Immediate-early protein
The Immediate-Early Protein IE0 of the Autographa californica Nucleopolyhedrovirus Is Not Essential for Viral Replication
... The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant ...
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A Herpesviral Immediate Early Protein Promotes Transcription Elongation of Viral Transcripts
... an immediate early protein, we also used immunofluorescence to visualize the localization of SSRP1 early in ...of immediate early proteins (Table S1), SSRP1 is redistributed ...
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The Human Cytomegalovirus 86-Kilodalton Major Immediate-Early Protein Interacts Physically and Functionally with Histone Acetyltransferase P/CAF
... major immediate-early proteins of human cytomegalovirus (HCMV) play a pivotal role in controlling viral and cellular gene expression during productive ...major immediate early protein ...
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Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency.
... The protein encoded by ORF-63 has homology with ICP-22 of HSV-1, whose functions are not very well characterized (29, 30, ...ORF-63 protein in VZV have begun to be clarified, showing evidence for down ...
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Phosphorylation of the Human Cytomegalovirus 86-Kilodalton Immediate-Early Protein IE2
... In a variety of experiments (not shown), we have noted that the migration of IEP86 on denaturing polyacrylamide gels is variable depending on the source of its production. For exam- ple, IEP86 produced in human cells ...
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Binding of the pseudorabies virus immediate-early protein to single-stranded DNA.
... Results of early studies have shown that ICP4 from infected cell extracts binds to single-stranded DNA-cellulose columns 6.. Wilcox et al 17 have reported that ICP4 exists in several pho[r] ...
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The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription.
... To determine whether the viral IE protein constitutively expressed in L cells could stimulate expression of the CAT gene under control of a eucaryotic promoter, L cells and 45/1 cells we[r] ...
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Reduced Expression of the Immediate-Early Protein IE0 Enables Efficient Replication of Autographa californica Multiple Nucleopolyhedrovirus in Poorly Permissive Spodoptera littoralis Cells
... Infection of Spodoptera littoralis SL2 cells with the baculovirus Autographa californica multiple nucleopoly- hedrovirus (AcMNPV) results in apoptosis and low yields of viral progeny, in contrast to infection with S. ...
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Multimerization of ICP0, a herpes simplex virus immediate-early protein.
... To determine whether any of the transactivation-deficient IE-0 mutants that we isolated displayed a dominant negative phenotype, Vero cells were cotransfected with equimolar amounts of a[r] ...
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Gammaherpesvirus Lytic Gene Expression as Characterized by DNA Array
... binding protein; GP B, glycoprotein B; THYM KIN, thymidine kinase; U-DNA GLYCOS, uracil DNA glycosylase; DNA REP PROT, DNA replication protein; IE PROT, immediate-early protein; IL-8 R ...
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The ORF45 Protein of Kaposi's Sarcoma-Associated Herpesvirus Is Associated with Purified Virions
... an immediate-early gene product which is responsible for inhibiting IFN antiviral response (22, ...an immediate-early protein, suggest that ORF45 plays important roles in viral life ...
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Dynamin Is Required for Efficient Cytomegalovirus Maturation and Envelopment
... at early time points, which directly correlate with virus entry, are ...lower protein levels throughout the infection, which does not seem to be the case ...on early viral gene expression or abnormal ...
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trans activation of an immediate-early frog virus 3 promoter by a virion protein.
... The immediate-early genes are transcribed from parent templates even in the presence of cycloheximide; the lack of a requirement for de novo protein synthesis indicates either that this [r] ...
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Characterization of a Human Cytomegalovirus with Phosphorylation Site Mutations in the Immediate-Early 2 Protein
... major immediate-early locus, including exons 1 to 5 and the ...major immediate-early locus was replaced by a cassette conferring resistance to kanamycin ...IE2 protein, resulting in the ...
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Physical and functional domains of the herpes simplex virus transcriptional regulatory protein ICP4.
... Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.. Activation of immediate-early, early, and[r] ...
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Tegument Protein ORF45 Plays an Essential Role in Virion Morphogenesis of Murine Gammaherpesvirus 68
... each protein was quantitated by densitometric analysis, normalized to that of actin in the same sample, and compared to that from WT BAC, with the expression level of each protein from WT BAC set to ...
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Neural and neuroendocrine processing of a non photic cue in an opportunistically breeding songbird
... Real-time quantitative PCR (qPCR) on cDNA obtained from hypothalamic punches was run for a number of genes: DIO2, DIO3, GnRH, GnIH, and EGR-1, an immediate-early gene (IEG). This IEG was taken as an ...
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Enhancerless Cytomegalovirus Is Capable of Establishing a Low-Level Maintenance Infection in Severely Immunodeficient Host Tissues but Fails in Exponential Growth
... tive immunity, are not completely devoid of innate immunity, which might have led to an early resolution of a low-level infection by the enhancerless virus. However, it still remains to be determined whether the ...
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Phosphorylation and nuclear localization of the varicella-zoster virus gene 63 protein.
... virus immediate-early promoter upstream of the pUC18 mul- tiple cloning site and simian virus 40 polyadenylation se- quences, to create pCMV63 and pCMV63R, the latter containing ORF 63 in the reverse ...
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Temporal Gene Regulation of the Channel Catfish Virus (Ictalurid Herpesvirus 1)
... an immediate-early gene and prospective early and late genes were char- ...third immediate-early transcript was ...the protein specified by open reading frame (ORF) ...
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