in situ hybridisation
Chromogenic in situ hybridisation for the assessment of HER2 status in breast cancer: an international validation ring study
... the treatment of women with HER2-positive breast cancer [15,16]. As responsiveness to trastuzumab therapy is directly linked to a patient's HER2 status, accurate determination of HER2 status is essential for identifying ...
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The use of fluorescence in situ hybridisation techniques in the diagnosis and prognosis of malignancy
... in situ hybridisation therefore correlated w ell w ith those from other techniques, show ing that there w as no N-myc am plification, b u t th at the tum our contained a pseudodiploid clone w ith a probable ...
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Red and white muscle development in the trout (Oncorhynchus mykiss) as shown by in situ hybridisation of fast and slow myosin heavy chain transcripts
... In this study, we have examined the development of slow and fast muscle in the trout myotome using in situ hybridisation of mRNAs specific to the slow and fast muscle lineages. Our results show that the ...
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Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses
... in situ hybridisation (FISH) has already been successfully implemented in clinical microbiology for the identification of various pathogens, including fungi ...The hybridisation of fixed fungi with ...
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In situ hybridisation for the detection of viral nucleic acids
... in situ hybridisation for achieving high resolution since the track length of the p-partlcle in nuclear emulsion is only ...localise hybridisation to particular areas using X-ray film, then coating ...
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A fluorescence in-situ hybridisation and molecular investigation of leukaemia associated abnormalities of chromosome 6q.
... GluR-6 through this mechanism occurs in ALL, sequence analysis of the GluR-6 exons and flanking intronic sequences was performed in patients and cell lines known to bear deletions [r] ...
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Introgression of yellow flower colour in Buddleja davidii by means of polyploidisation and interspecific hybridisation
... (Dirr 1998). Recent genomic in situ hybridisation (GISH) analysis proved the hybrid origin of B. weyeri- ana cv. Sungold (Van Laere et al. 2009). Other inter- specific hybrids in Buddleja were reported, ...
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The IL-12 family and dendritic cells : key regulators of immunity and immunological tolerance
... in-situ hybridisation experiments would be necessary to address this specific ...in-situ hybridisation would be useful in fully confirming the expression of this regulatory ...
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DNA Copy Number Variation in a Cohort of Healthy Australian Women
... genomic hybridisation (CGH), first developed in 1993 (58) provided the foundation for future technological ...in- situ hybridisation (FISH) which are applied to metaphase spreads of a normal ...
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Importance of the Secondary Genepool in Barley Genetics and Breeding
... In situ hybridisation, which combines cytogenetic and molecular methods, can be used to identify and locate introgressions transferred from Hordeum bulbosum ...in situ hybridisation (GISH) to ...
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Application of flow cytometry for enumerating individual bacterial cultures from a mixed culture system : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Philosophy in Food Technology at Massey University, Palmerston North, New Zealand
... in-situ hybridisation (FLOW-FISH) was investigated as a potential solution and successful enumeration was achieved within 1 day for a yoghurt microorganism, Streptococcus thermophilus (ST55), grown in M17 ...
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Identification of compounds with anti-convulsant properties in a zebrafish model of epileptic seizures
... increasingly apparent. Several recent studies have demonstrated that the zebrafish is particularly well suited to the analysis of epilepsy mechanisms and anti-epileptic drug discovery (Baraban et al., 2005; Berghmans et ...
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Genomic characterisation of a Fgf regulated gradient based neocortical protomap
... in situ hybridisation to whole-mount embryos and parasagittal embryo sections was carried out as described (Brent et ...in situ hybridisation was carried out as described using the same probes ...
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Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula
... Snap-frozen newly hatched and 11-day-old larvae were thawed at 4 ˚ C overnight in 4% paraformaldehyde phosphate-buffered saline. The head was dissected from the body and the eyes were removed. Whole-mount in-situ ...
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Cryopreservation and genetic damage : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Genetics at Massey University
... That Sister Chromatid Exchange, Micronucleus Assay and Fluorescence in situ Hybridisation can be performed on whole blood of radiation exposed veterans and non-exposed controls after a p[r] ...
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... Laboratory assessment of the status of Her-2/neu protein and oncogene in breast cancer specimens: comparison of immunohistochemistry assay with fluorescence in situ hybridisation assays.[r] ...
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Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of ? catenin in ovarian tumours
... in situ hybridisation probes to evaluate the presence of FRAT1 and used an anti-b-catenin antibody to evaluate by immunohistochemistry the expression levels and subcellular localisation of b-catenin in ...
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Fluorescence in situ hybridization as adjunct to cytology improves the diagnosis and directs estimation of prognosis of malignant pleural effusions
... Molecular analysis of the pleural fluid of patients using fluorescence in situ hybridisation to discriminate those with benign and malignant pleural effusions has not been well studied. We hypothesized that ...
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GABAA/benzodiazepine receptor distribution and subunit mRNA expression in human temporal lobe epilepsy
... 16 microcentrifuge tubes. A series of aqueous dilutions (20|il) o f [a-^^S]dATP were prepared ranging from approximately 2,000Bq/pl to lOBq/pl. lOpl of each stock dilution were added to a corresponding labelled ...
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Physical and genetic mapping in the proximal short arm of the X chromosome
... in situ hybridisation was 0.2ng/|xl to 2ng/pl of hybridisation mixture (Dr John Gosden, personal communication) ...of hybridisation mixture were used per ...Similar hybridisation ...
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