in-vitro transcription
In-Vitro Transcription and Transfection of HCV Genomic Replicons
... in-vitro transcription using MEGAscript Kit (Ambion, USA) ...reverse transcription (Thermo Fisher Scientific, ...In-vitro transcription and was transfected to grown Huh ...in ...
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Characterization of in vitro transcription and transcriptional products of measles virus.
... To determine whether the measles virus RNA transcripts synthesized in the in vitro transcription system represented RNAs coding for viral structural proteins, slot blot analysis was perf[r] ...
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An in-vitro transcription assay for development of Rotavirus VP7
... ter transcription efficiency was found when one extra nucleotide (A, T, C, and G) was placed before HDV ribozyme sequence (21, ...ficient transcription will be ...in vitro transcription, ...
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In vitro transcription of adenovirus.
... Early region II is unique among early regions of adenovirus for several reasons: i it is transcribed at the early and late stages of infection by initiation at different promoter sites 7[r] ...
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In vitro transcription of human pararotavirus.
... However, when pararotavirus was subjected to treatment with EDTA, even at lower concentrations and shorter times than those used for rotavirus, the inner protein shell that covers the vi[r] ...
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In vitro transcription catalyzed by heat-treated human rotavirus.
... To test whether a similar situation exists in human rotaviruses, we examined the effect of ribonucleoside triphosphate analogs on viral transcription.. Replacement of ATP by its analog /[r] ...
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In vitro transcription of two human rotaviruses.
... To assess whether all of the RNA segments were synthesized in vitro and to determine segment homologies between the two viral strains, preparations of labeled ssRNAs were hybridized to d[r] ...
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Multiple transcriptional regulatory domains in the human immunodeficiency virus type 1 long terminal repeat are involved in basal and E1A/E1B-induced promoter activity.
... These same promoter elements were required for both basal and ElA/ElB-induced levels of transcription in in vitro transcription reactions performed with cellular extracts prepared from c[r] ...
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Transcriptional regulation of three double-stranded RNA segments of bacteriophage phi 6 in vitro.
... These two apparently different patterns produced in in vitro transcription presumably reflect the two distinct in vivo transcription patterns; i.e., i similar amounts of three single-str[r] ...
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New procedure for the direct analysis of in vitro reverse transcription of Rous sarcoma virus RNA.
... The precise mechanism of this in vitro tranmor 18 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Based on the observation that in vitro transcription of Rous sarcoma v[r] ...
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Differential transcription of baculovirus late and very late promoters: fractionation of nuclear extracts by phosphocellulose chromatography.
... in vitro transcription system based on a cytidine-free cassette was developed for the late 39k gene and the very late polyhedrin gene of Autographa californica nuclear polyhedrosis virus ...of ...
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Polyadenylation of Influenza Virus mRNA Transcribed In Vitro from Model Virion RNA Templates: Requirement for 5′ Conserved Sequences
... in vitro assay where added templates lacking 59 vRNA sequences are competent transcription tem- plates, a trans-acting polymerase complex which initiates tran- scription without ever being bound to the 59 ...
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Sp1 transcription factor is required for in vitro basal and Tat-activated transcription from the human immunodeficiency virus type 1 long terminal repeat.
... specific transcription during the ...in vitro transcription ...basal transcription and Tat-medi- ated trans activation of the HIV-1 promoter in the salt fraction to the same extent as the ...
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Optimal Replication Activity of Vesicular Stomatitis Virus RNA Polymerase Requires Phosphorylation of a Residue(s) at Carboxy-Terminal Domain II of Its Accessory Subunit, Phosphoprotein P
... vivo transcription activity of the protein required phosphorylation of the residues in this domain (45), although low levels of in vitro transcription ac- tivity were obtained with domain I ...
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Expression and purification of vesicular stomatitis virus N-P complex from Escherichia coli: role in genome RNA transcription and replication in vitro.
... RNP transcription in ...RNA transcription, we purified the N-P complex from the pellet fraction and used it in an in vitro transcription reaction con- taining RNP purified from the ...in ...
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Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH
... in vitro transcription analysis established that SL RNA gene transcription essentially de- pends on a factor of six subunits that specifically binds to the SL RNA gene USE and comprises three SNAPc ...
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Ex Vivo and In Vitro Identification of a Consensus Promoter for VSG Genes Expressed by Metacyclic-Stage Trypanosomes in the Tsetse Fly
... of transcription, but the relevance of this to the meta- cyclic stage is ...in vitro transcription experi- ments, we saw a good signal from the ...in vitro transcription system, using ...
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Translational requirement of La Crosse virus S-mRNA synthesis: in vitro studies.
... To examine the possible effect of ongoing protein synthesis on LAC transcription in vitro, virion polymerase reactions were first carried out under conditions optimized for in vitro prot[r] ...
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An in vitro method for detecting genetic toxicity based on inhibition of RNA synthesis by DNA lesions
... Therefore, we tested the utility of qPCR for detecting RNA transcription inhibition by UV-induced DNA le- sions (Fig. 4a). qPCR is both powerful and sensitive and is used for a broad range of applications. ...
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The 5′ Untranslated Region of Rhopalosiphum padi Virus Contains an Internal Ribosome Entry Site Which Functions Efficiently in Mammalian, Plant, and Insect Translation Systems
... other aphids, but the virus genome does have some similarity to that of the comoviruses, which actively replicate in plants (11). Thus, it was possible that the reported lack of RhPV replication in plant cells might be ...
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