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Human osteoblast

Human osteoblast growth and maturation in response to metformin and the thienopyridone, A769662

Human osteoblast growth and maturation in response to metformin and the thienopyridone, A769662

... The human osteoblast cell lines, MG63 and Saos-2 and commercial primary human osteoblasts (hOBs, Promocell, Heidelberg, Germany) were ...of osteoblast growth and maturation were determined ...

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IL-1β Inhibits Human Osteoblast Migration

IL-1β Inhibits Human Osteoblast Migration

... Bone has a high capacity for self-renewal and repair. Prolonged local secretion of interleukin 1β (IL-1β), however, is known to be associated with severe bone loss and delayed fracture healing. Since induction of bone ...

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Local origins impart conserved bone type-related differences in human osteoblast behaviour

Local origins impart conserved bone type-related differences in human osteoblast behaviour

... primary human osteoblasts have not regarded the possibility that their site of origin from within a bone may influence ...the human humerus from OA and OP patients undergoing shoulder replacement surgery, ...

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The effects of zoledronate on the survival and function of human osteoblast like cells

The effects of zoledronate on the survival and function of human osteoblast like cells

... After a 48-h ZOL treatment, the osteoblast proliferation rate was dose-dependently downregulated (Fig. 1a). There were significant differences between the ZOL- treated and vehicle-treated osteoblasts, with p < ...

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Local origins impart conserved bone type-related differences in human osteoblast behaviour

Local origins impart conserved bone type-related differences in human osteoblast behaviour

... primary human osteoblasts have not regarded the possibility that their site of origin from within a bone may influence ...the human humerus from OA and OP patients undergoing shoulder replacement surgery, ...

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Silencing Dkk1 expression rescues dexamethasone induced suppression of primary human osteoblast differentiation

Silencing Dkk1 expression rescues dexamethasone induced suppression of primary human osteoblast differentiation

... primary human osteoblasts treated with dexamethasone was measured by quantita- tive Real Time PCR using human Dkk-1 QuantiTect assay (Qiagen, Crawley, ...

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Influence of the Biofield Energy Treated Vitamin D3 on Human Osteoblast-Like Cells

Influence of the Biofield Energy Treated Vitamin D3 on Human Osteoblast-Like Cells

... item, a Complementary and Alternative Medicine (CAM) on bone health using MG-63 cell line for major biomarkers. Within the burgeoning ground of CAM therapies, Biofield Energy Treatment or energy medicine, is emerging ...

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IN VIVO AND IN VITRO STUDIES ON JOINT REGENERATIVE POTENTIAL OF FISH SKIN DERIVED COLLAGEN PEPTIDES

IN VIVO AND IN VITRO STUDIES ON JOINT REGENERATIVE POTENTIAL OF FISH SKIN DERIVED COLLAGEN PEPTIDES

... Marine fish skin derived bioactive peptides, based on their structural properties with specific sequence of amino acids represent an important group of molecules having various biological functions. This study was ...

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Phytase mediated enzymatic mineralization of chitosan enriched hydrogels

Phytase mediated enzymatic mineralization of chitosan enriched hydrogels

... increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme ...

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Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study

Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study

... and osteoblast adhesion as compared with traditional micro-sized HA [12,13], stimulation of human osteoblast-like cell proliferation resulting in bone formation [14], and the stimulation of PDL cell ...

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Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

... Notes: Human osteoblast-like MG63 cells were seeded on chitosan nanofiber scaffolds for 1, 3, and 5 ...of human osteoblasts was determined by crystal violet staining ...

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Comparative Response of CRL 11372 Cells to Surface Roughness and Crystalline Structure of the Surfaces Developed by Sandblasting, Etching, and TiO2 Coating on Commercially Pure Ti Discs

Comparative Response of CRL 11372 Cells to Surface Roughness and Crystalline Structure of the Surfaces Developed by Sandblasting, Etching, and TiO2 Coating on Commercially Pure Ti Discs

... that human osteoblast-like cells were supposed to be more sen- sitive to the organization and morphology of roughness rather than to amplitude ...that osteoblast-like cells lacked adaptation to ...

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Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

... overnight with Dulbecco’s Modified Eagle Minimum Essential Medium (Sigma). The samples were then seeded with human osteoblast-like MG-63 cells (European Collection of Cell Cultures, Salisbury, UK). Each ...

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Selective inhibition of MG-63 osteosarcoma cell&nbsp;proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

Selective inhibition of MG-63 osteosarcoma cell&nbsp;proliferation induced by curcumin-loaded self-assembled arginine-rich-RGD nanospheres

... efficiently and induce significantly higher cellular uptake than in human osteoblast cells. With an RGD-functionalized head group, the curcumin-loaded micelles might selectively attach to the receptors of ...

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DETECTION OF BETA ACTIN AND OSTEOCALCIN GENES EXPRESSION IN DIFFERENTIATED OSTEOBLASTS BY RT PCR

DETECTION OF BETA ACTIN AND OSTEOCALCIN GENES EXPRESSION IN DIFFERENTIATED OSTEOBLASTS BY RT PCR

... into osteoblast was induced by osteogenic medium consisting of high glucose- DMEM supplemented with Ascorbic acid, dexamethasone and beta glycerophosphate disodium salt hydrate after 21 ...

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The PPAR-γ antagonist T007 inhibits RANKL-induced osteoclastogenesis and counteracts OVX-induced bone loss in mice

The PPAR-γ antagonist T007 inhibits RANKL-induced osteoclastogenesis and counteracts OVX-induced bone loss in mice

... the osteoblast-specific ...the osteoblast-specific genes Runx2, OCN, ALP, and COL1a in BMSCs with T007-treated maintained in osteogenic medium for 7 ...the osteoblast-related genes Runx2, OCN, ALP, ...

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The shift in the balance between osteoblastogenesis and adipogenesis of mesenchymal stem cells mediated by glucocorticoid receptor

The shift in the balance between osteoblastogenesis and adipogenesis of mesenchymal stem cells mediated by glucocorticoid receptor

... induced by mechanical forces applied through β1 integrins [162, 163]. The Rac GTPase-activating protein (GAP) FilGAP regulates cellular activity such as actin assembly [164] and targets sites of FLNA force transfer ...

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Overexpression of carboxypeptidase X M14 family member 2 predicts an unfavorable prognosis and promotes proliferation and migration of osteosarcoma

Overexpression of carboxypeptidase X M14 family member 2 predicts an unfavorable prognosis and promotes proliferation and migration of osteosarcoma

... Currently, the specific impact of CPXM2 in osteosar- coma remains unclear. As EMT is a critical process that mediates tumor progression and metastasis, we postulate that CPXM2 may catalyze key molecules that regulate EMT ...

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Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma

<p>Circular RNA circTUBGCP3 Is Up-Regulated and Promotes Cell Proliferation, Migration and Survivability via Sponge mir-30b in Osteosarcoma</p>

... Materials and Methods: In this study, circTUBGCP3 was chosen from the existing reported circRNA microarray data obtained from OS cell lines and normal bone cells. Subsequently, qRT-PCR was performed to evaluate the ...

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The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells

The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells

... exposed human periosteal-derived stem cells on day ...that human periosteal-derived stem cells were differentiated into osteoblasts and involved in the matrix ...

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