehyde dehydrogenase and estrogen receptor define a hierarchy of cellular differentiation in the normal human mammary epithelium
... ER+ cells from normal mammary epithelium and was tested for specificity and sensitivity on ER+ and ER– breast cancer cell lines (MCF7 and ...[14]. Cells were grown in phenol red-free, ...
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KISS1R Induces Human Mammary Epithelial Cell Invasiveness and Promotes Breast Cancer Cell Migration and Invasion via IQGAP1
... MCF10A cells (1 x 10 6 cells) were transfected with 5µg cDNA constructs by microporation (1700V, 10 pulse width, 3#) using the Neon TM Transfection System (Invitrogen) according to the manufacturer’s ...
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Differentiation of transformed and non-transformed human mammary luminal epithelial cells
... 5* day final cell numbers of the ErbB2 over-expressing lines, C3.6 (P<0.02) and C5.2. (P<0.02), but not of their non-transformed counterparts (HB4a, H4.1) or th[r] ...
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CCAAT/Enhancer Binding Protein-delta (C/EBP-delta) regulates cell growth, migration and differentiation
... HC11 cells cultured under the same condi- tions of confluence (Figure ...and human cells in response to growth arrest conditions ...nontransformed cells, but alterations in pSTAT3 levels and ...
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The WNT controlled transcriptional regulator LBH is required for mammary stem cell expansion and maintenance of the basal lineage
... basal epithelial characteristics by regulating transcription of genes involved in cell proliferation, adhesion and the basal cytoskeleton, including K5 (Carroll et ...luminal cells through repression of ...
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The potential role of hedgehog signaling in the luminal/basal phenotype of breast epithelia and in breast cancer invasion and metastasis
... the epithelial and mesenchymal tissue of the developing mammary gland and is essential in mammary morphogenesis ...dysplastic epithelial tissue, no detectable expression was seen distant from ...
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Basal cytokeratins and their relationship to the cellular origin and functional classification of breast cancer
... normal human breast is composed of two cell layers, an inner luminal cell population and a distinct outer cell layer, juxtaposed to the basement membrane, termed the ‘basal’ layer ...the mammary gland from ...
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Differential Expression of Human Tissue Factor in Normal Mammary Epithelial Cells and in Carcinomas
... Immunohistochemical staining of tissue factor in human normal and cancer breast tissues A section of normal breast tissue was stained positive (brown) in ductal epithelial cells with mon[r] ...
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Links between cancer stem cells and epithelial– mesenchymal transition
... tumor cells own the capacity to intravasate from the primary tumor into systemic circulation, survive in circulation, extravasate at a distant site, and proliferate in a second ...Recently, ...
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The single-cell sequencing: new developments and medical applications
... many cells, unable to analyze a small number of cells and lose cellular het- erogeneity ...vidual cells [1], distinguishing a small number of cells, and delineating cell ...
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