Hybridization and detection
Analysis of sensitivity and rapid hybridization of a multiplexed Microbial Detection Microarray
... rapid detection of many viruses and bacteria. Pathogen detection microarrays have been used to diagnose viral and bacterial infections in clinical samples and to evalu- ate the safety of biological drug ...
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Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens
... The detection limit was 100 fg of DNA per reaction for the Candida species and 1 pg for the Aspergillus ...of hybridization was assessed with dilution series of the amplification products, using three ...
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Optimizing comparative genomic hybridization probes for genotyping and SNP detection in Plasmodium falciparum
... influenced hybridization to be ...SNP detection, and will facilitate the production of an openly available platform designed for unbiased, genome-wide surveys of polymorphisms optimized for SNP ...
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DNA LABELING, HYBRIDIZATION, AND DETECTION (Non-Radioactive)
... radioactivity is used, autoradiography using X-ray film is employed to visualize the hybrid positions. When chemically labeled probes are used, colorimetric reactions are most often use[r] ...
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An Electrochemical DNA-Hybridization Assay for Acinetobacter baumannii Detection
... the hybridization of DNA, 3 strategies were used in the present study, and their performances were ...for hybridization. The preheated solution hybridization method was conducted by soaking the ...
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Nucleic acid hybridization for detection of cell culture amplified adenovirus
... Probes pAd2-F (containing the fiber gene from Ad2) and pAdl6-BD (containing the BamHI D fragment from Adl6) hybridized only with reference serotypes from the homologous subgroups (C and [r] ...
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Novocastra Fluorescein-conjugated probes for in situ hybridization ISH Detection Kit
... The kit can be used in conjunction with the Novocastra range of fluorescein-conjugated probes. Sufficient reagents for the in situ hybridization staining of 100 preparations are provided. The hybridization ...
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Synthesis and Characterization of a Dye Sensitizing Molecule for Photoelectrochemical DNA Hybridization Detection
... 1.1 Introduction All human cells have a nucleus that contains a person’s genetic fingerprint known as DNA. Three billion chemical bases make up human DNA forming an estimated 50,000 – 100,000 genes in what is called the ...
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Reliability of the RNA-DNA Filter Hybridization for the Detection of Oncornavirus-Specific DNA Sequences
... The RNA eluted from leukemic DNA filters and rehybridized with an excess of normal chicken DNA also shows kinetics of hybridiza- tion that are similar to those of AMV-RNA not prehybridiz[r] ...
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Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture
... The abilities of each of four diagnostic tests-direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybri[r] ...
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Evaluation of DNA colony hybridization and other techniques for detection of virulence in Yersinia species
... This study also revealed new information about the rela- tionship of the plasmid to virulence, first by demonstrating that no bacterium of an avirulent species or biotype carried DNA tha[r] ...
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Problems in detection of cytomegalovirus in urine samples by dot blot hybridization
... Hybridization (with both probes) and conventional meth- ods (virus isolation and detection of early nuclear protein) yielded concordant results with 98 samples; 83 samples were negative [r] ...
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Original Article Detection of cutaneous protothecosis by fluorescence in situ hybridization
... 1 Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China; 2 Department of Dermatology, Center Hospital of Minhang District, Shanghai, PR China. * Equal contributors. Received July 23, 2016; ...
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Detection of the oyster parasite Bonamia ostreae by fluorescent in situ hybridization
... situ hybridization (ISH) is a powerful complement to traditional and PCR-based detection ...Successful hybridization provides an unambiguous phylogenetic confirmation that a specific pathogen is ...
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Detection of Ureaplasma urealyticum by PCR and Biovar Determination by Liquid Hybridization
... liquid hybridization assays in our laboratory were run at 50 and ...with detection of ...liquid hybridization it was possi- ble to detect a mixed infection ...
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Molecular Detection and Identification of Influenza Viruses by Oligonucleotide Microarray Hybridization
... A collection of 476 influenza virus-specific oligonucleotides was spotted onto glass slides as probes. Viral RNAs were reverse transcribed and amplified by PCR, and the products were labeled with cyanine dyes. The ...
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Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization
... DNA-DNA hybridization. The DNA-DNA hybridization enables unambiguous detection of target sequences regardless of the possible presence of nonspecific DNA products and allows the use of PCR primers ...
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Species specific detection of Legionella pneumophila in water by DNA amplification and hybridization
... pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide.. After 35 cycles of amplification, a[r] ...
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Detection of Viral DNA Sequences in Adenovirus-Transformed Cells by In Situ Hybridization
... cell, assuming that cRNA represents 20% of the viral genome, with 10% efficiency for in situ hybridization (10) and 10% for autoradiographic detection (14).. Our results with clonal line[r] ...
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Detection of Epstein Barr Virus in Nasopharyngeal Carcinoma in Sudanese by in Situ Hybridization
... Using ISH for EBER, similar studies were conducted in regions with intermediate endemicity for NPC such as Italy, Spain, the United States of America, Morocco, Iran and Turkey. Interestingly, these studies revealed high ...
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