indirect enzyme linked immunosorbent assay
Indirect Enzyme Linked Immunosorbent Assay for Detection of Brucella melitensis Specific Antibodies in Goat Milk
... Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, ...
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Indirect enzyme linked immunosorbent assay for immunoglobulin G and four immunoassays for immunoglobulin M to Toxoplasma gondii in a series of heart transplant recipients
... Toxoplasma gondii infections in heart transplant recipients were monitored by indirect enzyme-linked immunosorbent assay for immunoglobulin G ELISA-IgG, indirect ELISA-IgM in serum IgM f[r] ...
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Interlaboratory evaluation of indirect enzyme linked immunosorbent assay, antibody capture enzyme linked immunosorbent assay, and immunoblotting for detection of immunoglobulin M antibodies to Toxoplasma gondii
... One hundred and fifteen serum samples from healthy laboratory personnel and 50 consecutive samples from 19 patients with anamnestic clinical signs of toxoplasmosis were assayed by four l[r] ...
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Sensitivity and specificity of viral immunoglobulin M determination by indirect enzyme linked immunosorbent assay
... Several sera containing no rubella-specific IgM, rubella-specific IgG, or IgM-RF and giving rise to false positivity in the rubella IgM ELISA were pretreated with various amounts of anti[r] ...
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Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis
... and His tag (the total molecular mass of the tags was 18 kDa) at the carboxyl terminus. The restriction enzyme digestion and DNA sequencing analysis showed the bp26 gene was inserted into pET32a(⫹) vector ...
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Evaluation of specificity of indirect enzyme linked immunosorbent assay for diagnosis of human Q fever
... Ninety-five acute- and convalescent-phase serum samples Si and S2, respectively from these 48 patients with a fourfold or greater rise or fall in antibody titer by the IFA test and with [r] ...
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Development of a New Indirect ELISA Method for Detection of Anti-Tuberculosis Antibodies in Human Serum
... substantial in developing countries where TB has very high incidence rate. Methods: An Indirect Enzyme-linked immunosorbent Assay (ELISA) was established to detect serum antibodies ...
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Development of an Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of Toxoplasma gondii Infection
... Dense granule antigen proteins derived from Toxoplasma gondii (TgGRAs) are potential antigens for the development of diag- nostic tools. TgGRA7 and TgGRA14 were detected in the peritoneal fluid of T. gondii-infected ...
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Article: Seroprevalence of canine brucellosis in Dhaka city corporation area, Bangladesh
... Abstract: An investigation was conducted to determine the seroprevalence of brucellosis in pet dogs of Dhaka City Corporation area. A total of 50 sera samples from pet dogs were collected. Additional data on risk factors ...
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Occurrence and Potential Diagnostic Applications of Serological Cross-Reactivities between Brucella and Other Alpha-Proteobacteria
... an indirect enzyme-linked immunosorbent assay (ELISA) the reactivities of brucellosis sera against cytosolic (CYT) and membrane (MA) antigens from these nonpathogenic bacteria, and we ...
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Antibody Responses to Recombinant Protein Fragments of the Major Outer Membrane Protein and Polymorphic Outer Membrane Protein POMP90 in Chlamydophila abortus-Infected Pregnant Sheep
... (rOMP90-4 indirect enzyme-linked immunosorbent assay [iELISA]) and one based on the variable segment 2 (VS2) region of the major outer membrane protein (MOMP) (MOMP VS2 iELISA) were ...
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Article: Detection of avian reovirus antibodies in layer birds of small scale commercial farms in Dinajpur district of Bangladesh
... The indirect enzyme-linked immunosorbent assay (iELISA) was performed according to the manufacturer’s instruction using ARV pre coated plates and pre-diluted, ready to use reagents and ...
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Modification of a direct enzyme linked immunosorbent assay for the detection of immunoglobulin G and M antibodies to pneumococcal capsular polysaccharide
... In contrast to the usual indirect enzyme-linked immunosorbent assay ELISA method for detection of antibody responses, a modified direct ELISA technique was used to measure Immunoglobulin[r] ...
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Monoclonal antibody mediated, immunodiagnostic competitive enzyme linked immunosorbent assay for equine monocytic ehrlichiosis
... A comparison of ODs obtained by CELISA and indirect enzyme-linked immunosorbent assay ELISA indicated a marked tendency of positive and negative samples to cluster separately with respec[r] ...
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Evaluation of Vircell Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Detection of Antibodies against Legionella pneumophila
... Usually both IgM and IgG can be detected in samples of patients during the course of their illness, but in a significant proportion of patients, no IgG is detectable (6). Studies have shown that many patients produce ...
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Monoclonal antibody recognizing a species specific protein from Streptococcus pneumoniae
... However, MAbs to the 37-kDa antigen bound whole cells in the enzyme-linked immunosorbent assay and the indirect immunofluorescence assay.. Antibody-binding epitopes of the antigen are pr[r] ...
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Enzyme linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double sandwich enzyme linked immunosorbent assay
... Of 80 single sera with high antibody titer to toxoplasma in indirect immunofluorescence and complement fixation, 40 were positive in the direct ELISA for ToxIgM, 36 were positive in the [r] ...
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Immunity to human cytomegalovirus measured and compared by complement fixation, indirect fluorescent antibody, indirect hemagglutination, and enzyme linked immunosorbent assays
... A comparison of cytomegalovirus antibody titers was made between the complement fixation test, a commercially available enzyme-linked immunosorbent assay, an indirect immunofluorescent t[r] ...
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Comparison of indirect immunofluorescent antibody assay, enzyme linked immunosorbent assay, and Western immunoblot for the diagnosis of Lyme disease in dogs
... Enzyme-linked immunosorbent assay ELISA, indirect immunofluorescent-antibody assay IFA, and Western immunoblot were used to test serum samples from 128 dogs for the presence of antibody [r] ...
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Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus
... The rE2 of CHIKV was used as an antigen in an indirect ELISA for diagnosis of CHIKV infection. Concentrations of rE2, from 0.5 μg/ml to 8 μg/ml, diluted in 0.05 M Carbonate-Bicarbonate buffer pH 9.6 ...
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