Immobilized Lipase
Determination of External Mass Transfer Model for Hydrolysis of Jatropha Oil Using Immobilized Lipase in Recirculated Packed Bed Reactor
... The reaction mixture is prepared in proportion of 15 ml of Jatropha oil, 24 ml of n-hexane, 1.2 ml of water. The mixture is incubated at 40˚C and stirred at 200 rpm. Immobilized lipase is packed into ...
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Immobilized Lipase Technology for Making Natural Flavor
... Natural flavor produced by reacting coconut oil with citronellol / geraniol derived from isolated lemongrass oil. This process is called esterification reaction with immobilized lipase. Esterification is ...
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Enzymatic Synthesis of Butyl Ferulate by Silica Immobilized Lipase in a Non Aqueous Medium
... bound lipase in the presence of molecular sieves, and certain salt ions (Cu 2+ , Zn 2+ and Al 3+ ) enhanced the ester ...an immobilized enzyme with a support which has hydrophilic nature is used, water ...
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Lipase Catalyzed Incorporation of Conjugated Linoleic Acid by Transesterification into Sunflower Oil Applying Immobilized Lipase (Lipozyme Thermomyces lanuginosus and Rhizomucor mehei)
... Conjugated Linoleic Acid (CLA), Glycerol (G) and sunflower oil blends with varying concentration were subjected to enzymatic esterification using a 1, 3- specific immobilized lipase. CLA was used as acyl ...
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Optimization Method for The Synthesis of Fatty Amide Surfactants from Oleic Acid Catalyzed by Immobilized Lipase
... Response Surface Methodology (RSM) is successfully used to obtain the optimum conversion of oleic acid on the synthesis of fatty amide surfactants from oleic acid and diethanolamine by immobilized lipase. ...
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Synthesis of naringin 6"-ricinoleate using immobilized lipase
... The lipase used was immobilized lipase from C. antarctica recombinant in Aspergillus oryzae from Sigma-Aldrich ® . The naringin and molecular sieves (3 Å) were purchased from Sigma-Aldrich ® . The ...
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Kinetics and Isotherm Studies of the Immobilized Lipase on Chitosan Support
... the immobilized lipase and the mechanism of immobilization on chitosan beads and activated chitosan beads with glutaraldehyde were ...of lipase on chitosan beads activated by glutaraldehyde so that ...
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Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil
... its initial conversion efficiency (Fig. 7b). In comparison, a recent report describing the production of biodiesel from WCO using immobilized lipase particles found that the conversion dropped to below 70% ...
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Enzymatic Cellulose Palmitate Synthesis Using Immobilized Lipase
... using immobilized lipase. Lipase can be produced from Mucor miehei, followed by purification and ...of lipase was carried out through precipitation using ammonium sulfate saturation of 20-60% ...
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Covalently immobilized lipase on a thermoresponsive polymer with an upper critical solution temperature as an efficient and recyclable asymmetric catalyst in aqueous media
... cepacia lipase (PSL) using a UCST-type thermoresponsive polymer, poly(acrylamide-co-acrylonitrile) (pAA), as the carrier, and investigate its enantioselective application in an aqueous ...produced ...
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Optimization of biodiesel production from jatropha oil using immobilized lipase
... In this study, PVA was used as the entrapment material since it has been shown to possess desirable characteristics. Due to PVA’s excellent properties, PVA would serves as an excellent material to be applied in the ...
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Investigation of extraction and transesterification of algae by immobilized lipase as biofuel
... In this study, three different methods were investigated to study the disruption of algae cells walls such as ultrasonication, soxhlet and hexane solvent extraction.. and Spirulina sp.[r] ...
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Synthesis and Characteristics of Mesoporous Sol-gels for Lipase Immobilization
... The immobilized lipase showed higher activity at higher temperature (above ...crude lipase is its sensitivity in alkaline conditions, but immobilized enzyme seems to retain its activity in ...
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Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor
... of lipase by adsorption method was carried out by modification according to Omar and Suguna ...of lipase was dissolved in 5 ml phosphate buffer solution of pH ...the immobilized lipase ...
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Application of a Burkholderia cepacialipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude Jatropha oil
... A maximum yield of 90% was attained after 12 hours at the stoichiometric molar ratio of 3:1. Stevenson et al. [57] reported that the final yield of biodiesel could be further increased by adding silica gel, which may ...
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Immobilization of porcine pancreas lipase onto bristles of plastic brush: Kinetic properties
... The immobilized lipase showed maximum activity at 37°C which is higher than that of the free enzyme ...of immobilized enzyme was increased from 20 min to 30 ...of lipase was decreased from 50 ...
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Lipase Catalyzed Methanolysis of Tri (12 Hydroxy Stearoyl) Glycerol in Organic Solvents
... co-solvent on a bed of immobilized lipase [2]. Additionally, by judicious use of solvent and lipase, we have been able to produce high yields of 1,2-diricinoleoyl diacylglycerol [3]. The ...
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Enzymatic transesterification of Jatropha oil
... or immobilized lipases [7,8,10,15,18,21-23]. Immobilized lipase in particular is suitable for continuous biodiesel production because of the ease of its recovery from the reaction ...of ...
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Synthesis of food ester butyl acetate by immobilized Aspergillus oryzae (NCIM 1212)lipase
... Abstract- The production of lipase was carried out by A. oryzae (NCIM 1212) by solid state fermentation using agriculture wastes. An enzyme activity of 6.248 U/ml/min was obtained by pNPP assay. Pineapple flavourd ...
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Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase
... lipolytica lipase was 85% in a 3-hour ...antarctica lipase was only 70% in a 3-hour reaction; 5 hours were needed to reach 85% ...Antarctica-immobilized lipase by a sub- strate of lauryl ...
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