insect cell-baculovirus system
Production and purification of VP2 protein of porcine parvovirus expressed in an insect baculovirus cell system
... a baculovirus system, although baculovirus expression systems are likely to be more costly than bacterial expression systems for producing viral proteins and may present difficulty in purifying ...
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A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
... Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant ...a baculovirus expression vector encoding a ...
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... the baculovirus insect cell system has attracted wide attention as vectors for high level and faithful expression of a variety of heterologous ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... host cell invasion (21, 22). The major immunogenic B-cell and T-cell epitopes on RAP-1 are conserved among all strains tested, but they are not conserved between different species (5, ...in ...
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Baculovirus coinfection strategy for improved galactosylation of recombinant glycoprotein produced by insect cell culture
... Clearly, the in vivo galactosylation as performed did not show encouraging result due to the limitation of the sugar nucleotide. Thus, another strategy, post harvest in vitro galactosylation was developed to improve the ...
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Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... The baculovirus expression system has been used extensively to express large quantities of proteins that are antigenically similar to their native counterparts and can be used in stan- dardized assays to ...
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Lymantria dispar Nucleopolyhedrovirushrf-1 Expands the Larval Host Range of Autographa californica Nucleopolyhedrovirus
... in insect hemolymph indicated that Autographa californica NPV (AcNPV) nucleocapsids budded through the midgut basal lamina into the hemocoel of Tricho- plusia ni larvae within ...tracheal system, which ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... the Baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) downstream of the strong polyhedrin ...the insect cell line, Spodoptera frugiperda (Sf9) ...
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Identification of baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line.
... and cell- mediated ...defense system against viral infection, the shutoff of protein ...other baculovirus genes that have been implicated in host range determination, p35 (10, 11), the p143 helicase ...
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Optimization of canine interleukin 12 production using a baculovirus insect cell expression system
... a baculovirus vector devoid of cathepsin and chitinase genes (in contrast to the classi- cal baculovirus vector), contributed to increased produc- tion of secreted recombinant ...four baculovirus ...
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Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of NeosporaInfection
... a baculovirus suggests that the signal peptide is cleaved at the same cleavage site in both insect cells and ...in insect cells ...the baculovirus-Sf9 cell expression ...
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Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... mid, designated pGEX/BC134, was used to produce a recom- binant BC134 protein fused with glutathione S-transferase (GST) (designated the GST-BC134 protein) in the E. coli BL21 strain (Stratagene) according to standard ...
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Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
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Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... In this study, a recombinant baculovirus carrying the gene encoding SAG2 of T. gondii was constructed, and the recom- binant SAG2 was expressed in Sf9 cells. The molecular mass of the S-rSAG2 protein is 19 kDa, ...
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Identification and Expression of a 50 Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... advantages of high degrees of sensitivity and specificity, but the disadvantage of the test is the requirement for specialized laboratory equipment and facilities and well-trained laboratory personnel. On the other hand, ...
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Recombinant virus vaccine for bluetongue disease in sheep.
... Bluetongue virus tubules made in insect cells by recombinant baculovirus: expression of NS1 gene of bluetongue virus serotype 10.. Structure of the bluetongue virus capsid.[r] ...
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Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ...
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Recombinant type 5 adenoviruses expressing bovine parainfluenza virus type 3 glycoproteins protect Sigmodon hispidus cotton rats from bovine parainfluenza virus type 3 infection.
... At 5 days after viral inoculation, lungs had a mottled ap- pearance upon gross examination of the tissue. Immunohisto- chemically, viral antigen was demonstrated to be widespread within the airway epithelium, and ...
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A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins
... like baculovirus F proteins, Dm-cF and the proteins encoded by the f genes from ...the baculovirus F proteins from group I NPVs and GVs (those considered “fusion competent”) (22, 39, 42, 48, 49), the F ...
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Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system.
... We report the successful production of the major capsid antigen VP6 of the simian rotavirus SAl1 in insect cells, using the baculovirus Autographa californica nuclear polyhedrosis virus [r] ...
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