Human nucleus pulposus cell
Identification and characterization of human nucleus pulposus cell specific serotypes of adeno associated virus for gene therapeutic approaches of intervertebral disc disorders
... positive cell detection (Table 2 and ...during cell division [34, 35, ...NP cell viability, MTT assays were per- ...the cell viabilities by 9 % and 12 %, the maximum transduction efficiencies ...
12
Expression and Role of Sphingosine 1-Phosphate Receptors in Intervertebral Disc Degeneration
... including cell migration, differentiation, inflammation and ...in human chondrocytes, S1P can inhibit the catabolic response induced by via activation of the S1P receptor[13, 14], thus the activation of the ...
7
Biologic Response of Degenerative Living Human Nucleus Pulposus Cells to Treatment with Cytokines
... living human intervertebral discs ...recombinant human bone morphogenic protein (rh- BMP-2), transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), and tumor necrosis factor-α ...living human ...
10
Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells
... in cell supplementation approaches that can maintain matrix synthesis and restore a young, nonde- generate, functional disc ...of cell delivery to pathological herniated or degenerated IVD has been ...
12
Original Article Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration
... recombinant human TGF-β1 from PeproTech (Rocky Hill, NJ, USA), collagen II antibody from ThermoFisher (Waltham, MA, USA), and calcein-AM/PI from Japan Institute Colleagues ...CCK-8 cell counting kit from ...
11
Original Article LincRNA-SLC20A1 (SLC20A1) promotes extracellular matrix degradation in nucleus pulposus cells in human intervertebral disc degeneration by targeting the miR-31-5p/MMP3 axis
... in cell growth, cell death, and inflammation remains to be further uncovered, as well as its involvement in signaling path- ...degenerative human NP cells by tar- geting the miR-31-5p/MMP ...
12
Is Osteogenic Differentiation of Human Nucleus Pulposus Cells a Possibility for Biological Spinal Fusion?
... and human NP cells maintained for 21 days with osteogenic medium (osteo) (B1-4) and its components, dexamethasone (DEX) (C1-4), β-glycerophosphate (B--G) (D1-4), L-ascorbic acid-2-phosphate (AA) (E1-4) and in ...
15
EGR1 controls divergent cellular responses of distinctive nucleus pulposus cell types
... stem cell- like properties ...NP cell types could be immortalized and clonally ...the human NP cell ...immature cell type that harbors an intrinsic capacity to respond to chondrogenic ...
13
Nucleus pulposus phenotypic markers to determine stem cell differentiation : fact or fiction?
... of human samples; such molecules include: paired box protein 1 (PAX1), forkhead box f1 (FOXF1) [10, 15] and ovostatin-2 (OVO-2) [10] as well as the proposed NP negative marker Integrin binding sialoprotein (IBSP) ...
13
Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells
... impaired cell growth in NP but not in AF ...vidual cell characteristics. This is of relevance as cell-based therapeutic approaches often utilise herniated IVD tissue as starting tissue material for ...
14
Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model
... in cell numbers and altered cell function in the nucleus pulposus (NP), resulting in an imbalance between matrix synthesis and ...stem cell therapy, ...
19
Original Article Resveratrol inhibits TNF-α-induced matrix degradation via the p38/MAPK and Akt pathways in human nucleus pulposus cells
... were blocked with 5% nonfat milk in TBST and incubated overnight at 4°C in 5% nonfat milk in TBST with the anti-Collagen II antibody (1:1000, Abcam); anti-Aggrecan antibody (1:1000), anti- GAPDH (1:1000), anti-p65 ...
9
Synergistic role of c Myc and ERK1/2 in the mitogenic response to TGFβ 1 in cultured rat nucleus pulposus cells
... determine cell proliferation, nucleus pulposus cells were plated in 96-well plates at a density of 3,000 ...recombinant human TGF1 in final concentra- tions of 0 (control), 5, or 20 ...
12
Original Article Expression of soluble Fas and soluble FasL in human nucleus pulposus cells
... dence has indicated that molecular mecha- nisms are indispensable parts [6, 7]. In fact, the local expression of Fas and Fas ligand (FasL) in NP has been noted as the underlying mecha- nism of immune privilege of the ...
7
miR-573 regulates cell proliferation and apoptosis by targeting Bax in nucleus pulposus cells
... death [29, 30]. Bax is a member of the Bcl-2 family. It has been well documented that increased Bcl-2 expression and decreased Bax expression reduced mitochondrial mem- brane permeability and suppressed the release of ...
10
Acidification changes affect the inflammasome in human nucleus pulposus cells
... (Science Cell, Carlsbad, CA) cells were obtained from the spinal column (isolated from the nu- cleus pulposus of human intervertebral discs) of a male donor that did not present spine degenerative ...
11
Novel immortal human cell lines reveal subpopulations in the nucleus pulposus
... 70-μm cell strainer and collected by centrifugation (280 × g, 5 minutes, Eppen- dorf centrifuge 5810R) and cultured in maintenance medium (Mmed) (DMEM-F-12/GlutaMAX, 10% foetal calf serum (DE14-801 F; ...
16
Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype
... both human and rat IVD development ...NC cell conditioned medium (NCCM) by mass spectroscopy [16] and is highly differ- entially expressed by NC, NP and AF cells ...
9
Original Article RASSF7 expression and its regulatory roles on apoptosis in human intervertebral disc degeneration
... for cell growth and ...in human degenerative nucleus pulposus (NP) cells and non-degenerative NP cells and the link between RASSF7-JNK with NP cells ...in human IDD and RASSF7 might be ...
7
Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
... 2 positive (GD2+) cell fraction from human and mouse IVD tissues. These cells were described to derive from the Tie2+ and GD2– precursor cells and are capable of differentiating into multiple mesenchymal ...
12