hybridization technique
Use of the checkerboard DNA DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L )
... DNA-DNA hybridization technique [5-8] is a semi-quantitative technique that has been extensively employed in odontology to detect and quan- tify a variety of bacterial species in dental samples and ...
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Detection of herpes simplex virus DNA from cerebrospinal fluid by PCR and a rapid, nonradioactive hybridization technique
... A molecular assay for the detection of herpes simplex virus HSV, including a novel, nonradioactive hybridization technique, was evaluated with a total of 123 cerebrospinal fluid specimen[r] ...
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Evaluation of fluorescent in situ hybridization technique for diagnosis of malaria in Ahero Sub County hospital, Kenya
... the technique invaluable in such settings, which characterize many sub-Saharan African countries ...the technique expen- sive in terms of acquisition of microscopes and also the high cost of maintenance ...
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Molecular Genetic study to detect Numerical Aberration of Cyclin D1 (CCND1) Gene by using Fluorescence in Situ Hybridization technique as a diagnostic and prognostic tool in Oral Squamous Cell Carcinoma
... A Leica DM2500 Fluorescence microscope equipped with 10x, 20x, 40x dry, and 100x oil immersion objectives with triple-pass filter for spectrum Green/Spectrum Orange and DAPI (Vysis) was used to count the fluorescent ...
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DETECTION OF HUMAN PAPILLOMAVIRUS USING IN SITU HYBRIDIZATION TECHNIQUE IN VULVO-VAGINAL WARTS
... The results of in situ hybridization are shown in Table I. The staining pattern with specific biotinylated DNA was observed as dense and homogenous, blackish purple nuclear stain, predominantly located in the ...
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Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria
... capture hybridization technique 2 the best choice as a molecular test for the detection and identification of HPV-HR and best suited in unstructured ...the technique is fast, reproducible and ...
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<p>Human papillomavirus DNA in surgical smoke during cervical loop electrosurgical excision procedures and its impact on the surgeon</p>
... In addition, as determined by the fl ow fl uorescence in situ hybridization technique, the prevalence rates of HPV in this study were 94.8% (127/134) in cervical cells, 69.4% (40/ 134) in surgical smoke and ...
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ASSIGNMENT OF THREE GENE LOCI (PGK, HGPRT, G6PD) TO THE LONG ARM OF THE HUMAN X CHROMOSOME BY SOMATIC CELL GENETICS
... Using the hybridization technique with the KOP cell line and employing HAT medium, which selects for the gene for HGPRT (LITTLEFIELD 1966), it should be possible to assign the X-linke[r] ...
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Screening for hepatitis B virus in healthy blood donors by molecular DNA hybridization analysis
... A DNA molecular hybridization technique employing a purified adw subtype hepatitis B virus HBV cloned DNA of 3.2 kilobase pairs as a probe was used to screen for the presence of HBV DNA [r] ...
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Chromosomal localization of the genes for the vitronectin and fibronectin receptors alpha subunits and for platelet glycoproteins IIb and IIIa
... situ hybridization technique, we have determined the chromosomal location of the genes for the alpha subunits of the vitronectin receptor (VNR alpha), the fibronectin receptor (FNR alpha), and for the alpha ...
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Molecular probe for identification of Trichomonas vaginalis DNA
... vaginalis DNA fragment that was used as a probe for the detection of the protozoans in vaginal secretions by a dot blot hybridization technique.. MATERIALS AND METHODS.[r] ...
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Characterization of Chromosomal Abnormalities in Cancer by Spectral Karyotyping
... situ hybridization technique (FISH) enabled detection of chromosomal microdeletions and structural abnormalities ...genomic hybridization (17) and spectral karyotyping (SKY) (18) have relatively ...
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Nucleic acid hybridization for detection of cell culture amplified adenovirus
... Seven cloned probes, as well as total adenovirus type 2 Ad2 and Adl6 genomic DNA, were tested by a nucleic acid hybridization technique for sensitivity and specificity in detecting adeno[r] ...
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Molecular Epidemiology of Oral Treponemes Associated with Periodontal Disease
... situ hybridization or dot blot hybridization after prior amplification with eubacterial ...situ hybridization, and their morphologies, sizes, and numbers could be ...
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Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes
... intracellulare, hybridization results from the BACTEC material for the 190 cultures were derived by dividing the hybridization value obtained with the most reactive probe by the average [r] ...
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Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells
... Simple Genetic Method To Identify Viridans Group Streptococci by Colorimetric Dot Hybridization and Fluorometric Hybridization in Microdilution Wells TAKAYUKI EZAKI,* YASUHIRO HASHIMOTO,[r] ...
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Cellular Localization of Latent Murine Cytomegalovirus
... The hybridization buffer (consisting of 50% formamide, 43 SSC [13 SSC is ...the hybridization buffer was saved for the detection of diffused product DNA and DNA-probe ...
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Multiplexed automated digital quantification of fusion transcripts: comparative study with fluorescent in-situ hybridization (FISH) technique in acute leukemia patients
... Complete karyotype analysis at diagnosis was performed on all leukemic patients and followed a standardized la- boratory protocol. Banded chromosomes (20 metaphases) were examined for each patient sample utilizing the G- ...
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New techniques to detect genomic variation
... the technique has increased from the 5-10 Mb level towards the kilobase ...each technique has specific applications, and which technical approach to use depends on the resolution and throughput of the ...
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Osteopontin mRNA is expressed by smooth muscle derived foam cells in human atherosclerotic lesions of the aorta
... osteopontin mRNA was detected in 24 out of 25 samples of aorta obtained from 17 autopsy cases, but not in one normal aortic sample. The magnitude of expression was proportional to the stage of atherosclerosis. In situ ...
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