The identification and strain typing of A. xylosoxidans (Chapter 3)
SNIT: SNP identification for strain typing
... The efficiency of the SNIT pipeline stems from the use of pairwise alignments based on exact matches. However, this approach limits the application of the pipeline to bacterial and eukaryotic genomes. Due to the high ...
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MOLECULAR AND BIOCHEMICAL IDENTIFICATION OF ACHROMOBACTER XYLOSOXIDANS ISOLATED FROM CYSTIC FIBROSIS PATIENTS IN AL–DIWANIYA CITY
... A. xylosoxidans tends to establish itself in lungs already infected by ...A. xylosoxidans was co– colonized by ...A. xylosoxidans to infect lungs that are not previously ...A. xylosoxidans ...
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Identification of a Novel DNA Probe for Strain Typing Mycobacterium bovis by Restriction Fragment Length Polymorphism Analysis
... Molecular typing methods such as restriction fragment length polymorphism (RFLP) analysis and spoligotyping have been used to identify related herd breakdowns in an attempt to identify more precisely the route of ...
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Proteomic identification of Axc, a novel beta-lactamase with carbapenemase activity in a meropenem-resistant clinical isolate of Achromobacter xylosoxidans
... A 65-year old patient, diagnosed with chronic lymphocytic leukemia in 1989, underwent a non-myeloablative stem cell transplantation in July 2014. The patient was discharged on July 25 th but readmitted on August 1 st ...
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Molecular Methods for Bacterial Strain Typing
... bacterial typing techniques is used that are different from one another in the aspects of study objectives, costs, reliability and discriminatory ...the typing methods can achieve all desired objectives of ...
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Polymorphic Amplified Typing Sequences Provide a Novel Approach to Escherichia coli O157:H7 Strain Typing
... the typing of ...for typing termed PATS, in which we directly tested for the presence or absence of the DNA segments containing each individual polymorphic XbaI ...their identification would help ...
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Bacterial Infections Caused by Achromobacter xylosoxidans
... and Identification of Bacteria : All specimens were examined microscopically and plated on several agar media, including MacConkey agar and Blood agar, plates, and incubated under , aerobic and anaerobic at 37°C ...
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Evaluation of the Polymorphisms Associated with Tandem Repeats for Pseudomonas aeruginosa Strain Typing
... Data analysis. Band size estimates were exported from the Bionumerics soft- ware and converted to numbers of units. The resulting data were imported back into Bionumerics software for use for clustering analysis with the ...
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Comparative genomic approaches to understanding Achromobacter xylosoxidans
... A. xylosoxidans were based on phenotypic or biochemical tests (Yabuuchi & Oyama, ...the identification of clinical isolates, 16S rDNA sequence shows the limitation to identify ambiguous clinical ...
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Comparison of Strain Typing Results for Clostridium difficile Isolates from North America
... presumptive identification of an isolate by the Xpert ...the strain type would require culturing of the stool sample to recover the ...for typing using one of the three methods discussed ...for ...
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Vibrio cholerae Strain Typing and Phylogeny Study Based on Simple Sequence Repeats
... bacterial identification and typing is essen- tial for several disciplines, including taxonomy, epidemiology, determining phylogenetic relationships, and the study of evo- lutionary ...microorganism ...
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New Microsatellite Multiplex PCR for Candida albicans Strain Typing Reveals Microevolutionary Changes
... the typing of 72 unrelated clinical isolates, the analysis revealed that they were all polymorphic, presenting from 5 to 30 alleles and 8 to 46 different ...for strain relatedness were identified: (i) ...
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Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review
... .Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population ...exact typing (PCR based methods, microstallite length polymorphism, ...
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Molecular Strain Typing of Clinical Isolates,
... frequent strain was TRS-1, type-1 and found in all the four quadrants of Chennai city and also in the neighbouring regions ...type-I strain was predominant in whole of Chennai and neighbouring ...Type-III ...
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DNA probe for strain typing of Cryptococcus neoformans
... fingerprints of two Australian isolates (B-4506 and B-4507) obtained from the debris of Eucalyptus trees 15 km apart in the same valley were identical to each other while being different[r] ...
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Characterization of IS1245 for Strain Typing of Mycobacterium avium
... same strain, as defined by the combination of epidemiologic and PFGE data, often demon- strated appreciable ...a strain have indistinguishable IS1245 patterns; 4 (13%) strains included isolates whose IS1245 ...
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Evaluation of the Tuberculosis Strain Typing Service in England
... TB strain typing services have been introduced in other countries, 100,133,136,152 but the knowledge, perceived usefulness and practices of users have not been ...of strain typing on contact ...
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Serological classification of Achromobacter xylosoxidans
... If antibody titers for the homologous strain remained constant and antibody titers for the test strain and the heterologous strain were reduced,.. this strain was determined to be a sero[r] ...
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Whole Genome Mapping: a New Paradigm in Strain Typing Technology
... detect important structural changes and mobile genetic motifs that can be missed even by next-generation sequencing. ACCESSING THE TECHNOLOGY WGM has been available for over 10 years, but its move from the research ...
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Direct identification and typing of Mycobacterium tuberculosis by PCR
... rapid, strain-specific typing of clinical isolates for epidemiolog- ical purposes, including patient reinfection studies and detec- tion of laboratory ...for typing of ...
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