Immunoglobulin G and M
Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections
... Chromatography on controlled pore glass was adapted for the separation of immunoglobulin M IgM and immunoglobulin G IgG rubella antibodies from 0.3-ml samples of human serum.. An extreme[r] ...
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SERUM IMMUNOGLOBULIN G, M AND A LEVELS IN CHILDREN WITH NEPHROTIC SYNDROME AND ITS CORRELATION WITH BIOCHEMICAL PARAMETERS
... unoglobulin M (IgM) before isotype switching to immunoglobulin G (IgG) or immunoglobulin A (IgA), depending on the kind of the antigen (Colucci et ...
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Detection of Antichlamydial Immunoglobulin G and M Antibodies by Enzyme Linked Immunosorbent Assay
... If the ELISA measures the same types of antibodies as detected by the MIF test, then it is not likely to be useful in diagnosing chlamydial infections.. If, however, the ELISA measures d[r] ...
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Enzyme linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera
... At present, the most widely used methods for detection of chlamydial antibodies in hUiman serum are the complement fixation test and various modifications of the microimmunofluorescence [r] ...
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Immunoglobulin G and M specific enzyme linked immunosorbent assay for detection of dengue antibodies
... However, the HI titer and the ELISA value when using a polyspecific conjugate on these samples were more difficult to evaluate, since both methods also demonstrated antibodies in the cor[r] ...
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Serum immunoglobulin G, M and A response to Cryptosporidium parvum in Cryptosporidium HIV co infected patients
... Each well of the 96-well microtitre plate (Nunc Inter Med, Denmark) was coated with 100 μl optimum dilution of the CCA in carbonate bicarbonate buffer. Plates were incubated at 4°C overnight followed by washing thrice ...
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Evaluation of Combined Rapid Immunoglobulin M and Immunoglobulin G Lateral Flow Assays for the Diagnosis of Leptospirosis, Scrub Typhus, and Hantavirus Infection
... diagnosis of leptospirosis, scrub typhus, and hantavirus infection, using archived blood samples from 434 patients with laboratory-confirmed leptospirosis (131) or scrub typhus (128), a[r] ...
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Measurement of immunoglobulin M, immunoglobulin G, and immunoglobulin A antibodies against Yersinia enterocolitica by enzyme linked immunosorbent assay: comparison of lipopolysaccharide and whole bacterium as antigen
... enterocolitica serotypes 0:3 and 0:9, Yersinia pseudotuberculosis IA, and Brucella abortus were studied by human and rabbit antisera in the whole bacterium and lipopolysaccharide ELISAs [r] ...
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Microenzyme Linked Immunosorbent Assay for Detection of Immunoglobulin G and Immunoglobulin M Antibodies to Legionella pneumophila
... These assays were compared with the previously standardized indirect immunofluorescence test in four groups of subjects: i pneumonia patients with culture-proven Legionnaires disease wit[r] ...
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Elimination of nonspecific cytomegalovirus immunoglobulin M activities in the enzyme linked immunosorbent assay by using anti human immunoglobulin G
... Unfortunately, IgM monitoring does not usually discriminate between specific and nonspecific IgM activities, and in the case of CMV infection, an aberrant ratio of specimens displaying s[r] ...
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Recombinant Antigens To Detect Toxoplasma gondii Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay
... Due to the inherent limitations of the tachyzoite antigen in serologic tests, the advent of purified recombinant antigens obtained via molecular biology is an attractive alternative for the detection of serum antibodies. ...
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Persistence of immunoglobulin G and immunoglobulin M antibodies after postnatal rubella infection determined by solid phase radioimmunoassay
... Demonstration of a recent viral infection by the determination of specific immunoglobulin M IgM antibodies is dependent upon knowing the "normal" persistence of these antibodies after an[r] ...
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An enzyme immunoassay for immunoglobulin M antibodies to Toxoplasma gondii which is not affected by rheumatoid factor or immunoglobulin G antibodies
... None of the 22 sera containing high levels of IgM rheumatoid factor RF gave positive ELISA IgM results, even though 8 of them also had high levels of IgG toxoplasma antibodies.. Mixtures[r] ...
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Enzyme linked immunosorbent assay for mumps and parainfluenza type 1 immunoglobulin G and immunoglobulin M antibodies
... The 1: 500 dilution was used for discrimination between positive and negative results, because many normal sera as well as sera containing high titers of rheumatoid factor gave high abso[r] ...
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Indirect enzyme linked immunosorbent assay for immunoglobulin G and four immunoassays for immunoglobulin M to Toxoplasma gondii in a series of heart transplant recipients
... Toxoplasma gondii infections in heart transplant recipients were monitored by indirect enzyme-linked immunosorbent assay for immunoglobulin G ELISA-IgG, indirect ELISA-IgM in serum IgM f[r] ...
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Immunoglobulin G, A, and M Responses to BK Virus in Renal Transplantation
... Immunoglobulin G (IgG), IgA, and IgM antibodies were measured in serum samples from 71 organ donors, 81 kidney transplant recipients at transplantation, and 67 patients during the posttransplant period by ...
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Solid phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies
... Balls adsorbed at each rubella virus protein concentration were incubated with dilutions of a rubella antibody-positive serum and tested for binding of rubella-specific IgG antibodies up[r] ...
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Diagnostic significance of immunoglobulin M antibodies to Toxoplasma gondii detected after separation of immunoglobulin M from immunoglobulin G antibodies
... A serum was obtained 2 DT titer IgG containing 21.5 or 2.7 mg of IgG months after the onset of symptoms and signs per ml and with DT titers of 1:8,096 and 1:1,024, in a patient with toxo[r] ...
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Serological screening of immunoglobulin M and immunoglobulin G during pregnancy for predicting congenital cytomegalovirus infection
... for immunoglobulin (Ig) M is difficult to diagnose as primary infection, because CMV IgM persists for a long time (> 5 months to over 1 year) after infection in some individuals (known as “persistent ...
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Hyper Immunoglobulin M Immunodeficiency (Dysgammaglobulinemia): PRESENCE OF IMMUNOGLOBULIN M SECRETING PLASMACYTOID CELLS IN PERIPHERAL BLOOD AND FAILURE OF IMMUNOGLOBULIN M IMMUNOGLOBULIN G SWITCH IN B CELL DIFFERENTIATION
... Such a subpopulation of lymphoid cells was not detected in normal peripheral blood and was unique for all patients with hyper IgM immunodeficiency studied.. T cells from all patients wer[r] ...
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