Hybridisation and detection
In situ hybridisation for the detection of viral nucleic acids
... situ hybridisation as a means of analysis of the effects on viral replication of experimental perturbation of the host's Immune ...situ hybridisation for detection of viral nucleic acids, together ...
257
Impedimetric measurement of DNA-DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)
... the detection of methicillin resistance in clinical ...for detection of nucleic acid hybridisation from clinical ...gene detection and measurement of clinically important DNA ...
15
Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... blot hybridisation assay was developed within this ORF1- ORF2 junction by designing 25 genotype specific probes to allow rapid detection and typing of an ...
8
Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
... situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using ...
6
Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
... Avoiding this by use of lysates provides a great benefit compared to RT-PCR and array based methods, which al- ways require a separate RNA purification step. Use of high concentrations of either cell lysate or total RNA ...
9
Detection of abl/bcr Fusion Gene in Patients Affected by Chronic Myeloid Leukaemia by Dual-Colour Interphase Fluorescence in situ Hybridisation
... and hybridisation and detection conditions in interphase nuclei, the probe was initially hybridised to 5 uncultured lymphocyte preparations from normal ...
5
The use of fluorescence in situ hybridisation techniques in the diagnosis and prognosis of malignancy
... The m aterials section gives the sources of all the DNA probes used, p lu s details of the acquisition of cell lines and tum our sam ples. T he m ethods cover three m ain areas. Firstly, m olecular biological m ethods ...
205
In situ hybridisation: principles and applications
... the detection of various infective agents particularly CMV, HPV, HIV, JC virus, B 19 HSV- 1, EBV, HBV, hepatitis delta virus, Chlamydia trachomatis, salmonella and mycoplasma in tissue ...Besides detection ...
8
Detection of grapevine fenleaf virus (GFLV) in infected grapevines by non-radioactive nucleic acid hybridisation
... GFLV detection by means of nucleic acid hybridisation has been simplified by RNA mini-preparation and slot blot procedure, virus detection with ELISA is still less ...and hybridisation assay ...
6
Somatic hybridisation in different Cyclamen species
... Discussion 123 chromosomes) has not been published so far and the absolute DNA content of diploid C. persicum cultivars (2n=48 chromosomes) was described by Borchert et al., 2007 with 3.21 ± 0.003 pg/ 2C. The relative ...
166
Hybridisation of plasmonic and acoustic biosensing devices
... 31 Biosensors can also be classified into label-free and label-based. Label-based techniques rely on the specific properties of labels like fluorescence, chemiluminescence, etc. for detecting a particular target. ...
164
Detection of Chromosomal Abnormalities with Different In Situ Hybridisation Techniques – the Usefulness in the Qualification of Cancer Patients for Molecularly-Targeted Therapies
... early detection of genetic disorders that are responsible for the stimulation of uncontrolled cancer cells proliferation makes it possible to select a group of patients with a high probability of response to ...
9
Simultaneous and independent detection of C9ORF72 alleles with low and high number of GGGGCC repeats using an optimised protocol of Southern blot hybridisation
... Southern hybridisation-based methods are confounded by the somatic heterogeneity commonly present in blood samples, which might cause false-negative or ambiguous ...
6
hybridisation
... Since the Lewis Structure of NH 3 shows a lone pair on the nitrogen (which resides in one of the hybrid orbitals), the orbital diagram should likewise show a pair of electrons (represen[r] ...
12
Hybridisation for Performance and Economy
... - Electric machine provides torque assist to smaller engine - Electricity generation used to increase load and store. surplus energy for later use[r] ...
14
The genetics of hybridisation in the temperate eucalyptus
... to hybridisation in the genus and as well as the genetic behavior of hybrid ...generation hybridisation is one means of overcoming the constraints and costs of poor clonal propagation and F 1 hybrid seed ...
12
Fluorescence in situ hybridisation (FISH)
... FISH is often performed alongside standard microscope analysis if your child has characteristics that are strongly suggestive of a particular deletion syndrome or another syndrome for [r] ...
8
Hybridisation of social networking and learning environments
... StudyNet is used by people with no interest in existing social networking sites (as an example, the ‘University of Hertfordshire’ network on Facebook has just over 10,000 members, whe[r] ...
8
Array Comparative Genomic Hybridisation (CGH)
... If the genetic change seen in your child is not found in the mother or father then it is likely that this change is the cause of your child’s clinical problems.. Final Report.[r] ...
8
The hybridisation of Russian non-profit organisations
... With limited access to both industrial and governing elites, independent eNPOs had little access to resources that could be secured through such networks and this further entrenched the disparities and inconsistencies ...
16