human retinal pigment epithelial
Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture
... Low-level, steady electric fields of 6-10 voltskm stimulated directional orientation and translocation of cultured human retinal pigment epithelial cells.. The orientative movements galv[r] ...
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Altered aldose reductase gene regulation in cultured human retinal pigment epithelial cells
... several human retinal pigment epithelial cell lines exposed to hyperglycemic and hyperosmolar conditions in ...several retinal pigment epithelial cell ...
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Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus
... Short reads were filtered for adapters and reads of low quality using Cutadapt (v.1.8) [8] with error rate of 0.2 and minimum length of 18 bp, aligned against GEN- CODE human genome reference assembly GRCh38. p3 ...
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Original Article Plasticity of adult human retinal pigment epithelial cells
... Abstract: Retinal pigment epithelium (RPE) cell damage underlies many degenerative, dystrophic, and proliferative diseases of the human ...adult human RPE cells can be used for in vitro ...
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Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid
... Here, we have shown that AF was able to promote ret- inal progenitor gene expression levels in 30% AF control cultures, while immunocytochemical analysis of 30% AF control cultures indicated a decrease in the number of ...
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Resveratrol inhibits transforming growth factor-β2-induced epithelial-to-mesenchymal transition in human retinal pigment epithelial cells by suppressing the Smad pathway
... following retinal detachment ...(TGF)-β2-induced epithelial-to-mesenchymal transition (EMT) plays an important role in the development of PVR, and EMT inhibition decreases collagen gel contraction and ...
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Synergistic effects of gamma interferon on inflammatory mediators that induce interleukin-6 gene expression and secretion by human retinal pigment epithelial cells.
... INFLAMMATORY MEDIATORS AND IL-6 SECRETION BY RPE CELLS 577 human retinal pigment epithelium, p. Marmor (ed.), The retinal pigment epithelium[r] ...
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Ion channels of cultured human retinal pigment epithelial cells
... diseased human eye, and if fundamental differences exist between amphibian and mammalian transport mechanisms, this ultimate goal is best achieved with the study of mammalian ...
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Reactive oxygen intermediates increase vascular endothelial growth factor expression in vitro and in vivo
... man retinal pigment epithelial cells exposed to superoxide or hydrogen peroxide rapidly increased VEGF mRNA lev- ...of human retinal pigment epithelial cells in vitro and ...
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Wogonin modulates hydroperoxide-induced apoptosis via PI3K/Akt pathway in retinal pigment epithelium cells
... Akt vitality. Akt activation enhances RPE cell survival and thus may protect RPE cells from oxidant-induced cell death under normal circumstances and in abnormal states such as AMD [20]. Byeon SH et al. showed that ...
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HIV 1 Tat protein alter the tight junction integrity and function of retinal pigment epithelium: an in vitro study
... Methods: A human RPE cell line (D407) cultured on microporous filter-supports was used. After treating with HIV-1 Tat protein, transepithelial electrical resistance (TER) of confluent RPE cells was measured by ...
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Optimization of an in vitro bilayer model for studying the functional interplay between human primary retinal pigment epithelial and choroidal endothelial cells isolated from donor eyes
... primary retinal pigment epithelial cells (hRPE) and human primary choroidal endothelial cells (hCEC) from donor eyes to construct a bilayer of hCEC/hRPE on transwell ...based human ...
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Original Article Effect of bradykinin on cultured retinal pigment epithelial cells
... The human retinal pigment epithelia cell line ARPE-19 was donated by Professor Guotong Xu from the Tongji Eye Institute. ARPE-19 cells were cultured in RPE medium (Dulbecco’s mod- ified Eagle’s ...
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Osmolarity and spectrophotometric property of brilliant blue green define the degree of toxicity on retinal pigment epithelial cells exposed to surgical endoilluminator
... Conclusion: Our findings suggest that BBG at 0.25 mg/mL during vitreoretinal surgery is safe and not toxic to RPE cells up to 5 minutes under focal high illumination (10 mm) and up to 15 minutes under medium diffuse ...
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OX40 ligand expression abrogates the immunosuppressive function of retinal pigment epithelium
... cytokine-stimulated ARPE cells were isolated and analyzed for OX40L expression by reverse transcription-polymerase chain reaction. Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human ...
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Retinal and choroidal changes in steroid-associated central serous chorioretinopathy
... The clinical examination included assessment of the best corrected visual acuity (BCVA) in Snellen, spheri- cal equivalent of refractive status of the eye, slit lamp biomicroscopy with a contact lens or non-contact lens, ...
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Progress and perspectives on the role of RPE cell inflammatory responses in the development of age-related macular degeneration
... in retinal microglia cells (macrophages and DCs) and mediates migration and adhesion of these cells in response to its ...of retinal degeneration and CNV (Combadiere et al ...AMD-like retinal lesions ...
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Flammer Syndrome, A Potential Risk Factor for Central Serous Chorioretinopathy?
... of CSCR. Choroidal blood-flow changes can be induced through the production of free radicals, inflammatory cells, and nitric oxide. Long-standing choroidal changes and vein congestion together with the extensive ...
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Functional Genetic Analysis of Rhesus Cytomegalovirus: Rh01 Is an Epithelial Cell Tropism Factor
... If it is not a constituent of virions, how could pRh01 influ- ence the infectivity of virus particles produced in RRPE cells? In polarized epithelial cells, there are distinct sorting pathways for cargo destined ...
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Formononetin, an active compound of <em>Astragalus membranaceus</em> (Fisch) Bunge, inhibits hypoxia-induced retinal neovascularization via the HIF-1α/VEGF signaling pathway
... cular areas were assessed through visualization of the retinal vasculature using infusion of ADPase staining, examined under fluorescent microscopy. In each whole mount, the total areas of preretinal neovascular ...
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