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Hybridization assay

PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis

PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis

... PCR-reverse blot hybridization assay. The REBA Sepsis-ID test was performed according to the standard protocols provided by the manufac- turer (M&D). For PCR-REBA, hybridization of the PCR ...

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Specific Detection and Identification of Herpes B Virus by a PCR Microplate Hybridization Assay

Specific Detection and Identification of Herpes B Virus by a PCR Microplate Hybridization Assay

... PCR-microplate hybridization assay for the detection and identification of PCR products derived from herpes B virus; this assay is able to detect herpes B virus but not ...The assay will be ...

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The Pneumoplex Assays, a Multiplex PCR Enzyme Hybridization Assay That Allows Simultaneous Detection of Five Organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and

The Pneumoplex Assays, a Multiplex PCR Enzyme Hybridization Assay That Allows Simultaneous Detection of Five Organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and Its Real Time Counterpart

... PCR-enzyme hybridization assay (the standard assay) and a multiplex real-time assay to detect the most common atypical pathogens in a single ...standard assay demonstrated that the ...

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Dot blot hybridization assay of B19 virus DNA in clinical specimens

Dot blot hybridization assay of B19 virus DNA in clinical specimens

... A total of 81 specimens gave reactions in the dot blot hybridization assay that were considered weakly positive, i.e., equal to or less than the reaction given by the 100-pg B19 DNA cont[r] ...

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Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay

Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay

... blot hybridization assay was developed to detect a variety of Entamoeba species and genetic variants known to infect ...The assay was performed after amplification with general Entamoeba-specific ...

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Monoclonal antibody solution hybridization assay for detection of human immunodeficiency virus nucleic acids

Monoclonal antibody solution hybridization assay for detection of human immunodeficiency virus nucleic acids

... This monoclonal antibody solution hybridization assay was compared with an antigen-capture immunoassay for the detection of human immunodeficiency virus in 436 cell culture samples from [r] ...

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“Spoligoriftyping,” a Dual Priming Oligonucleotide Based Direct Hybridization Assay for Tuberculosis Control with a Multianalyte Microbead Based Hybridization System

“Spoligoriftyping,” a Dual Priming Oligonucleotide Based Direct Hybridization Assay for Tuberculosis Control with a Multianalyte Microbead Based Hybridization System

... N ew-generation high-throughput multiplexing instruments constitute a technological breakthrough that allows a change in biological assay design to miniaturization and point-of-care assays (3, 3a, 11, 13, 37, 39). ...

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Monoclonal antibody solution hybridization assay for detection of mouse hepatitis virus infection

Monoclonal antibody solution hybridization assay for detection of mouse hepatitis virus infection

... These results indicate that the MASH method can detect MHV excretion in naturally exposed mice and could be used to diagnose a shedding mouse population either by testing fecal samples f[r] ...

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Detection of Human Herpesvirus 6 DNA in Serum by a Microplate PCR Hybridization Assay

Detection of Human Herpesvirus 6 DNA in Serum by a Microplate PCR Hybridization Assay

... Primary infection with human herpesvirus 6 (HHV-6) causes exanthem subitum (ES), which is characterized by fever lasting approximately 3 days followed by a rash (3, 41). Seropreva- lence among the population is greater ...

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Dot hybridization assay for distinction of rotavirus serotypes

Dot hybridization assay for distinction of rotavirus serotypes

... dsRNAs obtained from 15 stool specimens from the number of Venezuelan infants with diarrhea were dotted in quadruplicate membranes, which were hybridized to probes prepared from D x RRV [r] ...

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An Electrochemical DNA-Hybridization Assay for Acinetobacter baumannii Detection

An Electrochemical DNA-Hybridization Assay for Acinetobacter baumannii Detection

... the hybridization of DNA, 3 strategies were used in the present study, and their performances were ...for hybridization. The preheated solution hybridization method was conducted by soaking the ...

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Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time resolved fluorescence based hybridization assay

Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time resolved fluorescence based hybridization assay

... In this assay format, the PCR is run by standard procedures and the subsequent hybridization reaction is carried out in solution by using two oligonucleotide probes, one biotinylated and[r] ...

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Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166

Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166

... We developed an accurate nonradioactive colony hybridization assay NCHA using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the ide[r] ...

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cDNA probes for the diagnosis of bovine torovirus (Breda virus) infection

cDNA probes for the diagnosis of bovine torovirus (Breda virus) infection

... Cross-hybridization experiments were performed to select Berne virus cDNA clones for use as probes in a dot hybridization assay; the objective was to detect heterologous torovirus RNA in[r] ...

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Comparison of the Second Generation Digene Hybrid Capture Assay with the Branched DNA Assay for Measurement of Hepatitis B Virus DNA in Serum

Comparison of the Second Generation Digene Hybrid Capture Assay with the Branched DNA Assay for Measurement of Hepatitis B Virus DNA in Serum

... quantitative assay for clinical use remains to be ...solution hybridization assay, the Digene Hybrid Capture II assay (HCII), and compared it with another widely used solution ...

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Detection of colonization factor antigen I positive enterotoxigenic Escherichia coli with a cloned polynucleotide probe

Detection of colonization factor antigen I positive enterotoxigenic Escherichia coli with a cloned polynucleotide probe

... We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli ETEC expressing colonization factor a[r] ...

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Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

... mRNA hybridization signal in dying plant ...situ hybridization of catalase ( CAT ), diamine oxidase ( DAO ) and arginine decarboxylase ( ADC ) mRNA transcripts in developing Scots pine seeds and were faced ...

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Quantitative Detection of Hepatitis B Virus by Transcription Mediated Amplification and Hybridization Protection Assay

Quantitative Detection of Hepatitis B Virus by Transcription Mediated Amplification and Hybridization Protection Assay

... HBV quantitative test. The assay consists of proprietary TMA of target DNA (12, 13) and HPA for detection (1). TMA was originally designed for RNA amplification, but the protocol (heat denaturation of the target ...

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Detection and Identification of EightTrichinella Genotypes  by Reverse Line Blot Hybridization

Detection and Identification of EightTrichinella Genotypes by Reverse Line Blot Hybridization

... RLB assay (11, 27) to identify and differ- entiate eight different Trichinella genotypes based on a single PCR ...RLB assay is based on variations in the DNA sequences between 5S rDNA genes within the genus ...

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Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology

Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology

... comet assay were developed subse- quently ...to hybridization sensitivity and reproducibility [44] and the proportion of DNA elements from specific chromosomal domains in comet heads and tails corre- ...

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