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Hybridization, Plant

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections

... of hybridization, and probe con- ...healthy plant control samples and sense probe (which should not hybridize to the target RNA ...healthy plant samples treated with either ...the ...

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Male sterility and somatic hybridization in plant breeding Androesterilidad e hibridación somática en el mejoramiento vegetal

Male sterility and somatic hybridization in plant breeding Androesterilidad e hibridación somática en el mejoramiento vegetal

... somatic hybridization (17, 21, 34, 56, 60, ...intergeneric hybridization followed by several backcrosses (5) and it is incorporated into breeding programs using protoplast fusion experiments ...somatic ...

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Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

... mRNA hybridization signal in dying plant ...situ hybridization of catalase ( CAT ), diamine oxidase ( DAO ) and arginine decarboxylase ( ADC ) mRNA transcripts in developing Scots pine seeds and were ...

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Genetic, morphological, and chemical patterns of plant hybridization

Genetic, morphological, and chemical patterns of plant hybridization

... that hybridization was an ephemeral phenomenon with little importance, reinforcing the ideas of special creation of species prevailing at that time (Roberts ...all hybridization experiments were conducted ...

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Asymmetric Somatic Plant Hybridization: Status and Applications

Asymmetric Somatic Plant Hybridization: Status and Applications

... sexual hybridization related prezygotic or postzygotic barriers ...somatic hybridization to transgenic approaches, somatic hybridization enables broadening of the germplasm base, allows the transfer ...

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Genetic Analysis of Some Metric Plant Traits in Upland Cotton (Gossypium hirsutum L ) through Hybridization

Genetic Analysis of Some Metric Plant Traits in Upland Cotton (Gossypium hirsutum L ) through Hybridization

... per plant indicated that there were additive type of gene action controlling these traits as the value of D was greater than H1 and ...per plant showed the over dominance type of gene action proved by the ...

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Breeding Strategies for Improving Rice Yield—A Review

Breeding Strategies for Improving Rice Yield—A Review

... 5.1. Diversifying the CMS System to Improve the Sustainability of Hybrid Rice Technology Cytoplasmic male sterility (CMS) resulting from nuclear-cytoplasm interaction has been commercially exploited for the production of ...

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Comparative Genomics of Host-Specific Virulence in Pseudomonas syringae

Comparative Genomics of Host-Specific Virulence in Pseudomonas syringae

... 39 plant hosts by DNA microarray-based comparative genomic hybridization against an array containing 353 virulence-associated (VA) genes, including 53 type III secretion system effectors ...

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Biotechnological methods utilized in Cucumis research – A review

Biotechnological methods utilized in Cucumis research – A review

... interspecific hybridization between ...2. Plant formation was detected after intraspecific hybridization within the genus Cu- ...interspecific hybridization between cu- cumber and several ...

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Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).

Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).

... The expression of the target gene was detected by western blotting. The total protein from control and transgenic lettuce plants that showed a hybridization signal in the Southern blot was extracted using a ...

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Transgenic approaches to combating pests and diseases of plants

Transgenic approaches to combating pests and diseases of plants

... distinct plant species are frequently created by classical breeders who deliberately force hybridization between distinct plant species when carrying out interspecific or intergeneric wide crosses ...

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Mapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization.

Mapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization.

... of hybridization of the rhodamine labeled YAC (red) was visualized on DAPI-stained chromosomes (blue) displayed here in false color after aligning and merging the two separate images of the two different ...two ...

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Characterization of Chromosomal Abnormalities in Cancer by Spectral Karyotyping

Characterization of Chromosomal Abnormalities in Cancer by Spectral Karyotyping

... situ hybridization technique (FISH) enabled detection of chromosomal microdeletions and structural abnormalities ...genomic hybridization (17) and spectral karyotyping (SKY) (18) have relatively improved ...

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The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas

The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas

... clinical analysis of HER2 gene amplification status. In the current study, FISH and a chromogen-based in situ hybridization system (SISH) were compared with each other and with each of the antibody assays ...

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Molecular Epidemiology of Oral Treponemes Associated with Periodontal Disease

Molecular Epidemiology of Oral Treponemes Associated with Periodontal Disease

... situ hybridization or dot blot hybridization after prior amplification with eubacterial ...situ hybridization, and their morphologies, sizes, and numbers could be ...

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Amplified Fragment Length Polymorphism Analysis: the State of an Art

Amplified Fragment Length Polymorphism Analysis: the State of an Art

... a plant retrotranspo- son (27, 60, 119) or the IS6110 element of Mycobacteria tuberculosis (34), is used to amplify fragments that carry the respective interspersed ...

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Genotyping of Measles Virus in Clinical Specimens on the Basis of Oligonucleotide Microarray Hybridization Patterns

Genotyping of Measles Virus in Clinical Specimens on the Basis of Oligonucleotide Microarray Hybridization Patterns

... Validation of MV microarray genotyping. In addition, two different blinded panels of samples were evaluated at the Johns Hopkins Bloomberg School of Public Health using microarray slides made at the FDA laboratory. Of ...

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Cellular Localization of Latent Murine Cytomegalovirus

Cellular Localization of Latent Murine Cytomegalovirus

... Immunohistochemistry. Immunohistochemistry was performed on the tissue section immediately adjacent (contiguous) to the section found positive for the latent MCMV IE-1 DNA by PISH. Sections were deparaffinized and ...

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Interpretation of Mutations, Expression, Copy Number in Somatic Breast Cancer: Implications for Metastasis and Chemotherapy

Interpretation of Mutations, Expression, Copy Number in Somatic Breast Cancer: Implications for Metastasis and Chemotherapy

... for hybridization studies in any genomic region is finite, and in some instances, underrepresented in regions associated with disease or relevant to gene regulation and ...with hybridization kinetics ...

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Model based design of RNA hybridization networks implemented in living cells

Model based design of RNA hybridization networks implemented in living cells

... RNA hybridization networks might be very use- ful to perform bio-logical computations in living cells, due to their ability for storing and retrieving information ...RNA hybridization networks might be ...

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