Human monocyte-derived dendritic cells
HIV-1 gp120 Activates the STAT3/Interleukin-6 Axis in Primary Human Monocyte-Derived Dendritic Cells
... endritic cells (DCs) play a pivotal role in linking innate and adaptive immunity by their ability to induce appropriate im- mune responses upon recognition of invading pathogens ...In human immuno- ...
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Infection of human monocyte derived dendritic cells by ANDES Hantavirus enhances pro inflammatory state, the secretion of active MMP 9 and indirectly enhances endothelial permeability
... these cells were used to infect, at a MOI of 1 for 2 h, human iDCs derived from peripheral blood monocytes (PBMC), as previously described ...
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Alteration of T cell immunity by lentiviral transduction of human monocyte derived dendritic cells
... T cells with allogeneic monocyte-derived DCs treated with TNFα and LPS to induce maturation, as illustrated in ...T cells were allowed to expand and rest for more than 7 days after DC ...T ...
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Severe Acute Respiratory Syndrome Coronavirus Fails To Activate Cytokine-Mediated Innate Immune Responses in Cultured Human Monocyte-Derived Dendritic Cells
... A549 cells, mono- cyte-derived macrophages, and DCs, since SCV-specific sub- genomic mRNAs were ...expression. Monocyte-derived human DCs were infected with SCV (MOI, 5) followed by ...
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Comparison of attenuated and virulent West Nile virus strains in human monocyte derived dendritic cells as a model of initial human infection
... mononuclear cells (PBMCs) from human buffy coat from three donors, which were tested to be negative for arboviral infections, then performed CD14+ magnetic selection to isolate ...
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Effects of Filovirus Interferon Antagonists on Responses of Human Monocyte-Derived Dendritic Cells to RNA Virus Infection
... inhibit human DC responses upon RIG-I activation, human MDDCs were transduced with empty vector, eVP35, or mVP35 ...T cells for 5 days at various DC-to-T cell ...T cells cocultured with ...
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Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods
... technologies, cells were cultured with GM-CSF and IL-4 during 1 week to allow their differentiation into MoDCs, as described in the Material and Methods ...MoDCs derived from mono- cytes isolated with MACS ...
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Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus
... To further understand the differential activation of DCs by RSV and HMPV, we investigated herein whether these viruses induce distinct profiles of microRNAs (miRNAs), a kind of small non-coding RNA molecules of 19-24 ...
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Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis
... the human dendritic cells and ...that human monocyte derived DC phagocytose ...of human monocyte-derived dendritic cells in vitro and infected ...
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Complementary Antiviral Efficacy of Hydroxyurea and Protease Inhibitors in Human Immunodeficiency Virus-Infected Dendritic Cells and Lymphocytes
... Immature monocyte-derived DC were obtained as previously described (8), with minor ...mononuclear cells (PBMC) from normal subjects and cul- tured in CM supplemented with interleukin-4 (Biosource, ...
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DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
... derived cells. Phenotypical cell markers and values of accessory activity after four and eight days of culture in MCDB-302 or medium 80/20 containing 20% human serum. Nonspecific esterase staining ...
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Evaluation of Feline Monocyte-Derived Dendritic Cells Loaded with Internally Inactivated Virus as a Vaccine against Feline Immunodeficiency Virus
... T cells capable of secreting IL-2 and other cytokines, whereas neu- tralizing antibody is envisioned as an important but difficult goal ...T cells, thanks to unique abilities as antigen-presenting ...
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Quantitative Expression and Virus Transmission Analysis of DC-SIGN on Monocyte-Derived Dendritic Cells
... MDDCs derived from our donor pool precluded us from drawing conclusions about DC-SIGN ex- pression levels and virus transmission activity on this cell ...receptor-positive cells, we screened MAbs for their ...
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Nsp1α of Porcine Reproductive and Respiratory Syndrome Virus Strain BB0907 Impairs the Function of Monocyte-Derived Dendritic Cells via the Release of Soluble CD83
... MARC-145 cells were then transfected with pCD83-luc, pRL-TK, and pCI-Nsp1 ␣ or the truncated ...promoter. Human embryonic kidney (HEK) 293T cells and MARC-145 cells were cotransfected with ...
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Dendritic Cells Transmit Human Immunodeficiency Virus Type 1 to Monocytes and Monocyte-Derived Macrophages
... Dendritic cells (DC) play a critical role in the activation of primary immune responses since they are specialized to pres- ent antigens to naive T cells in vivo ...T cells from the ...
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HIV-1 Envelope Glycan Moieties Modulate HIV-1 Transmission
... to monocyte-derived macro- phages (MDMs), (iii) bound monocyte-derived dendritic cells (MoDCs), and (iv) trans-infected primary lymphocytes via ...TZMbl cells, MDMs, ...
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Subviral Dense Bodies of Human Cytomegalovirus Stimulate Maturation and Activation of Monocyte-Derived Immature Dendritic Cells
... of dendritic cells (DC) (1, 2). Monocyte- derived, immature DC (iDC) are fully permissive to HCMV rep- lication (3), but infection of these cells is restricted to strains expressing the ...
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Generation of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison
... 10% human AB serum (Blood Transfusion Organization, Tehran, Iran), ...culture cells from peripheral blood mononuclear cells ...Recombinant human GM-CSF (Novartis- Basel, Switzerland), and IL-4 ...
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Differential Activation of Human Monocyte-Derived and Plasmacytoid Dendritic Cells by West Nile Virus Generated in Different Host Cells
... (UTMB). Human DCs were obtained as previously described ...mononuclear cells (PBMC) were obtained by Ficoll-Hypaque (GE Healthcare, Bio-sciences, Piscataway, NJ) gradient centrifugation from 100 ml of ...
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TREM2 expression in the human brain: a marker of monocyte recruitment?
... are derived from circulating ...recognise human recombinant TREM2 on Western blot, it was not able to label monocytes/macrophages, dendritic cells or osteoclasts on human tissue ...
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