Indirect ELISA
Diagnosis of tuberculosis in an Indian population by an indirect ELISA protocol based on detection of Antigen 85 complex: a prospective cohort study
... for ELISA test using ROC ...the indirect ELISA method yielded 82% sensitivity (95% con- fidence interval [CI] 67 to 93%) and 86% specificity (95% CI, 57 to 98%) for the diagnosis of TB using the mAb ...
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Identification of Anaplasma ovis appendage associated protein (AAAP) for development of an indirect ELISA and its application
... A recombinant A. marginale major surface protein 5 (Msp5) based competitive inhibition enzyme-linked im- munosorbent assay (CI-ELISA) has been developed and shown to detect A. marginale-infected cattle, including ...
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Distinction of subtype specific antibodies against European porcine influenza viruses by indirect ELISA based on recombinant hemagglutinin protein fragment 1
... the indirect ELISAs but could as well have been caused by lack of specificity of the HI assays; problems with reproducibility and standardization of HI assays are notorious, especially when testing low-titred sera ...
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The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum
... direct ELISA for the detection of CAV ...an ELISA plates to evaluate its reactivity against chicken ...resulting indirect ELISA was then compared with a commercial ...the indirect ...
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Original Article In vitro expression and development of indirect ELISA for Capsid protein of duck circovirus without nuclear localization signal
... The positive serum of avian influenza virus (AIV), duck hepatitis virus (DHV), duck swollen head septicemia virus (DSHSV), Riemerella anatipestifer (RA), Escherichia coli (E. coli) and Salmonella enteritidis (S.E) ...
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Development of a New Indirect ELISA Method for Detection of Anti-Tuberculosis Antibodies in Human Serum
... strategies depending on the assay; In the case of anti-TB antibodies, by the application of multi-antigenic antigens (or multi-antigens) in order to enhance the sensitivity of the test, and using more specific types of ...
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Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect ELISA for diagnosis
... Methods: Here, we used recombinant Sarcoptes scabiei cofilin protein as an antigen to establish indirect ELISA. S. scabiei cofilin is highly homologous to Dermatophagoides farinae Der f 31 allergen (90 % ...
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Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast expressed antigens
... land, India, Bangladesh and other countries ([10,11]. AIV has become an important potential risk factor for human health. Since May 2005, the numbers of both affected countries and confirmed cases of influenza A (H5N1) ...
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GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA
... Early diagnostic methods based on excellent antigens are important for the control of coenurosis. The excellent serodiagnostic value of the GP50 antigen has been con- firmed in T. solium cysticercosis [12 – 16, 22]. ...
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A novel modified indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long term infections with diverse Babesia bovis strains
... conventional indirect ELISA ...conventional indirect ELISA. In addition, the MI-ELISA uses a HRP-conjugated rGST-SBP4 fusion protein as the detecting reagent, thus the poly-epitope ...
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An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus)
... including indirect immunofluorescence (IFAT, Mohn & Ødegaard 1977) and carbon im- munoassay (CIA, Mohn ...an ELISA on blue fox sera for ...new indirect ELISA with antigen produced from a ...
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Development of an Indirect ELISA Using Different Fragments of Recombinant Ncgra7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo.
... Methods: NcGRA7, from N. caninum tachyzoites was amplified. PCR product and pMAL-c2X plasmid were digested with EcoR1 restriction enzyme and ex- pressed in Escherichia coli to evaluate its competence for detection of ...
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Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV specific human IgG and IgM antibodies by indirect ELISA
... The total antibody sandwich-ELISA system which was developed by Jiao et al. was widely used in China because it is simple to perform, could be used to human and all kinds of animals. But the disadvantages of this ...
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Seroprevalence of human fascioliasis using indirect ELISA in isfahan district, central iran in 2013.
... Methods: Overall, 471 sera samples were collected from people recalled randomly to 20 health centers in the city of Isfahan and 10 related villages in 2014. Sera were examined using ELISA test. A questionnaire was ...
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Compared the effect of indirect ELISA and serum plate agglutination (SPA) test for the detection of Mycoplasma gallisepticum in chicken
... of ELISA and SPA test for MG antibody detection is only a screening tool for flocks but not for individual birds recommended by the World Organization for Animal Health ...
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Comparison of the sensitivity and cross-reactivity between heat-killed and formalin-killed cells during the development of indirect competitive ELISA for the detection of Salmonella Typhimurium
... Overall, the result indicate that antibody raised to FK cells showed approximately twice the sensitivity and approximately ten times the linear range compared to antibody raised to HK cells. This is also observed by an ...
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ELISA: a solid-phase immune assay
... - direct detection (primary antibody is conjugated with the enzyme/tag) Indirect ELISA – signal amplification. - indirect detection (labelling the secondary antibody - produced[r] ...
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AccuDiag HCV Ab ELISA
... 4. If, after retesting of the initially reactive samples, the assay results are negative, these samples should be considered as non-repeatable (false positive) and interpreted as negative. As with many very sensitive ...
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An overview on ELISA techniques for FMD
... Moreover, ELISA can simultaneously quantify 146S in many sam- ples and monitor whole virus particle in each step of vaccine manufacture as well as being used to test the effects of medium modification and ...
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Instruction Manual ELISA kit
... Cytokines, chemokines and granzymes are a group of signaling proteins that affect the behavior of cells when released. They are critically involved in various physiological processes such as immune regulation, cell ...
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