Human peripheral-blood mononuclear cell
The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody dependent enhancement of infection with dengue virus serotype 3
... Dengue virus (DENV) is a mosquito-borne virus that causes serious widespread public health issues in tropic and sub-tropical areas of the world. There are more than 100 countries where DENV is endemic, and the hardest ...
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Potent Anti-R5 Human Immunodeficiency Virus Type 1 Effects of a CCR5 Antagonist, AK602/ONO4128/GW873140, in a Novel Human Peripheral Blood Mononuclear Cell Nonobese Diabetic-SCID, Interleukin-2 Receptor γ-Chain-Knocked-Out AIDS Mouse Model
... 1.84 (Fig. 5B) was seen in PBMC recovered from an AK602- treated mouse, and the size of this CD4 ⫹ cell population was comparable to that seen in a ddI-treated mouse (53.2% [3.8% ⫹ 49.4%]) and that in an ...
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Interaction of Borrelia burgdorferi with Peripheral Blood Fibrocytes, Antigen-Presenting Cells with the Potential for Connective Tissue Targeting
... When incubated with a human peripheral blood mononuclear cell preparation, we find many profiles of phagocytic cells where the Bor- reliae are internalized by coiling phagocytosis, as il[r] ...
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<p>Selective purging of human multiple myeloma cells from peripheral blood mononuclear cells: a preliminary study</p>
... The human myeloma cell line RPMI-8226 (Seoul, Korea) was treated with bortezomib (Selleck Chemicals, Houston, TX, USA) or lenalidomide (Sigma Aldrich, ...the human peripheral blood ...
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Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... diluted blood samples were gently layered and isolated by density centrifugation on Ficoll-Hypaque® gradients (Sigma- Aldrich, UK) using two different centrifugation programs at room temperature (20 minutes at 700 ...
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Detection of human T cell lymphotropic virus type I proviral DNA and its gene expression in synovial cells in chronic inflammatory arthropathy
... the peripheral blood mononuclear cells (PBMCs) and synovial fluid cells (SFCs), but also in the T lymphocyte-depleted cultured synovial cells ...
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Functional Measurement of Hepatitis C Virus Core Specific CD8+ T Cell Responses in the Livers or Peripheral Blood of Patients by Using Autologous Peripheral Blood Mononuclear Cells as Targets or Stimulators
... the peripheral blood and because the targeted epitopes vary depending on the human leukocyte antigen (HLA) types of the ...autologous peripheral blood mononuclear cells (PBMCs) ...
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Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture
... Background: Third (infective)-stage Gnathostoma spinigerum larvae (L3) mainly cause human gnathostomiasis. G. spinigerum L3 migrate throughout the subcutaneous tissues, vital organs, and central nervous system and ...
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Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).
... The evaluation of the genotoxic potential of chemical and pharmaceutical compounds is of fundamental importance, considering the relationship between mutation and human diseases including cancer (Tennant, 2014). ...
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Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment
... the human keratinocyte cell line and human peripheral blood mononuclear cells revealed that their cytotoxicity may be limited by modulating the employed concentrations of ...
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Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats
... Methods: Mononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned ...
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Monocytotropic human immunodeficiency virus type 1 (HIV-1) variants detectable in all stages of HIV-1 infection lack T-cell line tropism and syncytium-inducing ability in primary T-cell culture.
... In studies on human immunodeficiency virus type 1 HIV-1 isolates obtained from peripheral blood mononuclear cell PBMC samples from infected individuals by coculture with phytohemagglutin[r] ...
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Interaction of human leukocytes and Entamoeba histolytica Killing of virulent amebae by the activated macrophage
... competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (PBMC), monocytes (MC), and ...
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Relapses of primary cutaneous anaplastic large-cell lymphoma in a female immunocompetent patient with persistent chlamydophila pneumoniae and human herpesvirus 8 infection
... Findings: During the 4 years following the surgical excision of the first PCALCL, the patient developed five further skin lesions located at different anatomical sites, all histologically proven as PCALCLs. The patient ...
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Some Aspects of the Normal Role of Neuromodulators in the Immune System
... the human immu- nodeficiency virus type 1 via NF-kappaB in peripheral blood mononuclear cells (PBMC) ...in human PBMC ...of human peripheral blood mononu- clear ...
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Chemical and immunological characterisation of glycophospholipid and phospho-oligosaccharide from mycobacteria
... Human peripheral blood mononuclear cells (PBMCs) were separated from the blood of healthy male ...venous blood was drawn into a container with approximately 5U/ml sterile, ...
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Distribution and Evolution of T-Cell Receptor Vβ Repertoire on Peripheral Blood Lymphocytes of Newborn Infants of Human Immunodeficiency Virus (HIV)-Infected Mothers: Differential Display on CD4 and CD8 T Cells and Effect of HIV Infection
... Neonatal human peripheral blood mononuclear cells from 12 human immunodeficiency virus (HIV)-infected and 84 uninfected children were assessed for their distribution of T-cell ...
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Recovery of Pathogenic Measles Virus from Cloned cDNA
... as human peripheral blood mononuclear cells (PBMCs) and a monkey B95a cell line, whereas MeV strains passaged in non- lymphoid cells, such as Vero cells, are remarkably attenuated in ...
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Optimizing the method for generation of integration-free induced pluripotent stem cells from human peripheral blood
... red blood cells, while with the combination of HES and Ficoll most of the red blood cells could be Table 1 Human iPSCs generated from PB with the optimized ...
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Immunodominant HIV-Specific CD8+ T-Cell Responses Are Common to Blood and Gastrointestinal Mucosa, and Gag-Specific Responses Dominate in Rectal Mucosa of HIV Controllers
... Even though plasma viremia in subjects on long-term HAART may be comparable to that in elite controllers (28), subjects on HAART have much lower HIV-specific T-cell re- sponses (as defined by magnitude and ...
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