insect cells baculovirus expression system
The effect of time and optimization of the baculovirus expression system for more efficient recombinant protein production of porcine epidemic diarrhea virus in insect cells
... the cells (O'Reilly et al., 1994). However, only about 10% of the cells are transfected with the bacmid or are infected by the virus during the rst ...the insect cells are not infected and ...
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Optimization of canine interleukin 12 production using a baculovirus insect cell expression system
... Bac-to-Bac system, Invitrogen, ...AcBacΔCC-sccaIL-12opt baculovirus constructs were obtained by transfecting Sf-9 cells with the corresponding bacmids using a Lipofectamine rea- gent, following the ...
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A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins
... for expression in Escherichia coli and D. melanogaster S2 cells using the Gateway system ...an expression plasmid for ex- pressing Dm-cF-GFP under the control of a metalothionine promoter in ...
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PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells
... overexpression system, in effect preventing proper ...in insect cells, we created three recombinant baculoviruses ...third baculovirus, Myr0, expresses RSV Gag with a wild-type ...
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Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... Bac-to-Bac expression system was chosen because it is a valid and multi- purpose system for the production of recombinant ...an expression cassette into a baculovirus shuttle vector ...
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Expression and characterization of the human erythrocyte glucose transporter in insect cells using a recombinant baculovirus
... the insect cells was found to be most potently inhibited by phloretin, the aglucone of phlorizin, which inhibited w ith an apparent K, of ...the insect cells (apparent K; = ...the ...
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Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ...
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Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... The B. bovis rhoptry-associated protein 1 (RAP-1) gene encoding a 60-kDa merozoite apical membrane polypeptide was identified by Suraez et al. (23). The function of RAP-1 is poorly understood, but it is believed that ...
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... The baculovirus expression vector system (BEVS) is frequently a method of choice for the expression of recombinant mammalian proteins (O’Reilly et ...the insect host cells ...
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Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... The baculovirus expression system has been used extensively to express large quantities of proteins that are antigenically similar to their native counterparts and can be used in stan- dardized ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... (BSC-1 cells) were grown as a monolayer in DMEM (Himedia, India) supplemented with 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, and 100 U penicillin and 100 µg streptomycin per ml at ...infected cells ...
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Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
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Histidine at Residue 99 and the Transmembrane Region of the Precursor Membrane prM Protein Are Important for the prM-E Heterodimeric Complex Formation of Japanese Encephalitis Virus
... by baculovirus coexpression of prM and E in trans in Sf9 insect cells as analyzed by anti-E antibody immunoprecipitation and sucrose gradient sedimentation ...reduced expression or different ...
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Responses of insect cells to baculovirus infection: protein synthesis shutdown and apoptosis.
... infected cells that were undergoing extensive apoptosis at 24 to 28 h ...Ld652Y cells were infected at an MOI of 10 with v D p35/hrf-1 or vAc/hrf-1 (18) in the presence or absence of aphidicolin and ...
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Identification and characterisation of the interaction between Wiskott-Aldrich syndrome protein (WASP) and c-Src kinases
... I initially was unable to co-immunoprecipitate endogenous WASP from cell extracts, with any of its potential binding partners. A variety of cell lines, subjected to different stimuli were used, together with various ...
283
Identification and Expression of a 50 Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... Diagnosis of B. gibsoni infection in dogs by ELISA with recombinant P50 as antigen. To evaluate whether recombinant P50 expressed by baculovirus can be a suitable antigen for diagnosis of B. gibsoni infection in ...
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Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ...
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Recombinant type 5 adenoviruses expressing bovine parainfluenza virus type 3 glycoproteins protect Sigmodon hispidus cotton rats from bovine parainfluenza virus type 3 infection.
... Seven days postinoculation, both bronchiolitis and intersti- tial pneumonia were seen in the lungs of rats. Multiple foci of interstitial pneumonia were present and often were centered around terminal bronchioles ...
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Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats
... four baculovirus genera aligned with a set of well-defined insect PUR␣ proteins are illustrated in ...between baculovirus VP1054 and insect PUR␣ proteins are summarized in ...PUR␣, ...
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Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952.
... DISCUSSION The primary findings of this report are that i the full-length BPP from HCMV strain AD169 can be overexpressed as a recombinant baculovirus in Sf9 insect cells, ii baculovirus[r] ...
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