Human Peripheral Blood
CYTOTOXIC EFFECT OF JUGLONE ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES
... Human peripheral blood lymphocytes were cultures in varying concentrations of juglone for different time periods.DNA Fragmentation is one of the hallmarks of apoptosis. To study whether the juglone ...
9
CD40 expression by human peripheral blood eosinophils
... in human peripheral blood eosino- ...whether human peripheral blood eosinophils express CD40, we first examined mRNA expression using RT- ...with human CD40 ...man ...
7
Differential processing of proenkephalin A by human peripheral blood monocytes and T lymphocytes
... Human peripheral blood mononuclear cells are analyzed for preproenkephalin gene expression and peptide processing. Met-enkephalin immunoreactivity as detected with a specific antiserum is found in ...
9
Surface immunoglobulin on activated human peripheral blood thymus derived cells
... Human peripheral blood lymphocytes grown in vitro were stimulated with nonspecific mitogens and in mixed lymphocyte culture. The presence of IgM and thymus (T) surface markers on large and small ...
11
Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts
... in human peripheral blood monocytes ...that peripheral monocytes differentiate into osteoclasts when seeded on RANKL/osteoclast differentiation factor-producing RA syno- vial fibroblasts ...
14
Effects of PM10 in human peripheral blood monocytes and J774 macrophages
... incubated at 37°C until required for the assay. One milli- litre of cell suspension was transferred to an Eppendorf tube, centrifuged at 145 g for 2 minutes, the medium removed, the cell pellet resuspended in 1 ml PBS ...
12
Nonpermissivity of human peripheral blood lymphocytes to adenovirus type 2 infection.
... In Epstein-Barr virusnegative Jijoye cells, adenovirus infection caused an increase in the production of Epstein-Barr virus antigen, showing an interesting interaction between two DNA tu[r] ...
5
Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... diluted blood samples were gently layered and isolated by density centrifugation on Ficoll-Hypaque® gradients (Sigma- Aldrich, UK) using two different centrifugation programs at room temperature (20 minutes at 700 ...
6
Replication of human cytomegalovirus in human peripheral blood T cells.
... This prompted us to investigate the comparative replication of HCMV in different human leukocyte subpopulations with special regard to T lymphocytes stimulated in the allogeneic mixed ly[r] ...
8
Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection.
... Difference in expression of HCMV IE and E antigensa in in vitro-infected PBMC cultured in FCS or AB serum and content of infectious virus" HCMV antigen or plaques Phenotype of cells expr[r] ...
10
Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).
... two human cell types: i) human peripheral blood mononuclear cells (PBMC), and ii) human hepatoma cell line (HepG2), respectively, to evaluate its direct effects and after liver enzymes ...
12
Chemical and immunological characterisation of glycophospholipid and phospho-oligosaccharide from mycobacteria
... Human peripheral blood mononuclear cells (PBMCs) were separated from the blood of healthy male ...venous blood was drawn into a container with approximately 5U/ml sterile, preservative ...
246
Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment
... the human keratinocyte cell line and human peripheral blood mononuclear cells revealed that their cytotoxicity may be limited by modulating the employed concentrations of ...
10
C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes
... plexes with anticancer drugs Cis and Dox on intracellular ROS generation of nonsen- sitized human peripheral blood phagocytes in buffy coat. The treatment exposure time was 30 min. It suggests the ...
22
Differential expression of Ia molecules by human monocytes
... adherent, human peripheral blood ...all human peripheral blood monocytes display easily detectable HLA-DR molecules, only 50% display easily detectable HLA-DC ...of ...
9
Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity
... An immune response is triggered upon AMI that, although essential for proper tissue remodeling and stabilization, carries unwanted inflammatory-mediated damage [13] and, in the case of cell therapy approaches, might be ...
7
Interaction of human leukocytes and Entamoeba histolytica Killing of virulent amebae by the activated macrophage
... competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (PBMC), monocytes (MC), and MC-derived ...
10
Post - prandial rise of microvesicles in peripheral blood of healthy human donors
... for blood sample processing were optimal for less than 24 samples per day, so subjects were organized into 2 groups, the first group consisting of 23 subjects and the second one consisting of 10 sub- ...staining, ...
11
Proinsulin, insulin, and C peptide concentrations in human portal and peripheral blood
... and peripheral venous blood in six nondiabetic, nonobese ...In peripheral venous blood, maximal levels of the three peptides were observed later (2-5 min), and the increase in insulin relative ...
7
Human leukocytic pyrogen induces release of specific granule contents from human neutrophils
... Hypaque and dextran sedimentation were exposed to purified human LP. The specific granule-associated proteins, lysozyme and lactoferrin were selectively released, whereas primary granule (beta-glucuronidase) and ...
8