Immediate-Early Gene Results
SRF activation in immediate early gene transcription
... reporter gene between single cells as well as the whole population of cells following different ...that results obtained using total cell lysates or total RNA represents an average signal from the cell ...
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The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression
... On the basis of these preliminary findings, we conducted a comparative study of viral replication rates after normalizing input viral titers by two independent methods that circumvent potentially deceptive plaque assay ...
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Cell Cycle-Independent Expression of Immediate-Early Gene 3 Results in G1 and G2 Arrest in Murine Cytomegalovirus-Infected Cells
... CMV immediate-early gene expression and the cell cycle position of the host ...in early (G 1 /S) and late (G 2 ) cell cycle ...the early ie3-mediated cell cycle block is unknown, but in ...
11
The Major Immediate-Early Gene ie3 of Mouse Cytomegalovirus Is Essential for Viral Growth
... The IE3 protein expressed by the complementing cell line was sufficient to allow growth of the ie3 mutants. However, the titers of the ie3 mutants obtained on the cell line did not reach those levels which were achieved ...
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Infection of Cells with Human Cytomegalovirus during S Phase Results in a Blockade to Immediate-Early Gene Expression That Can Be Overcome by Inhibition of the Proteasome
... IE gene products. These results were obtained with both Abs that detect domains that are common to IE86 and IE72 and Abs specific for the C terminus of ...IE gene expression requires some factor that ...
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The Human Cytomegalovirus Major Immediate-Early Enhancer Determines the Efficiency of Immediate-Early Gene Transcription and Viral Replication in Permissive Cells at Low Multiplicity of Infection
... on gene transcrip- tion, we infected cells at an either high (approximately 5 PFU/ cell) or low ...Similar results were obtained with the antisense IE2 riboprobe (data not ...an early viral promoter ...
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The C-Mer Gene Is Induced by Epstein-Barr Virus Immediate-Early Protein BRLF1
... these results indicate that host cells containing the lytic form of EBV infection may have a greatly enhanced re- sponse to the Gas6 ...arrest gene, is expressed constitu- tively by a number of cell types ...
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Construction and properties of pseudorabies virus recombinants with altered control of immediate-early gene expression.
... essential immediate-early (IE) gene of pseudorabies virus influences virus replication and virulence, we replaced the IE promoter with the tissue-specific promoters of the bovine cytokeratin IV ...
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Requirement of the immediate early gene vesl-1S/homer-1a for fear memory formation
... Results: In this study, we generated short-form-specific gene targeting (KO) mice by knocking in part of vesl-1L/ homer-1c cDNA. Homozygous KO mice exhibited normal spine number and morphology. Using the ...
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Rat Cytomegalovirus Major Immediate-Early Enhancer Switching Results in Altered Growth Characteristics
... major immediate-early (MIE) enhancer of cytomegalovirus (CMV) is important in determining virus tropism and latency because of its essential role in initiating the cascade of early gene ...
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Inhibition of Human Cytomegalovirus Immediate-Early Gene Expression by Cyclin A2-Dependent Kinase Activity
... IE gene expression as the critical first step of the HCMV lytic cycle is under the control of cell type-, cell differen- tiation-, and cell cycle-dependent mechanisms that are poorly un- derstood at the molecular ...
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Identification of an Intercistronic Internal Ribosome Entry Site in a Marek's Disease Virus Immediate-Early Gene
... these results that the EMCV IRES had strong activity compared with the ICR IRES from ...These results indicated that the ICR IRES had the ability to internally initiate translation in a cap-inde- pendent ...
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Inhibition of Transcription of the Beta Interferon Gene by the Human Herpesvirus 6 Immediate-Early 1 Protein
... These results suggest that in the pres- ence of IE1, IFN-  gene transcription is rapidly turned off or, alternatively, that IE1 causes the premature degradation of IFN-  ...
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Autoregulation of the human cytomegalovirus major immediate-early gene.
... HF COS Inf. Expression of pSVCC2 in COS-1 cells. COS-1 cells were transfected with pSVCC2 as described in Materials and Methods. HF) and cytoplasmic RNA (25 ,g) (TF COS) from transfected[r] ...
7
An early gene maps within and is 3' coterminal with the immediate-early gene of equine herpesvirus 1.
... h postinfection; no metabolic inhibitors) conditions, in addition to the 6.0-kb IE mRNA, a 4.4-kb early (E) mRNA is transcribed from the IE gene region beginning at approximately 4 h pos[r] ...
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Structure and expression of murine cytomegalovirus immediate-early gene 2.
... Transac- tivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IEl: mutational analysis of the viral proteins. Inserti[r] ...
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Nucleotide sequence of an immediate-early frog virus 3 gene.
... R-loop mapping of the cloned XbaI K fragment of frog virus 3 DNA with immediate-early RNA from infected cells showed that an RNA of approximately 500 to 600 nucleotides (the right size t[r] ...
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Structural analysis of the major immediate early gene of human cytomegalovirus.
... It is hypothesized that this viral gene codes for the major regulatory protein controlling transcription of the viral genome at early times.. The properties of the viral gene and its pro[r] ...
10
Repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus.
... Our ap- proach was to coinfect cells at the nonpermissive tempera- ture with wild-type adenovirus and a temperature-sensitive mutant in the immediate-early gene of either pseudorabies vi[r] ...
5
Sequence and structural organization of murine cytomegalovirus immediate-early gene 1.
... Also, after comparing maintain a high degree of sequence conservation in certain the protein sequences, only one region with homology regions of the polypeptide, including amino acids 67[r] ...
8