Human β-defensin-3
Human papillomavirus oncogenic E6 protein regulates human -defensin 3 (hBD3) expression via the tumor suppressor protein p53
... Human β -defensin-3 (hBD3) is an epithelial cell-derived innate immune regulatory molecule overexpressed in oral dysplastic lesions and fosters a tumor- promoting ...high-risk human ...
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Respiratory epithelial cells require Toll-like receptor 4 for induction of Human β-defensin 2 by Lipopolysaccharide
... seeded in 8 well chamber slides (Nunc). Slides were fixed in methanol (AnalaR) for 5 minutes and labelled with goat anti-HBD-2 antibody (a gift from Dr Paul McCray, University of Iowa, Iowa City, IA, USA) 1:100 dilution) ...
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Effects of Stress on Mouse β Defensin 3 Expression in the Upper Digestive Mucosa
... the mBD-3 mRNA level was higher in the gingiva than in the stomach. These differences may be related to differenc- es in mucosal surface structure of the gingiva, esophagus, and stomach. Minimal mBD-3 mRNA ...
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Human β defensin 2 plays a regulatory role in innate antiviral immunity and is capable of potentiating the induction of antigen specific immunity
... its 3′ terminus was amplified by polymerase chain reaction (PCR) using the forward primer (5′-GAG CTC AAG TAT TAT TCT ATC ATT CCT-3′, where the underlined letters represent the SacI restriction site) with ...
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Antimicrobial peptide β-defensin-1 expression is upregulated in Alzheimer’s brain
... Mammalian β-defensins are an evolutionarily conserved group of cationic, non-glycosylated antimicrobial and immunomodulatory peptides that are expressed in all mammals, including humans ...pressed human ...
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Isoleucine, an Essential Amino Acid, Induces the Expression of Human β Defensin 2 through the Activation of the G Protein Coupled Receptor ERK Pathway in the Intestinal Epithelia
... and β defensins, which belong to the defensin family, have been shown to play pivotal roles through their powerful anti-microbial activity ...Conversely, β-defensin (HBD) is produced by the ...
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Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter
... While accurate quantification of the mature hBD2 expression levels has not been performed in this study, the intensity of bands observed by SDS-PAGE suggests that high-levels of hBD2 production might be achieved during ...
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Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection
... uginosa rather than against E. coli, along with its stronger antimicrobial activity relative to that of hBD-2, under- scores the deep involvement of hBD-4 in the innate immunity of the lower respiratory tract. The ...
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Legionella pneumophila induces human beta Defensin-3 in pulmonary cells
... that human β- defensins (hBDs), a family of endogenous, cationic anti- microbial and immunomodulatory peptides secreted at epithelial mucosal and macrophages are critical compo- nents of host defense ...The ...
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THE RELATIONSHIP BETWEEN INTESTINAL PROTECTIVE MARKERS AND SERUM ENDOTHELIN 1 CONCENTRATIONS IN LOW BIRTH WEIGHT INFANTS WITH HYPOXIC ISCHEMIC ENCEPHALOPATHY
... Azerbaijan Medical University, K. Farajova Scientific Research Institute of Pediatry, Baku, Azerbaijan. ABSTRACT: Perinatal hypoxia results in poor circulation in internal organs, causing damage to the intestinal mucosa. ...
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Fecal human β-defensin-2 (hBD-2) levels and gut microbiota patterns in preterm neonates with different feeding patterns
... Measurement of hBD-2. Fecal hBD-2 was mea- sured using a specific enzyme-linked immune assay (ELISA) according to the manufacturer’s instruc- tions (β–defensin 2; Immunodiagnostic AG, Ben- sheim, Germany; ...
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Clinical application of human β defensin and CD14 gene polymorphism in evaluating the status of chronic inflammation
... others. Human b- defensins (hBDs) are a group of low molecular weight (3 to 5 kDa) cationic antimicrobial peptides with cysteine- rich ...[21]. Human ß defensins, namely hBD-1 and hBD-2, have been ...
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Synergistic effect of interleukin 1 alpha on nontypeable Haemophilus influenzae induced up regulation of human beta defensin 2 in middle ear epithelial cells
... of β-defensin 2 promoter ...of β -defensin 2 (DEFB4) (AF_071216) was isolated by PCR amplification with the following primers: 5'-GAG GTA CCT CCA TCC TTT ACT GTG ATG ATG CC-3' (forward ...
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The potential for immunoglobulins and host defense peptides (HDPs) to reduce the use of antibiotics in animal production
... The adjunctive use of HDPs in antibiotic therapy has been examined against experimental tuberculosis, systemic E. coli infection and cerebral malaria. M. tuberculosis (TB) infected mice were subcutaneously treated 15 ...
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Keratinocyte Expression of Human β Defensin 2 following Bacterial Infection: Role in Cutaneous Host Defense
...  defensin 2 (hD-2) is a cationic antimicrobial peptide (CAP) found in the skin and is thought to play impor- tant roles in cutaneous host immune defense and numerous disease ...
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Human beta defensin 1: an antimicrobial peptide of urogenital tissues
... of human b ...from human blood filtrate, and the corresponding cDNA fragment was cloned from kidney and vaginal cDNA libraries ...of human mRNA (Clontech) with our full length HBD-1 cDNA ...
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Host Defense Peptides in Wound Healing
... induces human β-defensin 2 (88,89). Further- more, human Toll-like receptor 2 medi- ates induction of the antimicrobial pep- tide hBD-2 in response to bacterial lipoprotein ...
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Ileal antimicrobial peptide expression is dysregulated in old age
... as β -defensin 1, angiogenin 4 and Relmb, which are made by several epithelial cell types, was significantly upregulated in aged animals suggesting an ongoing response to epithelial ...
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A study on β-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression
... The study was conducted between 2010 and 2013 and included 82 pediatric patients from the Department of Pediatric Dentistry, Institute of Dentistry, Jagiellonian University, Medical College in Krakow, Lesser Poland ...
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Human beta-defensin 3 contributes to the carcinogenesis of cervical cancer via activation of NF-κB signaling
... induce the release of hBD-3 and induce the production of inflammatory cytokines [5, 23, 28, 29]. To be noted, it has also been found that expression of hBD3 in the intestine was suppressed by Shigella flexneri, a ...
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