Human neutrophil
Human neutrophil gelatinase is a component of specific granules
... in human neutrophils we reacted the cells for peroxidase and then applied monospecific polyclonal antibodies to human neutrophil gelatinase to immunolabel ultrathin frozen sections using an ...
9
<p>Human Neutrophil Elastase Proteolytic Activity in Ulcerative Colitis Favors the Loss of Function of Therapeutic Monoclonal Antibodies</p>
... Human neutrophil elastase (HNE) is a serine protease that cleaves the extracellular matrix protein elastin. There is evidence that it also up-regulates a number of pro- in fl ammatory cytokines, as HNE-de fi ...
11
Primary structures of three human neutrophil defensins
... The primary structures of three human neutrophil antimicrobial peptides (HNP) were determined. The peptides, HNP-1, HNP-2, and HNP-3, which we have termed defensins, were rich in cystine, arginine, and ...
5
Identification of a human neutrophil angiotension II generating protease as cathepsin G
... A human neutrophil protease, initially termed neutral peptide-generating protease, has been shown to cleave angiotensin II directly from angiotensinogen and has been identified as leukocyte cathepsin ...
7
CAP37, a human neutrophil derived chemotactic factor with monocyte specific activity
... CAP37, an antimicrobial protein of human neutrophil granules, is a specific chemoattractant for monocytes. Purified to homogeneity by sequential chromatography over carboxymethyl Sephadex, G-75 Sephadex, ...
10
Proton secretion by the sodium/hydrogen ion antiporter in the human neutrophil
... The reducing equivalents used by the human neutrophil respiratory burst oxidase are derived from NADPH generated by the hexose monophosphate shunt. The CO2 generated by the HMP shunt is spontaneously ...
8
An adenosine triphosphate dependent calcium uptake pump in human neutrophil lysosomes
... in human neutrophil ...by neutrophil lysosomes was temperature- and pH-sensitive with optimal Ca++ pump activity at 37 degrees C and pH ...in human neutrophil lysosomes that functions ...
9
The impact of pre analytical treatment and sorting on human neutrophil function
... high neutrophil yield (all but the modified buffy coat method) and the summary of oxidative burst activation efficiency are given in Figure ...relative neutrophil counts when compared to full blood ...
10
Effects of Anti Human Neutrophil Antibodies In Vitro QUANTITATIVE STUDIES
... against human neutrophils were analyzed by means of quantitative assays to facilitate detection of antibody activity, and to probe membrane function of these ...Normal human neutrophils were purified by ...
13
Shape oscillations of human neutrophil leukocytes: characterization and relationship to cell motility
... fundamental neutrophil motor may be under tonic ...of neutrophil shape change are intrinsic properties of the ...the neutrophil with a surface serve to modulate biochemical events within the ...
7
Role of secretory events in modulating human neutrophil chemotaxis
... Inhibition of chemotaxis accompanying vigorous exocytosis can thus be related to multiple phenomena: alterations of the cytoplasmic membrane with increased cell adhesiveness and decrease[r] ...
12
Penicillin binding protein inactivation by human neutrophil myeloperoxidase
... Because PBP inactivation is microbicidal and because PBPs are located in the bacterial cytoplasmic membrane at sites potentially accessible to MPO-derived oxidants, we investigated the e[r] ...
6
Biophysics of Human Neutrophil Haptokinesis
... Neutrophils are a type of white blood cell and first responders to tissue trauma and infection. This thesis explores the role of extracellular adhesivity in dictating neutrophil phenotype with respect to cell ...
400
Human neutrophils contain and bind high molecular weight kininogen
... neutrophils was dependent upon Zn2+. Binding of 125I-HMWK to neutrophils was specific and 90% reversible. 125I-HMWK binding to neutrophils was saturable with an apparent Kd of 9-18 nM and 40,000-70,000 sites per cell. ...
9
In vitro killing of oral Capnocytophaga by granule fractions of human neutrophils is associated with cathepsin G activity
... that human neutrophil cathepsin G is an important antimicrobial system against certain oral bacteria and that cathepsin G kills bacteria by two distinct ...
Effect of needle puncture and electro acupuncture on mucociliary clearance in anesthetized quails
... Since the needling manipulation of acupuncture is a spe- cial technique, only well-trained clinicians are authorized as acupuncture practitioners or acupuncturists. The stimu- lation by different acupuncturists may vary ...
6
Host Defense Peptides in Wound Healing
... only human cathelicidin ...in human neutrophil granules (29) and var- ious cells and tissues such as T cells, monocytes, lymphocytes, natural killer (NK) cells, B cells, and mast ...in human ...
10
Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis)
... assess human neutrophil elastase, complexes together with α 1-antitrypsin were assayed in plasma by ELISA, as elastase in plasma is immediately inactivated by the formation of these covalent complexes and ...
15
Original Article Identification of CD68 neutrophil granulocytes in in vitro model of acute inflammation and inflammatory bowel disease
... against human MPO was purchased from Dako (Hamburg, Germany), a rabbit polyclonal antibody against human neutrophil elastase from Calbiochem (Germany, ...against human CD-68 (Clone KP-1, ...
10
Intestinal P glycoprotein exports endocannabinoids to prevent inflammation and maintain homeostasis
... primary human neutrophil transmigration stimulated by HxA 3 in an in vitro assay; we termed the unknown compound(s) in this preparation activity modulating epithelial neutrophil dis- course ...
14