Human monocyte-derived dendritic cells (MDDCs)
HIV-1 gp120 Activates the STAT3/Interleukin-6 Axis in Primary Human Monocyte-Derived Dendritic Cells
... these cells are poorly infected by HIV-1, the dynamics and functionality of dif- ferent DC subsets are strongly affected in the course of infection ...immune cells by engaging chemokine receptor entry ...B) ...
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Effects of Filovirus Interferon Antagonists on Responses of Human Monocyte-Derived Dendritic Cells to RNA Virus Infection
... modulate human MDDC responses to SeV infection. (A) Human MDDCs were mock transduced (No LV) or transduced with empty lentiviral vector (empty) or lentivirus vectors that express either Flag-tagged ...
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Alteration of T cell immunity by lentiviral transduction of human monocyte derived dendritic cells
... T cells with allogeneic monocyte-derived DCs treated with TNFα and LPS to induce maturation, as illustrated in ...T cells were allowed to expand and rest for more than 7 days after DC ...T ...
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Infection of human monocyte derived dendritic cells by ANDES Hantavirus enhances pro inflammatory state, the secretion of active MMP 9 and indirectly enhances endothelial permeability
... The presence of high levels of pro-inflammatory cyto- kines both in plasma and lungs have been reported as pathological markers associated with HCPS in humans. These excesses of pro-inflammatory cytokines are secreted in ...
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Severe Acute Respiratory Syndrome Coronavirus Fails To Activate Cytokine-Mediated Innate Immune Responses in Cultured Human Monocyte-Derived Dendritic Cells
... A549 cells, mono- cyte-derived macrophages, and DCs, since SCV-specific sub- genomic mRNAs were ...expression. Monocyte-derived human DCs were infected with SCV (MOI, 5) followed by ...
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Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods
... technologies, cells were cultured with GM-CSF and IL-4 during 1 week to allow their differentiation into MoDCs, as described in the Material and Methods ...MoDCs derived from mono- cytes isolated with MACS ...
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Comparison of attenuated and virulent West Nile virus strains in human monocyte derived dendritic cells as a model of initial human infection
... mononuclear cells (PBMCs) from human buffy coat from three donors, which were tested to be negative for arboviral infections, then performed CD14+ magnetic selection to isolate ...
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High Secretion of Interferons by Human Plasmacytoid Dendritic Cells upon Recognition of Middle East Respiratory Syndrome Coronavirus
... immune cells such as antigen-presenting cells ...B cells, macrophages, monocyte-derived/myeloid den- dritic cells (MDDCs/mDCs), and by plasmacytoid dendritic ...
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Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis
... the human dendritic cells and ...that human monocyte derived DC phagocytose ...of human monocyte-derived dendritic cells in vitro and infected ...
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Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus
... To further understand the differential activation of DCs by RSV and HMPV, we investigated herein whether these viruses induce distinct profiles of microRNAs (miRNAs), a kind of small non-coding RNA molecules of 19-24 ...
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Complementary Antiviral Efficacy of Hydroxyurea and Protease Inhibitors in Human Immunodeficiency Virus-Infected Dendritic Cells and Lymphocytes
... that dendritic cells (DC) are involved in human immunodeficiency virus (HIV) propagation to T cells and play a key role in the pathogenesis of HIV infection (5, 8, 9, 22, ...immature ...
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Subviral Dense Bodies of Human Cytomegalovirus Stimulate Maturation and Activation of Monocyte-Derived Immature Dendritic Cells
... T cells were purified from PBMC of 3 HCMV-seropositive donors and were used directly ex ...T cells from the same donors were stimulated at days 1 and 7 with irradiated PBMC, loaded with a pp65 overlapping ...
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Dendritic Cells Transmit Human Immunodeficiency Virus Type 1 to Monocytes and Monocyte-Derived Macrophages
... Transmission of HIV-1 to Mo and MDM by imDC and maDC: dose requirement, kinetics, and comparison with cell- free infection. To investigate whether DC transmit captured virus to Mo and MDM, we explored dose dependence and ...
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Differential Activation of Human Monocyte-Derived and Plasmacytoid Dendritic Cells by West Nile Virus Generated in Different Host Cells
... (UTMB). Human DCs were obtained as previously described ...mononuclear cells (PBMC) were obtained by Ficoll-Hypaque (GE Healthcare, Bio-sciences, Piscataway, NJ) gradient centrifugation from 100 ml of ...
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DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
... of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte ...the monocyte derived cells were highly viable, ...
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Evaluation of Feline Monocyte-Derived Dendritic Cells Loaded with Internally Inactivated Virus as a Vaccine against Feline Immunodeficiency Virus
... T cells capable of secreting IL-2 and other cytokines, whereas neu- tralizing antibody is envisioned as an important but difficult goal ...T cells, thanks to unique abilities as antigen-presenting ...
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Nsp1α of Porcine Reproductive and Respiratory Syndrome Virus Strain BB0907 Impairs the Function of Monocyte-Derived Dendritic Cells via the Release of Soluble CD83
... MARC-145 cells were then transfected with pCD83-luc, pRL-TK, and pCI-Nsp1 ␣ or the truncated ...promoter. Human embryonic kidney (HEK) 293T cells and MARC-145 cells were cotransfected with ...
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TREM2 expression in the human brain: a marker of monocyte recruitment?
... of human dendritic cells derived in vitro from monocytes (2) and thus TREM2 is thought to be involved in innate ...immune cells of the brain, might express ...
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HIV 2 infects resting CD4+ T cells but not monocyte derived dendritic cells
... diving cells, such as monocytes, macrophages, dendritic cells and non-activated CD4+ lymphocytes ...dividing cells such as activated CD4+ T cells, SAMHD1 is phosphorylated and does not ...
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Generation of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison
... Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in ...for human DC from peripheral blood ...autologous monocyte-conditioned medium (MCM), with heparin for ...
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