inositol phosphate(s)
Constitutive Inositol Phosphate Formation in Cytomegalovirus-Infected Human Fibroblasts Is due to Expression of the Chemokine Receptor Homologue pUS28
... ated inositol phosphate formation at early times in HCMV infection (12 and 24 h ...constitutive inositol phos- phate formation seen upon transfection with a maximally ef- fective amount of the cDNA ...
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Matrix Domain Modulates HIV-1 Gag's Nucleic Acid Chaperone Activity via Inositol Phosphate Binding
... annealing to the PBS, we characterized the capabilities of WT and variant Gag proteins to bind, aggregate, and desta- bilize NA helices. Basic regions at both ends of Gag (i.e., MA and NC) have previously been shown to ...
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Evidence for attenuation of myo inositol uptake, phosphoinositide turnover and inositol phosphate production in aortic vasculature of rats during pregnancy
... 45 s of incubation with 10(-7) M arginine vasopressin, however, the fold-stimulation of [3H]inositol trisplus tetrakisphosphate was reduced in aortae from gravid rats, when compared with vessels from virgin ...
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Inositol 1,4,5 trisphosphate and inositol 1,3,4,5 tetrakisphosphate are second messenger targets for cardioactive neuropeptides encoded on the FMRFamide gene
... the inositol responses to FMRFamide would be similar to that resulting from FMRFamide application to the whole ...30 s application of FMRFamide (N=22 ...total inositol phosphate production ...
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Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture
... -inositol phosphate (phytate) is one of the major forms of organic soil phosphorus making up to 50% of all organic P in soil [8] [9] [10] ...-inositol phosphate and other compounds are ...
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Salmeterol and cytokines modulate inositol-phosphate signalling in Human airway smooth muscle cells via regulation at the receptor locus
... 2–3 days until ~80% confluent. Complete medium was replaced with serum free medium and the cells were grown for a further 24 hours followed by the addition of cytokines or drugs (as described above). Isolation of cells ...
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Phosphatidylinositol metabolism in cells transformed by polyomavirus middle T antigen.
... We did not find consistent changes in total inositol phosphate levels or in the levels of Ins1,4P2 or Ins1,4,5P3 which, when normalized to the level of PtdIns present in cells, correlate[r] ...
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Endothelin stimulates phospholipase C, Na+/H+ exchange, c fos expression, and mitogenesis in rat mesangial cells
... A recently described peptide hormone, endothelin, is a potent vasoconstrictor, but it is unclear whether endothelin has other biological actions. These experiments extend the range of biological actions of endothelin to ...
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Expression of 1L-myo-Inositol -1-Phosphate Synthase (EC 5.5.1.4) is Deregulated in the Cerebellum of Curly Tail Mutant Mice
... of inositol, overall, in the ct-CT cerebellum when compared to levels of inositol in the cerebellum of CBA and st-CT mice ...of inositol in the CNS and findings that suggest inositol ...
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Endothelin receptor is coupled to phospholipase C via a pertussis toxin insensitive guanine nucleotide binding regulatory protein in vascular smooth muscle cells
... Treatment of A-10 cells with pertussis toxin induces ADP-ribosylation of a 41,000-D membrane protein but fails to block the ET-induced increases in inositol phosphate production and Ca2+[r] ...
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Transfection mediated expression of a dominant cAMP resistant phenotype in the opossum kidney (OK) cell line prevents parathyroid hormone induced inhibition of Na phosphate cotransport A protein kinase A mediated event
... overexpression of the mutant regulatory unit. Protein kinase A from these cells had a 20-fold increase in the concentration of cAMP required for half-maximal activation, 2.8 microM vs. 0.15 microM for wild type cells. In ...
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Transcellular activation of platelets and endothelial cells by bioactive lipids in platelet microparticles
... concentration, inositol phosphates, and thromboxane formation evoked by the microparticles and by inhibition of microparticle-induced platelet aggregation by an inhibitor of protein kinase ...
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D-Chiro-Inositol Glycans in Insulin Signaling and Insulin Resistance
... activation of a GPI-phospholipase, possi- bly GPI phospholipase D (29). These re- sponses to insulin are less well recog- nized. The action of the phospholipase releases INS-2 from a GPI lipid precursor that may be in ...
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A cell factory of Bacillus subtilis engineered for the simple bioconversion of myo-inositol to scyllo-inositol, a potential therapeutic agent for Alzheimer's disease
... of inositol stereoisomers in the medium After bioconversion, aliquots of the culture medium were subjected to gas chromatography-time-of-flight mass spectrometry (GC-TOFMS) analysis as described [20] but with some ...
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RNAi mediated down regulation of myo-inositol-3-phosphate synthase to generate low phytate rice
... Low phytic acid phenotypes are often associated with downstream impacts on seed morphology and germin- ation. From previous studies it is evident that reduction of phytate mediated by down regulation of MIPS might result ...
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A Noninvasive Comparison Study between Human Gliomas with IDH1 and IDH2 Mutations by MR Spectroscopy
... The excess production of 2-HG or reduced -KG in IDH mutations (Figure 1A) suggests that 2- HG can be directly involved in promoting tumorigenesis [3]. However, recent studies found that it would be insufficient to ...
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CLONING AND CHARACTERIZATION OF MYO - INOSITOL-1-PHOSPHATE SYNTHASE (MIPS1) GENE IN DEVELOPING SEEDS OF GLYCINE MAX
... myo- inositol, which functions as a major storage form of phosphorous in plant ...(myo- inositol-1- phosphate synthase) catalyzes the first and rate limiting step in phytic acid ...
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Knockdown of interleukin-10 induces the redistribution of sigma1-receptor and increases the glutamate-dependent NADPH-oxidase activity in mouse brain neurons
... monoclonal inositol 1,4,5-trisphosphate receptor (IP3R) antibody, (Abcam, UK), or with goat polyclonal NR2B glutamate receptor ( ε 2) antibody (Santa Cruz, ...
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Mechanistic studies on myo inositol monophosphatase
... uracil phosphate Intermediate (132) may have been sufficiently unstable In aqueous solution to undergo Immediate non-enzymic hydrolysis to uridine and Inorganic ...
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CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2 N141I neurons
... DNA: Deoxyribonucleic acid; DPBS: Dulbecco ’ s phosphate-buffered saline; DPBST: Dulbecco ’ s phosphate-buffered saline + 0.1% Triton X-100; EGTA: Ethy- lene-bis(oxyethylenenitrilo)tetra[r] ...
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